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End Point Polymerase Chain Reaction for Porcine Detection on Food Product of UIN Sunan Ampel Surabaya Canteen

Indonesian Journal of Halal Research · Vol. 3 No. 1 (2021) · hlm. 21–26

DOI
10.15575/ijhar.v3i1.11149
ISSN
2657-0165, 2656-3754
Terbit
2021-02-28
Penerbit
UIN Sunan Gunung Djati Bandung
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Halal food means food that permitted under Islamic law and fulfills about requirements. The absence of information about halal food contained in UIN Sunan Ampel Surabaya (UINSA) campus area causes related research to be carried out. This study aims to determine the porcine DNA contamination on food around UINSA area using molecular technology. Twenty two samples used were foods that contain meat and may contain pork obtained from canteens around UINSA area, analyzed using Polymerase Chain Reaction (PCR) method. The analysis was started with DNA isolation of 22 food samples, electrophoresis, PCR, then visualization gel electrophoresis. Primer gene coding for cytochrome b (cyt b) which produces 149 bp of DNA fragments. The results showed that no porcine contamination in 22 food samples, while the positive control showed a band of 149 bp. End point PCR method potentially to detect porcine DNA contaminants in food products around UINSA. Therefore the food is halal and safe for consumption.

Kata kunci

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14 penulis · 35 tautan kepenulisan bersama · 11 lintas organisasi

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  • UIN Sunan Ampel Surabaya
  • State University of Medan
  • State University of Makassar
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Miftahul Lathif AdzakiyyiUIN Sunan Ampel Surabaya2
Tri SusilowatiUIN Sunan Ampel Surabaya2
Hermalya Putri HartonoAfiliasi tidak diketahui1
Hanik FaizahUIN Sunan Ampel Surabaya1
Fauziyah HarahapState University of Medan1
Said Iskandar Al IdrusState University of Medan1
Puji PrastowoState University of Medan1
Eko PrasetyaState University of Medan1
Lazuardi LazuardiState University of Medan1
Yusnaeni YusufState University of Makassar1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://journal.uinsgd.ac.id/index.php/ijhar/oai, pertama di-harvest 7 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">End Point Polymerase Chain Reaction for Porcine Detection on Food Product of UIN Sunan Ampel Surabaya Canteen</dc:title>
	<dc:creator>Rokhim, Saiku</dc:creator>
	<dc:creator>Tyautari, Inggrit</dc:creator>
	<dc:creator>Firmansyah, M. Aliffiyan</dc:creator>
	<dc:creator>Rachmawati, Yuanita</dc:creator>
	<dc:subject xml:lang="en-US">end point PCR</dc:subject>
	<dc:subject xml:lang="en-US">pig cyt b gene</dc:subject>
	<dc:subject xml:lang="en-US">porcine detection</dc:subject>
	<dc:subject xml:lang="en-US">UIN Sunan Ampel Surabaya</dc:subject>
	<dc:description xml:lang="en-US">Halal food means food that permitted under Islamic law and fulfills about requirements. The absence of information about halal food contained in UIN Sunan Ampel Surabaya (UINSA) campus area causes related research to be carried out. This study aims to determine the porcine DNA contamination on food around UINSA area using molecular technology. Twenty two samples used were foods that contain meat and may contain pork obtained from canteens around UINSA area, analyzed using Polymerase Chain Reaction (PCR) method. The analysis was started with DNA isolation of 22 food samples, electrophoresis, PCR, then visualization gel electrophoresis. Primer gene coding for cytochrome b (cyt b) which produces 149 bp of DNA fragments. The results showed that no porcine contamination in 22 food samples, while the positive control showed a band of 149 bp. End point PCR method potentially to detect porcine DNA contaminants in food products around UINSA. Therefore the food is halal and safe for consumption.</dc:description>
	<dc:publisher xml:lang="en-US">UIN Sunan Gunung Djati Bandung</dc:publisher>
	<dc:date>2021-02-28</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.uinsgd.ac.id/index.php/ijhar/article/view/11149</dc:identifier>
	<dc:identifier>10.15575/ijhar.v3i1.11149</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Halal Research; Vol. 3 No. 1 (2021): February; 21-26</dc:source>
	<dc:source>2657-0165</dc:source>
	<dc:source>2656-3754</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.uinsgd.ac.id/index.php/ijhar/article/view/11149/5349</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2021 Indonesian Journal of Halal Research</dc:rights>
	<dc:rights xml:lang="en-US">https://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>