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An Extracellular Pectinase from ISH16 Bacteria Isolated Induced by Coffee Pulp Waste Substrate

Jurnal Biodjati · Vol. 7 No. 2 (2022) · hlm. 177–188

DOI
10.15575/biodjati.v7i2.20279
ISSN
2541-4208, 2548-1606
Terbit
2022-11-30
Penerbit
UIN Sunan Gunung Djati Bandung
Bahasa
eng

Abstrak

An α-1,4-glycosidic bonds galactoses pectin, mainly composed of a D-galacturonic acid chain, are important biomaterial widely used in industries. Utilizing this material, a bioprocess, including the biocatalysis pectinase, is often needed. Pectinase production was optimized in 7 days SSF at 37°C, and the pectinase activities were daily measured by the method of Somogy-Nelson. The optimum pectinase production was 0.166 U/ml on the fourth day SSF. Purification using open column ion exchange chromatography DEAE cellulose DE-52 resulted in 1030.9 folds of pectinase purity with a yield of 25.9%. The enzyme was at optimal activity at pH six and attended stable in the pH range of 5.5-8, while optimal activity at a temperature of 50°C and was stable in the range of 30-45°C. The pectinase activity increased by 120% with the addition of 10 mM Mg2+, and 95% retained when 10 mM Ca2+ was added. The presence of 10 mM Na+, K+, and Fe2+ resulted in a slight effect of activity at 85%, 83%, and 78%. However, it was strongly inhibited by 10 mM Al3+ and retained 25%. Based on the results above, the microbial utilization of coffee pulp waste by ISH16 bacteria pectinolytic is one opportunity to produce valuable pectinase with low-cost production, so comprehensive examination in large-scale production is needed too. In this paper, all research detail steps were described.

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  • Universitas Jember
  • Institut Teknologi dan Sains Mandala
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Rekan penulisOrganisasiArtikel bersama
Sutoyo SutoyoUniversitas Jember2
Rosita Fitrah DewiAfiliasi tidak diketahui1
Ahmad Bukhari SaragihUniversitas Jember1
Siswoyo SiswoyoUniversitas Jember1
Achmad MaududieUniversitas Jember1
Ferry WirantoInstitut Teknologi dan Sains Mandala1
Muhamat Abdul RohimInstitut Teknologi dan Sains Mandala1
Riza Bahtiar SulistyanInstitut Teknologi dan Sains Mandala1
Yuliatin AzizahInstitut Teknologi dan Sains Mandala1
Saiful AminInstitut Teknologi dan Sains Mandala1
Nurshadrina Kartika SariInstitut Teknologi dan Sains Mandala1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.uinsgd.ac.id/index.php/biodjati/oai, pertama di-harvest 7 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">An Extracellular Pectinase from ISH16 Bacteria Isolated Induced by Coffee Pulp Waste Substrate</dc:title>
	<dc:creator>Muzakhar, Kahar</dc:creator>
	<dc:creator>Elida, Farah Salma</dc:creator>
	<dc:creator>Putrasetya, Ramdhan</dc:creator>
	<dc:creator>Siswoyo, Siswoyo</dc:creator>
	<dc:creator>Winarsa, Rudju</dc:creator>
	<dc:creator>Wiyono, Hidayat Teguh</dc:creator>
	<dc:subject xml:lang="en-US">coffee pulp</dc:subject>
	<dc:subject xml:lang="en-US">ISH16 bacteria isolate</dc:subject>
	<dc:subject xml:lang="en-US">pectinase</dc:subject>
	<dc:subject xml:lang="en-US">purification</dc:subject>
	<dc:subject xml:lang="en-US">solid state fermentation</dc:subject>
	<dc:description xml:lang="en-US">An α-1,4-glycosidic bonds galactoses pectin, mainly composed of a D-galacturonic acid chain, are important biomaterial widely used in industries. Utilizing this material, a bioprocess, including the biocatalysis pectinase, is often needed. Pectinase production was optimized in 7 days SSF at 37°C, and the pectinase activities were daily measured by the method of Somogy-Nelson. The optimum pectinase production was 0.166 U/ml on the fourth day SSF. Purification using open column ion exchange chromatography DEAE cellulose DE-52 resulted in 1030.9 folds of pectinase purity with a yield of 25.9%. The enzyme was at optimal activity at pH six and attended stable in the pH range of 5.5-8, while optimal activity at a temperature of 50°C and was stable in the range of 30-45°C. The pectinase activity increased by 120% with the addition of 10 mM Mg2+, and 95% retained when 10 mM Ca2+ was added. The presence of 10 mM Na+, K+, and Fe2+ resulted in a slight effect of activity at 85%, 83%, and 78%. However, it was strongly inhibited by 10 mM Al3+ and retained 25%. Based on the results above, the microbial utilization of coffee pulp waste by ISH16 bacteria pectinolytic is one opportunity to produce valuable pectinase with low-cost production, so comprehensive examination in large-scale production is needed too. In this paper, all research detail steps were described.</dc:description>
	<dc:publisher xml:lang="en-US">UIN Sunan Gunung Djati Bandung</dc:publisher>
	<dc:date>2022-11-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.uinsgd.ac.id/index.php/biodjati/article/view/20279</dc:identifier>
	<dc:identifier>10.15575/biodjati.v7i2.20279</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Biodjati; Vol. 7 No. 2 (2022): November; 177-188</dc:source>
	<dc:source xml:lang="id-ID">Jurnal Biodjati; Vol 7 No 2 (2022): November; 177-188</dc:source>
	<dc:source>2541-4208</dc:source>
	<dc:source>2548-1606</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.uinsgd.ac.id/index.php/biodjati/article/view/20279/7858</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2022 Jurnal Biodjati</dc:rights>
</oai_dc:dc>