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Isolation and Detection of Bacteriocin-Like Inhibitory Substances-Producing Bacteria from Fermented Mare’s Milk Sumbawa

Jurnal Biodjati · Vol. 4 No. 1 (2019) · hlm. 21–30

DOI
10.15575/biodjati.v4i1.4194
ISSN
2541-4208, 2548-1606
Terbit
2019-05-28
Penerbit
UIN Sunan Gunung Djati Bandung
Bahasa
eng

Abstrak

  Bacteriocin-like inhibitory substances (BLIS) produced by bacteria is a promising future food preservative agent. This study aimed to obtain bacterial strains that can produce broad-spectrum antibacterial agents and identify the best BLIS producer species based on 16S rDNA sequences. The bacterial strains were isolated from fer-mented mare’s milk using MRS and M17 agar medium. The isolates then were initially screened based on its antibacterial activity of crude cells against Staphylococcus aureus ATCC 6538. The selected strains were cultured and harvested for its cell-free supernatant (CFS). The pH of CFS was adjusted to 6.5 then used for antibacterial activity as-says against ten pathogenic bacteria. Also, the proteinaceous nature of BLIS compound was confirmed by testing with proteinase K. The gDNA of selected isolates was extracted and the 16S rDNA was am-plified using the polymerase chain reaction method then sequenced. The 16S rDNA sequences of the selected strains were used to identify the species using BLAST nucleotides from NCBI then the phylogenetic trees were constructed. 32 isolates was obtained, but only three iso-lates (BC9, SB7, and DC12) were selected as a result of antibacterial screening for further assays. The neutralized-CFS (N-CFS) of these isolates exhibited broad-spectrum antibacterial activity. The N-CFS could be assumed as BLIS. The isolate of BC9 was identified as Ba-cillus amyloliquefaciens strain BC9 that has 99.99 % similarity with B. amyloliquefaciens KC-1, SB7 was Lactobacillus plantarum strain SB7 that has 99.99 % similarity with Lb. plantarum JMC 1149T, and DC12 was Lactobacillus rhamnosus strain DC12 that has 100 % sim-ilarity with Lb. rhamnosus DSM 20021T. Thus, the BLIS produced by those strains is potential for future food and beverages preservations.Â

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  • University of Brawijaya
  • Universitas Maarif Hasyim Latif
  • North Carolina Agricultural and Technical State University
  • Universitas Cokroaminoto Palopo
  • Universitas Katolik Widya Mandira
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Rekan penulisOrganisasiArtikel bersama
Muhaimin Rifa’iUniversity of Brawijaya3
Irfan MustafaUniversity of Brawijaya2
Nashi WidodoUniversity of Brawijaya2
Dwi Purno WidekdoUniversity of Brawijaya2
Agung Putra PurwantoUniversity of Brawijaya2
Nuris MaulidiyahUniversity of Brawijaya2
Elsa Mega SuryaniUniversitas Maarif Hasyim Latif1
Hikmah MaulidiyahUniversity of Brawijaya1
Salam Adnan IbrahimNorth Carolina Agricultural and Technical State University1
Suharjono SuharjonoUniversity of Brawijaya1
Elok ZubaidahUniversity of Brawijaya1
Eka Pratiwi TenriawaruUniversitas Cokroaminoto Palopo1
Endang ArisoesilaningsihUniversity of Brawijaya1
Eufrasia R. A. LengurUniversitas Katolik Widya Mandira1
Eko WidodoUniversity of Brawijaya1
Farida RachmawatiUniversity of Brawijaya1
Sri WidyartiUniversity of Brawijaya1
Aris SoewondoUniversity of Brawijaya1
Sri RahayuUniversity of Brawijaya1
Fikriya Novita SariUniversity of Brawijaya1
Sefihara Paramitha SaktiUniversity of Brawijaya1
Osfar SjofjanUniversity of Brawijaya1
Sulistya Ika RamadhaniAfiliasi tidak diketahui1
Aleyda WahyudiAfiliasi tidak diketahui1
Giral Baines MoraUniversity of Brawijaya1
Dewi PermatasariUniversity of Brawijaya1
Maharani Pertiwi KoentjoroUniversity of Brawijaya1
Syarafin MahiraUniversity of Brawijaya1
asep awaludin prihantoAfiliasi tidak diketahui1
Yoga Dwi JatmikoAfiliasi tidak diketahui1
Rika Gian MeliantiAfiliasi tidak diketahui1
Asep Awaludin PrihantoUniversity of Brawijaya1
Rika Gian MeliantiUniversity of Brawijaya1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.uinsgd.ac.id/index.php/biodjati/oai, pertama di-harvest 7 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Isolation and Detection of Bacteriocin-Like Inhibitory Substances-Producing Bacteria from Fermented Mare’s Milk Sumbawa</dc:title>
	<dc:creator>Mulyawati, Alifia Issabella</dc:creator>
	<dc:creator>Ardyati, Tri</dc:creator>
	<dc:creator>Jatmiko, Yoga Dwi</dc:creator>
	<dc:subject xml:lang="en-US">bacteriocin-like inhibitory substances</dc:subject>
	<dc:subject xml:lang="en-US">Bacillus amyloliquefaciens</dc:subject>
	<dc:subject xml:lang="en-US">Lactobacillus plantarum</dc:subject>
	<dc:subject xml:lang="en-US">Lactobacillus rhamnosus</dc:subject>
	<dc:subject xml:lang="en-US">preservative agents</dc:subject>
	<dc:subject xml:lang="en-US">fermented mare’s milk</dc:subject>
	<dc:description xml:lang="en-US">  Bacteriocin-like inhibitory substances (BLIS) produced by bacteria is a promising future food preservative agent. This study aimed to obtain bacterial strains that can produce broad-spectrum antibacterial agents and identify the best BLIS producer species based on 16S rDNA sequences. The bacterial strains were isolated from fer-mented mare’s milk using MRS and M17 agar medium. The isolates then were initially screened based on its antibacterial activity of crude cells against Staphylococcus aureus ATCC 6538. The selected strains were cultured and harvested for its cell-free supernatant (CFS). The pH of CFS was adjusted to 6.5 then used for antibacterial activity as-says against ten pathogenic bacteria. Also, the proteinaceous nature of BLIS compound was confirmed by testing with proteinase K. The gDNA of selected isolates was extracted and the 16S rDNA was am-plified using the polymerase chain reaction method then sequenced. The 16S rDNA sequences of the selected strains were used to identify the species using BLAST nucleotides from NCBI then the phylogenetic trees were constructed. 32 isolates was obtained, but only three iso-lates (BC9, SB7, and DC12) were selected as a result of antibacterial screening for further assays. The neutralized-CFS (N-CFS) of these isolates exhibited broad-spectrum antibacterial activity. The N-CFS could be assumed as BLIS. The isolate of BC9 was identified as Ba-cillus amyloliquefaciens strain BC9 that has 99.99 % similarity with B. amyloliquefaciens KC-1, SB7 was Lactobacillus plantarum strain SB7 that has 99.99 % similarity with Lb. plantarum JMC 1149T, and DC12 was Lactobacillus rhamnosus strain DC12 that has 100 % sim-ilarity with Lb. rhamnosus DSM 20021T. Thus, the BLIS produced by those strains is potential for future food and beverages preservations. </dc:description>
	<dc:publisher xml:lang="en-US">UIN Sunan Gunung Djati Bandung</dc:publisher>
	<dc:date>2019-05-28</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.uinsgd.ac.id/index.php/biodjati/article/view/4194</dc:identifier>
	<dc:identifier>10.15575/biodjati.v4i1.4194</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Biodjati; Vol. 4 No. 1 (2019): May; 21-30</dc:source>
	<dc:source xml:lang="id-ID">Jurnal Biodjati; Vol 4 No 1 (2019): May; 21-30</dc:source>
	<dc:source>2541-4208</dc:source>
	<dc:source>2548-1606</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.uinsgd.ac.id/index.php/biodjati/article/view/4194/2714</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2019 Jurnal Biodjati</dc:rights>
</oai_dc:dc>