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Analysis of the Molecular Structure of Lipase-Dependent Chaperone from Ralstonia pickettii Strain BK6

Jurnal Biodjati · Vol. 9 No. 2 (2024) · hlm. 266–279

DOI
10.15575/biodjati.v9i2.33770
ISSN
2541-4208, 2548-1606
Terbit
2024-11-30
Penerbit
UIN Sunan Gunung Djati Bandung
Bahasa
eng

Abstrak

Several biotechnology industries are exploring the characteristics of lipase-dependent chaperones due to their distinctive biochemical traits. This study aimed to employ bioinformatics to analyze the molecular structure of Ralstonia picketii BK6's lipase-dependent chaperon (LipRM). The sequence mapping and amino acid distribution were examined using BioEdit (version 7.0.9.1). SignalP 5.0 and Interpro are employed for signal peptide detection, whereas Swiss-Model and VMD 1.9.2 are used for molecular dynamics modelling. The results showed that the Shine-Dalgarno sequence was discovered in the LipRM promoter, seven nucleotides upstream of the initiation codon (AUG) with the 5'-AGGAGA-3', and has a terminator region that facilitates the formation of a secondary structure. The protein's 3D structure prediction results indicate differences in the alpha helix chains (residues 166-174 and 254-271) between LipRM and the reference lipase. LipRM's molecular structure comprises a detachable signal peptide, and with variations in helix alpha chain conformation and ligand geometry.

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  • Universitas Halu Oleo
  • IPB University
  • Sriwijaya University
  • Universitas Sembilanbelas November Kolaka
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
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Rekan penulisOrganisasiArtikel bersama
Wa IbaUniversitas Halu Oleo2
Dirvamena BoerUniversitas Halu Oleo2
Nur Arfa YantiUniversitas Halu Oleo2
Wa Ode Linda Rahayu AnggrainiUniversitas Halu Oleo1
Nur Arfa YantiAfiliasi tidak diketahui1
Darwis SulemanUniversitas Halu Oleo1
Asrul SaniAfiliasi tidak diketahui1
Fahyuddin FahyuddinAfiliasi tidak diketahui1
Wa SintaAfiliasi tidak diketahui1
Haidin HaidinUniversitas Halu Oleo1
Indrawati .Afiliasi tidak diketahui1
Hasmiah .Afiliasi tidak diketahui1
Sitti Wirdhana AhmadAfiliasi tidak diketahui1
Andi Asrul AmirAfiliasi tidak diketahui1
Indrawati IndrawatiSriwijaya University1
Harmonika NyiliantriUniversitas Halu Oleo1
Wa Ode IsraAfiliasi tidak diketahui1
Vidya Nur Riska ParakkasiAfiliasi tidak diketahui1
Ruslan MajidUniversitas Halu Oleo1
Asrul SaniUniversitas Halu Oleo1
Fahmiati FahmiatiUniversitas Halu Oleo1
Ida UsmanUniversitas Halu Oleo1
Mukhsar MukhsarUniversitas Halu Oleo1
La GubuUniversitas Halu Oleo1
I Putu SudayasaAfiliasi tidak diketahui1
warhamni warhamniAfiliasi tidak diketahui1
Nisa Rachmania MubarikIPB University1
Antonius SuwantoIPB University1
Esti PuspitasariIPB University1
Andreas Adhi SatyaAfiliasi tidak diketahui1
Indrayani IndrayaniUniversitas Halu Oleo1
Indriyani NurUniversitas Halu Oleo1
Arman PariakanUniversitas Sembilanbelas November Kolaka1
Nurhayu MalikAfiliasi tidak diketahui1
Nyoman AdriniAfiliasi tidak diketahui1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://journal.uinsgd.ac.id/index.php/biodjati/oai, pertama di-harvest 7 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Analysis of the Molecular Structure of Lipase-Dependent Chaperone from Ralstonia pickettii Strain BK6</dc:title>
	<dc:creator>Azwar Syah, Muhamad</dc:creator>
	<dc:creator>Ambardini, Sri</dc:creator>
	<dc:creator>Jamili, Jamili</dc:creator>
	<dc:creator>Muzuni, Muzuni</dc:creator>
	<dc:creator>Trisandy, Darul</dc:creator>
	<dc:subject xml:lang="en-US">bioinformatics</dc:subject>
	<dc:subject xml:lang="en-US">lipase-dependent chaperone</dc:subject>
	<dc:subject xml:lang="en-US">modelling</dc:subject>
	<dc:description xml:lang="en-US">Several biotechnology industries are exploring the characteristics of lipase-dependent chaperones due to their distinctive biochemical traits. This study aimed to employ bioinformatics to analyze the molecular structure of Ralstonia picketii BK6's lipase-dependent chaperon (LipRM). The sequence mapping and amino acid distribution were examined using BioEdit (version 7.0.9.1). SignalP 5.0 and Interpro are employed for signal peptide detection, whereas Swiss-Model and VMD 1.9.2 are used for molecular dynamics modelling. The results showed that the Shine-Dalgarno sequence was discovered in the LipRM promoter, seven nucleotides upstream of the initiation codon (AUG) with the 5'-AGGAGA-3', and has a terminator region that facilitates the formation of a secondary structure. The protein's 3D structure prediction results indicate differences in the alpha helix chains (residues 166-174 and 254-271) between LipRM and the reference lipase. LipRM's molecular structure comprises a detachable signal peptide, and with variations in helix alpha chain conformation and ligand geometry.</dc:description>
	<dc:publisher xml:lang="en-US">UIN Sunan Gunung Djati Bandung</dc:publisher>
	<dc:date>2024-11-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.uinsgd.ac.id/index.php/biodjati/article/view/33770</dc:identifier>
	<dc:identifier>10.15575/biodjati.v9i2.33770</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Biodjati; Vol. 9 No. 2 (2024): November; 266-279</dc:source>
	<dc:source xml:lang="id-ID">Jurnal Biodjati; Vol 9 No 2 (2024): November; 266-279</dc:source>
	<dc:source>2541-4208</dc:source>
	<dc:source>2548-1606</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.uinsgd.ac.id/index.php/biodjati/article/view/33770/11894</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2024 Jurnal Biodjati</dc:rights>
</oai_dc:dc>