RAW STARCH-DEGRADING AMYLASE FROM BACTERIA IN KARANG MUMUS RIVER, SAMARINDA

Astuti, Winni; Adawiyah, Rabiatul; Putri, Amanda Aulia; Pratiwi, Djihan Ryn

doi:10.15575/ak.v11i2.39438

Jurnal

RAW STARCH-DEGRADING AMYLASE FROM BACTERIA IN KARANG MUMUS RIVER, SAMARINDA

al Kimiya: Jurnal Ilmu Kimia dan Terapan · Vol. 11 No. 2 (2024) · hlm. 141–146

DOI: 10.15575/ak.v11i2.39438
ISSN: 2407-1927, 2407-1897
Terbit: 2024-12-30
Penerbit: Department of Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung
Bahasa: eng

Abstrak

Raw starch-degrading amylase (RSDA) is an enzyme that has the ability to degrade starch granules into glucose without undergoing gelatinization. This research was conducted to explore and characterize extracellular RSDA-producing bacteria from body water of the Karang Mumus River, Samarinda. RSDA activity was analyzed qualitatively in nutrient agar containing 1% starch granules, bacterial colonies with RSDA activity are clear halos around bacterial colonies after the plates were flooded with iodine solution. Five of the 14 bacterial colonies secreted an RSDA extracellularly. The RSDA enzyme from the 5 bacteria was tested for amylase activity using the Dinitrosalicylic Acid (DNS) method. Bacteria with colony code KM 5 had the highest RSDA activity of 0.332 U/mL.The optimum working conditions for RSDA are at pH 5 and a temperature of 40Â°C. Identification of bacterial genotypes using the 16S rRNA gene showed that KM5 was Klebsiella sp, referred to as Klebsiella KM5.

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Universitas Islam Negeri Palangka Raya
Hasanuddin University
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Rekan penulis	Organisasi	Artikel bersama
Syamsuddin Toaha	Hasanuddin University	1
Kasbawati Kasbawati	Hasanuddin University	1
Muhammad Norhadi	Afiliasi tidak diketahui	1
Abdul Khair	Universitas Islam Negeri Palangka Raya	1
Zainatul Fatmah	Afiliasi tidak diketahui	1
Muhammad Norhadi	Universitas Islam Negeri Palangka Raya	1
Rabiatul Adawiyah	Universitas Islam Negeri Palangka Raya	1
Zainatul Fatmah	Universitas Islam Negeri Palangka Raya	1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.uinsgd.ac.id/index.php/ak/oai, pertama di-harvest 7 Nov 2026, terakhir terlihat 8 Nov 2026.

Metadata asli (oai_dc)

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	<dc:title xml:lang="en-US">RAW STARCH-DEGRADING AMYLASE FROM BACTERIA IN KARANG MUMUS RIVER, SAMARINDA</dc:title>
	<dc:creator>Astuti, Winni</dc:creator>
	<dc:creator>Adawiyah, Rabiatul</dc:creator>
	<dc:creator>Putri, Amanda Aulia</dc:creator>
	<dc:creator>Pratiwi, Djihan Ryn</dc:creator>
	<dc:subject xml:lang="en-US">Raw starch degrading amylase (RSDA)</dc:subject>
	<dc:subject xml:lang="en-US">Karang Mumus River</dc:subject>
	<dc:subject xml:lang="en-US">Dinitrosalicylic Acid</dc:subject>
	<dc:subject xml:lang="en-US">Klebsiella pneumoniae</dc:subject>
	<dc:description xml:lang="en-US">Raw starch-degrading amylase (RSDA) is an enzyme that has the ability to degrade starch granules into glucose without undergoing gelatinization. This research was conducted to explore and characterize extracellular RSDA-producing bacteria from body water of the Karang Mumus River, Samarinda.  RSDA activity was analyzed qualitatively in nutrient agar containing 1% starch granules, bacterial colonies with RSDA activity are clear halos around bacterial colonies after the plates were flooded with iodine solution. Five of the 14 bacterial colonies secreted an RSDA extracellularly. The RSDA enzyme from the 5 bacteria was tested for amylase activity using the Dinitrosalicylic Acid (DNS) method. Bacteria with colony code KM 5 had the highest RSDA activity of 0.332 U/mL.The optimum working conditions for RSDA are at pH 5 and a temperature of 40Â°C. Identification of bacterial genotypes using the 16S rRNA gene showed that KM5 was Klebsiella sp, referred to as Klebsiella KM5.</dc:description>
	<dc:publisher xml:lang="en-US">Department of Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung</dc:publisher>
	<dc:date>2024-12-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.uinsgd.ac.id/index.php/ak/article/view/39438</dc:identifier>
	<dc:identifier>10.15575/ak.v11i2.39438</dc:identifier>
	<dc:source xml:lang="en-US">al Kimiya: Jurnal Ilmu Kimia dan Terapan; Vol. 11 No. 2 (2024): al Kimiya: Jurnal Ilmu Kimia dan Terapan; 141-146</dc:source>
	<dc:source>2407-1927</dc:source>
	<dc:source>2407-1897</dc:source>
	<dc:source>10.15575/ak.v11i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.uinsgd.ac.id/index.php/ak/article/view/39438/12492</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2025 Winni Astuti, Rabiatul Adawiyah, Amanda Aulia Putri, Djihan Ryn Pratiwi</dc:rights>
</oai_dc:dc>