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Diversity of Ploidy Level and Morphology of Stevia rebaudiana Bertoni Induced by Colchicine

AGRIVITA, Journal of Agricultural Science · Vol. 48 No. 2 (2026) · hlm. 459–480

DOI
10.17503/agrivita.v48i2.5028
ISSN
2477-8516, 0126-0537
Terbit
2026-06-11
Penerbit
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

The constraints in the generative propagation of stevia include a limited supply of fertile seeds, suboptimal germination rates, and significant phenotypic variability, all of which hinder conventional breeding efforts. Vegetative propagation causes genetic uniformity but limits genetic diversity. Another method for breeding stevia is by inducing polyploidy with colchicine. The main goals of polyploidy induction are to enhance diversity, improve agronomic traits, and achieve ploidy higher than diploid. This study evaluated the ploidy levels and morphological responses of two accessions (SR16 and SR17) to five colchicine doses (0.05, 0.1, 0.15, and 0.2%) and three soaking periods (8, 16, and 24 hours). The accession SR16 showed the best response to 0.1% colchicine for 8 hours and to 0.15% for 12 hours, causing ploidy levels to shift from diploid to tetraploid and mixoploid. SR17, on the other hand, needed higher concentrations and longer immersion times (0.2% for 12 hours) to show the same range of variability. The changes in shape depended on the genotype. SR16 needed low to moderate levels of immersion for short periods, while SR17 needed high levels for long periods. These results endorse the utilization of polyploidy induction techniques in stevia breeding to enhance production and sweetness quality.

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  • Universitas Wahid Hasyim
  • State University of Malang
  • Bandung Institute of Technology
  • Universitas Islam Kadiri
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Rekan penulisOrganisasiArtikel bersama
Arifin Noor SugihartoUniversity of Brawijaya5
Devi MienantiUniversity of Brawijaya4
Iqbal HidayatAfiliasi tidak diketahui4
Yanuar DanaatmadjaAfiliasi tidak diketahui4
Mohammad Bahrelfi BelaffifAfiliasi tidak diketahui4
Ajay Goel KumarAfiliasi tidak diketahui4
Izmi YulianahUniversity of Brawijaya3
Gita Novita SariUniversity of Brawijaya3
Prakit SomtaKasetsart University2
Wiwin Sumiya Dwi YamikaUniversity of Brawijaya2
Rawina SaragihUniversity of Brawijaya2
Chindy Ulima ZanettaBandung Institute of Technology2
R. D. AndayaniUniversitas Islam Kadiri2
Nunun BarunawatiUniversity of Brawijaya2
Farida FaridaUniversity of Brawijaya2
Yuniel Melvanolo ZendratoSatya Wacana Christian University2
Dedy SupriadiUniversity of Brawijaya2
Yusuf Mufti BimantaraIPB University2
Eko WidaryantoUniversity of Brawijaya2
Nur Indah AgustinaUniversity of Brawijaya1
Effendy EffendyState University of Malang1
Respatijarti RespatijartiUniversity of Brawijaya1
Anna Satyana KaryawatiUniversity of Brawijaya1
Fakhri HafidhUniversity of Brawijaya1
Zainyah Salmah ArruumUniversity of Brawijaya1
Markus Miten KledenUniversity of Brawijaya1
Hendrawan SoetantoUniversity of Brawijaya1
Kusmartono KusmartonoUniversity of Brawijaya1
Suyadi SuyadiUniversity of Brawijaya1
Mudzakkir AliUniversitas Wahid Hasyim1
Heri KustantoUniversitas Wahid Hasyim1
Ahmad Alif Riyan MahdyUniversity of Brawijaya1
Rima RahmaUniversity of Brawijaya1
Peerasak SrinivesKasetsart University1
Nurul AiniUniversity of Brawijaya1
Agus Prayitno KurniawanUniversitas Trunodjoyo Madura1

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Sumber metadata: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Diversity of Ploidy Level and Morphology of Stevia rebaudiana Bertoni Induced by Colchicine</dc:title>
	<dc:creator>Ridhawati, Aprilia</dc:creator>
	<dc:creator>Saptadi, Darmawan</dc:creator>
	<dc:creator>Waluyo, Budi</dc:creator>
	<dc:creator>Kuswanto, Kuswanto</dc:creator>
	<dc:subject xml:lang="en-US">plant breeding</dc:subject>
	<dc:subject xml:lang="en-US">Colchicine; Morphological characteristics; Ploidy level; Polyploidy induction; Stevia rebaudiana Bertoni</dc:subject>
	<dc:description xml:lang="en-US">The constraints in the generative propagation of stevia include a limited supply of fertile seeds, suboptimal germination rates, and significant phenotypic variability, all of which hinder conventional breeding efforts. Vegetative propagation causes genetic uniformity but limits genetic diversity. Another method for breeding stevia is by inducing polyploidy with colchicine. The main goals of polyploidy induction are to enhance diversity, improve agronomic traits, and achieve ploidy higher than diploid. This study evaluated the ploidy levels and morphological responses of two accessions (SR16 and SR17) to five colchicine doses (0.05, 0.1, 0.15, and 0.2%) and three soaking periods (8, 16, and 24 hours). The accession SR16 showed the best response to 0.1% colchicine for 8 hours and to 0.15% for 12 hours, causing ploidy levels to shift from diploid to tetraploid and mixoploid. SR17, on the other hand, needed higher concentrations and longer immersion times (0.2% for 12 hours) to show the same range of variability. The changes in shape depended on the genotype. SR16 needed low to moderate levels of immersion for short periods, while SR17 needed high levels for long periods. These results endorse the utilization of polyploidy induction techniques in stevia breeding to enhance production and sweetness quality.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
	<dc:contributor xml:lang="en-US">Aprilia Ridhawati, Sweetener and Fiber Plant Assembly and Testing Center, Indonesian Ministry of Agriculture - Ministry o</dc:contributor>
	<dc:date>2026-06-11</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/5028</dc:identifier>
	<dc:identifier>10.17503/agrivita.v48i2.5028</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 48, No 2 (2026); 459-480</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v48i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/5028/2000</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/5028/3472</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2026 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>