Melola Discovery

← Melola Discovery

Jurnal SINTA 1

Consideration of the Cycle Threshold Values from Real-Time RT-PCR SARS-CoV-2 Interpretation for the Clinicians: Analysis of 339 Positive Cases from a Referral Laboratory in Jakarta, Indonesia

ACTA MEDICA INDONESIANA · Vol. 53 No. 1 (2021)

ISSN
2338-2732, 0125-9326
Terbit
2021-03-31
Penerbit
The Indonesian Society of Internal Medicine
Bahasa
eng

Abstrak

Background: real-time RT-PCR was recommended by WHO for COVID-19 diagnosis. The cycle threshold (Ct) values were expected to have an association with clinical manifestation. However, the diagnostic modalities such as quantitative molecular detection and virus isolation were not yet available for the routine test. This study has been conducted to analyze the relationship between the Ct values of qualitative rRT-PCR and the clinical manifestation and to describe the factors determining the result. Methods: from March to April 2020, specimens were sent to our laboratory from different healthcare centers in Jakarta. The patient’s characteristic and clinical manifestation were extracted from the specimen’s epidemiology forms. The specimens extracted and tested using rRT-PCR, and the Ct value were collected. The data were analyzed using the appropriate statistic test. Results: from 339 positive results, the mild to moderate case was 176 (52%) and the severe cases was 163 (48%). Female was dominant in the mild to moderate cases (58%), while the male was prevalent in the severe cases (60%). The median age for mild to moderate case was 35 years old and severe cases was 49 years old. Statistical analysis found relationship between both group with gender (p = 0.001) and age (p < 0.001), but not with the Ct value. Conclusion: many variables in specimen sampling and processing could affect the Ct value result. In addition, the disease’s severity was depended with the host immune response, regardless the number of virus. There was suggested no significant difference between the Ct values of mild-moderate and severe COVID-19, and thus should not be loosely interpreted.

Kata kunci

Jaringan kolaborasi para penulis

40 penulis · 173 tautan kepenulisan bersama · 1 lintas organisasi · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • Universitas Airlangga
  • Universitas Islam Negeri Ar-Raniry
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Dita AditianingsihAfiliasi tidak diketahui4
Rudyanto SedonoAfiliasi tidak diketahui2
Mardiastuti H WahidAfiliasi tidak diketahui2
Ari Fahrial SyamAfiliasi tidak diketahui2
Samsuridjal DjauziAfiliasi tidak diketahui2
Adityo SusiloAfiliasi tidak diketahui2
Dyah Wulan SariUniversitas Airlangga1
Gigih PrihantonoUniversitas Airlangga1
Wasiaturrahma WasiaturrahmaUniversitas Airlangga1
Maskur MaskurUniversitas Islam Negeri Ar-Raniry1
Purwantyastuti PurwantyastutiAfiliasi tidak diketahui1
Gestina AliskaAfiliasi tidak diketahui1
Rianto SetiabudiAfiliasi tidak diketahui1
Trisni U DewiAfiliasi tidak diketahui1
Muhammad K AzwarAfiliasi tidak diketahui1
Mursinah MursinahAfiliasi tidak diketahui1
Evy YunihastutiAfiliasi tidak diketahui1
Ardiana KusumaningrumAfiliasi tidak diketahui1
Budiman BelaAfiliasi tidak diketahui1
Rike SyahniarAfiliasi tidak diketahui1
Kuntjoro HarimurtiAfiliasi tidak diketahui1
Rino A GaniAfiliasi tidak diketahui1
Nafrialdi NafrialdiAfiliasi tidak diketahui1
Juferdy KurniawanAfiliasi tidak diketahui1
Suzanna ImannuelAfiliasi tidak diketahui1
Suhendro SuhendroAfiliasi tidak diketahui1
Ika P WijayaAfiliasi tidak diketahui1
Cleopas M RumendeAfiliasi tidak diketahui1
Cosphiadi IrawanAfiliasi tidak diketahui1
Ceva W PitoyoAfiliasi tidak diketahui1
Benny ZulkarnainAfiliasi tidak diketahui1
Nuri D IndrasariAfiliasi tidak diketahui1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) http://www.actamedindones.org/index.php/ijim/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Consideration of the Cycle Threshold Values from Real-Time RT-PCR SARS-CoV-2 Interpretation for the Clinicians: Analysis of 339 Positive Cases from a Referral Laboratory in Jakarta, Indonesia</dc:title>
	<dc:creator>Ibrahim, Fera</dc:creator>
	<dc:creator>natasha, Augustine</dc:creator>
	<dc:creator>Saharman, Yulia Rosa</dc:creator>
	<dc:creator>Yasmon, Andi</dc:creator>
	<dc:creator>Fithriyah, Fithriyah</dc:creator>
	<dc:creator>Karuniawati, Anis</dc:creator>
	<dc:creator>Ganiesa, Selvia</dc:creator>
	<dc:creator>Sudarmono, Pratiwi</dc:creator>
	<dc:subject xml:lang="en-US">SARS-Cov-2</dc:subject>
	<dc:subject xml:lang="en-US">Cycle Threshold</dc:subject>
	<dc:subject xml:lang="en-US">rRT-PCR</dc:subject>
	<dc:subject xml:lang="en-US">clinical interpretation</dc:subject>
	<dc:subject xml:lang="en-US">COVID 19</dc:subject>
	<dc:description xml:lang="en-US">Background: real-time RT-PCR was recommended by WHO for COVID-19 diagnosis. The cycle threshold (Ct) values were expected to have an association with clinical manifestation. However, the diagnostic modalities such as quantitative molecular detection and virus isolation were not yet available for the routine test. This study has been conducted to analyze the relationship between the Ct values of qualitative rRT-PCR and the clinical manifestation and to describe the factors determining the result. Methods: from March to April 2020, specimens were sent to our laboratory from different healthcare centers in Jakarta. The patient’s characteristic and clinical manifestation were extracted from the specimen’s epidemiology forms. The specimens extracted and tested using rRT-PCR, and the Ct value were collected. The data were analyzed using the appropriate statistic test. Results: from 339 positive results, the mild to moderate case was 176 (52%) and the severe cases was 163 (48%). Female was dominant in the mild to moderate cases (58%), while the male was prevalent in the severe cases (60%). The median age for mild to moderate case was 35 years old and severe cases was 49 years old. Statistical analysis found relationship between both group with gender (p = 0.001) and age (p &amp;lt; 0.001), but not with the Ct value. Conclusion: many variables in specimen sampling and processing could affect the Ct value result. In addition, the disease’s severity was depended with the host immune response, regardless the number of virus. There was suggested no significant difference between the Ct values of mild-moderate and severe COVID-19, and thus should not be loosely interpreted.</dc:description>
	<dc:publisher xml:lang="en-US">The Indonesian Society of Internal Medicine</dc:publisher>
	<dc:date>2021-03-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Cross sectional study</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://www.actamedindones.org/index.php/ijim/article/view/1599</dc:identifier>
	<dc:source xml:lang="en-US">Acta Medica Indonesiana; Vol. 53 No. 1 (2021): Acta Medica Indonesiana; 13</dc:source>
	<dc:source>2338-2732</dc:source>
	<dc:source>0125-9326</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://www.actamedindones.org/index.php/ijim/article/view/1599/pdf</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2021 Acta Medica Indonesiana</dc:rights>
</oai_dc:dc>