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Weissella paramesenteroides: A Lactic Acid Bacteria Producing Glutathione from Fermented Vegetable Based Keciwis Leaf

Jurnal Biodjati · Vol. 10 No. 1 (2025) · hlm. 55–65

DOI
10.15575/biodjati.v10i1.30589
ISSN
2541-4208, 2548-1606
Terbit
2025-05-31
Penerbit
UIN Sunan Gunung Djati Bandung
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc-nd/4.0

Abstrak

Glutathione (GSH), is one of the essential antioxidants to prevent free radicals and support the immune system. The low level of glutathione is associated with some diseases such as cancer, Alzheimer's, Parkinson's, and AIDS. Lactic acid bacteria (LAB) can produce glutathione. Indonesia, as a mega biodiversity country, has various vegetables and LAB sources that remain underexplored. This research aims to explore LAB-producing glutathione from fermented products derived from Indonesia's typical plant, the keciwis leaf. The method of this research includes sample preparation, lactic acid bacteria isolation, secondary metabolite production with cysteine precursor addition, glutathione analysis by Ellman's assay, identification of 16S rRNA gene of lactic acid bacteria, and glutathione qualitative analysis by HPLC. Fermented keciwis leaf-based products contain lactic acid bacteria with positive gram characteristics, can produce 0.33 – 0.37 mM extracellular glutathione. Precursor cysteine addition significantly increases glutathione (p<0,05). The selective lactic acid bacteria producing glutathione was identified as belonging to the genus Weissella, with a similarity value of 98.50%, closely related to Weissella paramesenteroides. Extracellular glutathione produced by this culture showed qualitative results on HPLC with a retention time of 6.34 minutes, indicating the presence of the sulfihydryl group. This study identified a new Indonesia source of lactic acid bacteria of Indonesian–origin lactic acid bacteria, specifically Weissella paramesenteroides, as a promising source for glutathione production

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  • Universitas Muhammadiyah Bandung
  • Universitas Raharja
  • Islamic University of Indonesia
  • Gunadarma University
  • IPB University
  • Warsaw University of Life Sciences
  • University of Agriculture Faisalabad
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Rekan penulisOrganisasiArtikel bersama
ErnastutiGunadarma University1
Ravi Ahmad SalimGunadarma University1
HARYANTO HARYANTOUniversitas Muhammadiyah Bandung1
Agus SutandiUniversitas Muhammadiyah Bandung1
Cusiyani AprilliaUniversitas Muhammadiyah Bandung1
Saepul Muhyi AmirullahUniversitas Muhammadiyah Bandung1
Faisal Anggi PraditaWarsaw University of Life Sciences1
Sugeng WidadaUniversitas Raharja1
Siti Patimah MaesarohUniversitas Raharja1
Siti Nurindah SariUniversitas Raharja1
Muhammad Rehan AnwarUniversity of Agriculture Faisalabad1
Ninda LutfianiUniversitas Raharja1
FernandoAfiliasi tidak diketahui1
Dwi JulianingsihUniversitas Raharja1
Bambang Purwoko Kusumo BintoroBakrie University1
Achmad ChafidzIslamic University of Indonesia1
Agam Duma Kalista WibowoInstitut Teknologi Indonesia1
Suharno RusdiIslamic University of Indonesia1
Imam NurrahmanIslamic University of Indonesia1
Adi KusmayadiNational Yunlin University of Science and Technology1
Danang Tri HartantoUniversitas Gadjah Mada1
Taufik Ramdani TohariPadjadjaran University1
Qori Atur Rodiah SuhadaUniversitas Muhammadiyah Bandung1
Tiara ZahiraUniversitas Muhammadiyah Bandung1
Nadila Suci NugrahaUniversitas Muhammadiyah Bandung1
Suryo WiyonoIPB University1
Tri Asmira DamayantiIPB University1
Abu BakarUIN Sunan Ampel Surabaya1
Siti Mauliana HairiniAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.uinsgd.ac.id/index.php/biodjati/oai, pertama di-harvest 7 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Weissella paramesenteroides: A Lactic Acid Bacteria Producing Glutathione  from Fermented Vegetable Based Keciwis Leaf</dc:title>
	<dc:creator>Luthfia Hastiani Muharram</dc:creator>
	<dc:creator>Haryanto</dc:creator>
	<dc:creator>Wulan Pertiwi</dc:creator>
	<dc:creator>Nelis Hernahadini</dc:creator>
	<dc:creator>Ima Mukaromah</dc:creator>
	<dc:subject xml:lang="en-US">glutathione </dc:subject>
	<dc:subject xml:lang="en-US">keciwis </dc:subject>
	<dc:subject xml:lang="en-US">lactic acid bacteria </dc:subject>
	<dc:subject xml:lang="en-US">weissella </dc:subject>
	<dc:description xml:lang="en-US">Glutathione (GSH), is one of the essential antioxidants to prevent free radicals and support the immune system. The low level of glutathione is associated with some diseases such as cancer, Alzheimer's, Parkinson's, and AIDS. Lactic acid bacteria (LAB) can produce glutathione. Indonesia, as a mega biodiversity country, has various vegetables and LAB sources that remain underexplored. This research aims to explore LAB-producing glutathione from fermented products derived from Indonesia's typical plant, the keciwis leaf. The method of this research includes sample preparation, lactic acid bacteria isolation, secondary metabolite production with cysteine precursor addition, glutathione analysis by Ellman's assay, identification of 16S rRNA gene of lactic acid bacteria, and glutathione qualitative analysis by HPLC.  Fermented keciwis leaf-based products contain lactic acid bacteria with positive gram characteristics, can produce 0.33 – 0.37 mM extracellular glutathione. Precursor cysteine addition significantly increases glutathione (p&amp;lt;0,05). The selective lactic acid bacteria producing glutathione was identified as belonging to the genus Weissella, with a similarity value of 98.50%, closely related to Weissella paramesenteroides. Extracellular glutathione produced by this culture showed qualitative results on HPLC with a retention time of 6.34 minutes, indicating the presence of the sulfihydryl group. This study identified a new Indonesia source of lactic acid bacteria of Indonesian–origin lactic acid bacteria, specifically Weissella paramesenteroides, as a promising source for glutathione production</dc:description>
	<dc:publisher xml:lang="en-US">UIN Sunan Gunung Djati Bandung</dc:publisher>
	<dc:date>2025-05-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.uinsgd.ac.id/index.php/biodjati/article/view/30589</dc:identifier>
	<dc:identifier>10.15575/biodjati.v10i1.30589</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Biodjati; Vol. 10 No. 1 (2025): May; 55-65</dc:source>
	<dc:source xml:lang="id-ID">Jurnal Biodjati; Vol 10 No 1 (2025): May; 55-65</dc:source>
	<dc:source>2541-4208</dc:source>
	<dc:source>2548-1606</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.uinsgd.ac.id/index.php/biodjati/article/view/30589/14258</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2025 Jurnal Biodjati</dc:rights>
	<dc:rights xml:lang="en-US">https://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
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