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Genetic Profiling of Sida rhombifolia Originated from Several Indonesian Ethnicities Based on Sequence-Related Amplified Polymorphism Markers

Jurnal Biodjati · Vol. 6 No. 2 (2021) · hlm. 203–212

DOI
10.15575/biodjati.v6i2.14553
ISSN
2541-4208, 2548-1606
Terbit
2021-11-29
Penerbit
UIN Sunan Gunung Djati Bandung
Bahasa
eng

Abstrak

Sida rhombifolia is one of wild flowering plants that grows easily in many habitats with moderate humidity, with some usefulness in traditional medicine. Genetic characterization of Sida rhombifolia accessions originated from 12 ethnicities of Indonesia was analyzed based on Sequence-Related Amplified Polymorphism (SRAP) Markers. The genomic DNA were extracted from leaf samples and then were characterized by using the SRAP marker system according to Li and Quiros (2001). Nine pairs of SRAP primer resulted high polymorphic bands and were used in the genetic profiling. The data analysis was performed using GenAlEx to calculate genetic distance, Principal coordinate analysis, and Analysis of Molecular Variance (AMOVA), also using POPGENE to assess genetic diversity (Hs and Ht) and Nm to predict gene flow among populations. The coordinate analysis showed that the accessions originated from ethnicities along Wallacean line tend to differ genetically from most other locations. However, the results of analysis of molecular variance suggested that there were only slight differences (0.1%) found between ethnicities, while most genetic variances (99.9%) were found mostly among accessions within populations. The results suggested that there was an extensive genetic flow and plant spreading among Sida rhombifolia plant populations, resulting more homogenous genetic characters among most populations, while high diversity within population. The calculation of the number of migration (Nm = 1.7341) confirmed that the high rate of gene flow had occurred between populations.

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  • Kementerian Kesehatan Republik Indonesia
  • National Research and Innovation Agency
  • Sunan Kalijaga State Islamic University Yogyakarta
  • Universitas Halu Oleo
  • University of Bengkulu
  • Universitas Gadjah Mada
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  • Organisasi berbeda
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Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Rohmat MujahidKementerian Kesehatan Republik Indonesia2
Yuli WidiyastutiNational Research and Innovation Agency2
Abdul KadirUniversitas Halu Oleo1
Nurul IstiqomahAfiliasi tidak diketahui1
Nasrulloh NasrullohAfiliasi tidak diketahui1
Nina KurnianingrumKementerian Kesehatan Republik Indonesia1
Dian SusantiKementerian Kesehatan Republik Indonesia1
Aditya Dwi Permana PutraNational Research and Innovation Agency1
Devi SafrinaKementerian Kesehatan Republik Indonesia1
Nur Rahmawati WijayaKementerian Kesehatan Republik Indonesia1
M Bakti Samsu AdiNational Research and Innovation Agency1
Rizal Maarif RukmanaNational Research and Innovation Agency1
Sari HaryantiKementerian Kesehatan Republik Indonesia1
Usman SiswantoUniversity of Bengkulu1
Ratna SusandariniUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.uinsgd.ac.id/index.php/biodjati/oai, pertama di-harvest 7 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Genetic Profiling of Sida rhombifolia Originated from Several Indonesian Ethnicities Based on Sequence-Related Amplified Polymorphism Markers</dc:title>
	<dc:creator>Solihah, Jumailatus</dc:creator>
	<dc:creator>Kurniatanty, Isma</dc:creator>
	<dc:creator>Subositi, Dyah</dc:creator>
	<dc:creator>Maruzy, Anshary</dc:creator>
	<dc:creator>Martiwi, Ika Nugraheny Ari</dc:creator>
	<dc:creator>Ainy, Erny Qurrotul</dc:creator>
	<dc:creator>Anam, Khoirul</dc:creator>
	<dc:creator>Dina, Aslikh Lana</dc:creator>
	<dc:subject xml:lang="en-US">enetic characterisation</dc:subject>
	<dc:subject xml:lang="en-US">Sida rhombifolia</dc:subject>
	<dc:subject xml:lang="en-US">SRAP</dc:subject>
	<dc:description xml:lang="en-US">Sida rhombifolia is one of wild flowering plants that grows easily in many habitats with moderate humidity, with some usefulness in traditional medicine. Genetic characterization of Sida rhombifolia accessions originated from 12 ethnicities of Indonesia was analyzed based on Sequence-Related Amplified Polymorphism (SRAP) Markers. The genomic DNA were extracted from leaf samples and then were characterized by using the SRAP marker system according to Li and Quiros (2001). Nine pairs of SRAP primer resulted high polymorphic bands and were used in the genetic profiling. The data analysis was performed using GenAlEx to calculate genetic distance, Principal coordinate analysis, and Analysis of Molecular Variance (AMOVA), also using POPGENE to assess genetic diversity (Hs and Ht) and Nm to predict gene flow among populations. The coordinate analysis showed that the accessions originated from ethnicities along Wallacean line tend to differ genetically from most other locations. However, the results of analysis of molecular variance suggested that there were only slight differences (0.1%) found between ethnicities, while most genetic variances (99.9%) were found mostly among accessions within populations. The results suggested that there was an extensive genetic flow and plant spreading among Sida rhombifolia plant populations, resulting more homogenous genetic characters among most populations, while high diversity within population. The calculation of the number of migration (Nm = 1.7341) confirmed that the high rate of gene flow had occurred between populations.</dc:description>
	<dc:publisher xml:lang="en-US">UIN Sunan Gunung Djati Bandung</dc:publisher>
	<dc:date>2021-11-29</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.uinsgd.ac.id/index.php/biodjati/article/view/14553</dc:identifier>
	<dc:identifier>10.15575/biodjati.v6i2.14553</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Biodjati; Vol. 6 No. 2 (2021): November; 203-212</dc:source>
	<dc:source xml:lang="id-ID">Jurnal Biodjati; Vol 6 No 2 (2021): November; 203-212</dc:source>
	<dc:source>2541-4208</dc:source>
	<dc:source>2548-1606</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.uinsgd.ac.id/index.php/biodjati/article/view/14553/6351</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2021 Jurnal Biodjati</dc:rights>
</oai_dc:dc>