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Protease and Amylase Activities of Javaen barb (Systomus rubripinnis Val.)

Jurnal Biodjati · Vol. 7 No. 1 (2022) · hlm. 45–55

DOI
10.15575/biodjati.v7i1.15535
ISSN
2541-4208, 2548-1606
Terbit
2022-05-30
Penerbit
UIN Sunan Gunung Djati Bandung
Bahasa
eng

Abstrak

Studies on morphology, growth, and reproduction have been carried out on wild Javaen barb, but there was no information on its digestive capacity; therefore, the research was conducted to determine protease and amylase activities in the digestive tract. This study used a total of 50 barbs with body weights between 13.56 -128.93g / fish. The measurement of enzyme activity was carried out using the spectrophotometer method. The results showed that differences in fish size resulted in differences in protease activity, but not for amylase. Fish with a small size have a higher protease activity than fish with a larger size. The protease activity did not differ between pH 6.9 to 10.0 but was higher than pH 12.5. Protease activity also did not vary between the anterior and posterior intestine and between 30-50°C. Amylase activity also found no difference between the anterior and posterior intestine, but there was a difference in activity between temperatures of 30-50°C. In conclusion, protease activity occurs in a neutral to alkaline environment, and there were differences in protease activity between different body sizes but not between intestinal segments. Amylase activity occurs throughout the intestine and decreases at temperatures of 50°C.

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27 penulis · 100 tautan kepenulisan bersama · 41 lintas organisasi

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  • Jenderal Soedirman University
  • National Research and Innovation Agency
  • Tsinghua University
  • University of Bahri
  • Southeast Asian Fisheries Development Center
  • Nasional University
  • IPB University
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
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Rekan penulisOrganisasiArtikel bersama
Atang AtangJenderal Soedirman University7
Hana HanaJenderal Soedirman University7
Yulia SistinaJenderal Soedirman University5
Phuping SucharitakulTsinghua University5
Eko SetiyonoJenderal Soedirman University5
Sorta Basar Ida SimanjuntakJenderal Soedirman University3
Hanan Hassan Alsheikh MahmoudUniversity of Bahri3
Joko PamungkasNational Research and Innovation Agency3
Mellya Rizki PitrianiJenderal Soedirman University3
Ristiandani R. PradhyaningrumJenderal Soedirman University3
Koni OkthalinaJenderal Soedirman University3
Muthiara N. A. MulyaniJenderal Soedirman University3
I G A Ayu Ratna PuspitasariAfiliasi tidak diketahui2
Endah Sri PalupiJenderal Soedirman University2
Frolan A. AyaSoutheast Asian Fisheries Development Center2
Joko PamungkasNational Research and Innovation Agency2
Yuni ApriyantiNational Research and Innovation Agency2
Asrul Sahri SiregarJenderal Soedirman University1
Purnama SukardiJenderal Soedirman University1
Edy YuwonoNasional University1
Ridwan AffandiIPB University1
Havidh Ardika SyahputraJenderal Soedirman University1
Imelda ImeldaJenderal Soedirman University1
Siti WinasisJenderal Soedirman University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.uinsgd.ac.id/index.php/biodjati/oai, pertama di-harvest 7 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Protease and Amylase Activities of Javaen barb (Systomus rubripinnis Val.)</dc:title>
	<dc:creator>Susilo, Untung</dc:creator>
	<dc:creator>Rachmawati, Farida Nur</dc:creator>
	<dc:creator>Wibowo, Eko Setio</dc:creator>
	<dc:subject xml:lang="en-US">digestive enzyme</dc:subject>
	<dc:subject xml:lang="en-US">gut segment</dc:subject>
	<dc:subject xml:lang="en-US">Javaen barb</dc:subject>
	<dc:subject xml:lang="en-US">pH</dc:subject>
	<dc:subject xml:lang="en-US">temperature Hassk</dc:subject>
	<dc:description xml:lang="en-US">Studies on morphology, growth, and reproduction have been carried out on wild Javaen barb, but there was no information on its digestive capacity; therefore, the research was conducted to determine protease and amylase activities in the digestive tract. This study used a total of 50 barbs with body weights between 13.56 -128.93g / fish. The measurement of enzyme activity was carried out using the spectrophotometer method. The results showed that differences in fish size resulted in differences in protease activity, but not for amylase. Fish with a small size have a higher protease activity than fish with a larger size. The protease activity did not differ between pH 6.9 to 10.0 but was higher than pH 12.5. Protease activity also did not vary between the anterior and posterior intestine and between 30-50°C. Amylase activity also found no difference between the anterior and posterior intestine, but there was a difference in activity between temperatures of 30-50°C. In conclusion, protease activity occurs in a neutral to alkaline environment, and there were differences in protease activity between different body sizes but not between intestinal segments. Amylase activity occurs throughout the intestine and decreases at temperatures of 50°C.</dc:description>
	<dc:publisher xml:lang="en-US">UIN Sunan Gunung Djati Bandung</dc:publisher>
	<dc:date>2022-05-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.uinsgd.ac.id/index.php/biodjati/article/view/15535</dc:identifier>
	<dc:identifier>10.15575/biodjati.v7i1.15535</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Biodjati; Vol. 7 No. 1 (2022): May; 45-55</dc:source>
	<dc:source xml:lang="id-ID">Jurnal Biodjati; Vol 7 No 1 (2022): May; 45-55</dc:source>
	<dc:source>2541-4208</dc:source>
	<dc:source>2548-1606</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.uinsgd.ac.id/index.php/biodjati/article/view/15535/7122</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2022 Jurnal Biodjati</dc:rights>
</oai_dc:dc>