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Design and Performance Test of Specific Primers to Detect Bovine DNA Fragments using Multiplex PCR Technique for Halal Authentification

Indonesian Journal of Halal Research · Vol. 4 No. 2 (2022) · hlm. 45–52

DOI
10.15575/ijhar.v4i2.15573
ISSN
2657-0165, 2656-3754
Terbit
2022-08-31
Penerbit
UIN Sunan Gunung Djati Bandung
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Adulterating meat products with several species, including non-halal species, is often found in commercial products. This study aims to design and validate the Cytochrome c oxidase subunit I (CO1) primers to detect the non-halal species. A pair of species-specific primers encoding the CO1 gene were designed to amplify bovine DNA, tested for specificity, and applied in multiplex polymerase chain reaction (PCR) technique with D-loop primers for pigs, Cyt-b for rats, and 12S rRNA for dogs. The CO1 primers, along with D-loop primers for porcine, Cyt-b primers for rats, and 12S rRNA primers for dogs can be used to detect specific bovine DNA with a size of 279 bp and sequence similarity of 96%. In addition, dog, rat, and porcine DNA were amplified at 101, 603, and 951 bp, respectively. These four primers are specific and can amplify the target DNA to detect non-halal meat component contamination in a single reaction process.

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  • University of Brawijaya
  • Kyungpook National University
  • Hasanuddin University
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Rekan penulisOrganisasiArtikel bersama
Hasria AlangHasanuddin University1
Try ArdyatiAfiliasi tidak diketahui1
Suharjono SuharjonoUniversity of Brawijaya1
Andy SoegiantoAfiliasi tidak diketahui1
Wahyu WidoretnoUniversity of Brawijaya1
Nur BasukiAfiliasi tidak diketahui1
Dian SiswantoUniversity of Brawijaya1
Ainun Sayyidah ZakiyahUniversity of Brawijaya1
Rosina MonicaUniversity of Brawijaya1
Rodiyati AzrianingsihUniversity of Brawijaya1
Riza Nensy MarantikaUniversity of Brawijaya1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.uinsgd.ac.id/index.php/ijhar/oai, pertama di-harvest 7 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Design and Performance Test of Specific Primers to Detect Bovine DNA Fragments using Multiplex PCR Technique for Halal Authentification</dc:title>
	<dc:creator>Kusnadi, Joni</dc:creator>
	<dc:creator>Hernandi, Kevin Hohn</dc:creator>
	<dc:creator>Al-Awwaly, Khotibul Umam</dc:creator>
	<dc:creator>Arumingtyas, Estri Laras</dc:creator>
	<dc:creator>Hakiki, Hilda Maghfirotu</dc:creator>
	<dc:creator>Istianah, Nur</dc:creator>
	<dc:subject xml:lang="en-US">bovine</dc:subject>
	<dc:subject xml:lang="en-US">CO1 primer</dc:subject>
	<dc:subject xml:lang="en-US">halal authentification</dc:subject>
	<dc:subject xml:lang="en-US">meat products</dc:subject>
	<dc:subject xml:lang="en-US">multiplex PCR</dc:subject>
	<dc:description xml:lang="en-US">Adulterating meat products with several species, including non-halal species, is often found in commercial products. This study aims to design and validate the Cytochrome c oxidase subunit I (CO1) primers to detect the non-halal species. A pair of species-specific primers encoding the CO1 gene were designed to amplify bovine DNA, tested for specificity, and applied in multiplex polymerase chain reaction (PCR) technique with D-loop primers for pigs, Cyt-b for rats, and 12S rRNA for dogs. The CO1 primers, along with D-loop primers for porcine, Cyt-b primers for rats, and 12S rRNA primers for dogs can be used to detect specific bovine DNA with a size of 279 bp and sequence similarity of 96%. In addition, dog, rat, and porcine DNA were amplified at 101, 603, and 951 bp, respectively. These four primers are specific and can amplify the target DNA to detect non-halal meat component contamination in a single reaction process.</dc:description>
	<dc:publisher xml:lang="en-US">UIN Sunan Gunung Djati Bandung</dc:publisher>
	<dc:date>2022-08-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.uinsgd.ac.id/index.php/ijhar/article/view/15573</dc:identifier>
	<dc:identifier>10.15575/ijhar.v4i2.15573</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Halal Research; Vol. 4 No. 2 (2022): August; 45-52</dc:source>
	<dc:source>2657-0165</dc:source>
	<dc:source>2656-3754</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.uinsgd.ac.id/index.php/ijhar/article/view/15573/7622</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2022 Author</dc:rights>
	<dc:rights xml:lang="en-US">https://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>