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Exploration and Antifungal Assay of Endophytic Fungi as Biocontrol of Onion Purple Blotch Disease Caused by Alternaria porri (Ell) Cif In Vitro

AGRIVITA, Journal of Agricultural Science · Vol. 43 No. 1 (2020) · hlm. 114–124

DOI
10.17503/agrivita.v43i1.2838
ISSN
2477-8516, 0126-0537
Terbit
2020-12-30
Penerbit
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Purple blotch disease caused by Alternaria porri is the main destructive foliar disease of genus Allium, causing significant losses in yield of the crops. Recently, purple blotch disease is controlled by synthetic fungicides. However, fungicides have negative effects on the environment. Endophytic fungi can be used as an alternative control because a close symbiosis with the internal tissue of the host can minimize competition in new and complex ecosystems. This study aimed to explore and identify endophytic fungi that have the highest inhibition ability against A. porri and investigate the antagonistic mechanism. The method used in this study is an exploration of endophytic fungi, isolation of A. porri, in vitro antagonism tests, observation of the antagonistic mechanism, extraction of crude protein, SDS-PAGE, and identification. The antagonistic fungi that had the highest inhibition ability were identified as Penicillium citrinum with an inhibitory of 60.04%. Crude protein extracted from P. citrinum which showed inhibitory activity against A. porri is saturation level of ammonium sulfate 80% with a molecular weight of 40 kDa. This study implies that P. citrinum can inhibit the growth of A. porri through its anti fungi compounds. Further in vivo assays or field trials will need to be conducted in future studies.

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  • University of Brawijaya
  • National Pingtung University of Science and Technology
  • National Research and Innovation Agency
  • Universitas Muhammadiyah Malang
  • Universitas Jember
  • The University of Osaka
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  • Organisasi berbeda
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Rekan penulisOrganisasiArtikel bersama
Abdul Latief AbadiUniversity of Brawijaya5
Hagus TarnoUniversity of Brawijaya4
Eriyanto YusnawanNational Research and Innovation Agency3
Adi SetiawanUniversity of Brawijaya2
Nurul AiniUniversity of Brawijaya2
Wiwin Sumiya Dwi YamikaUniversity of Brawijaya2
Ito FernandoUniversity of Brawijaya2
Muhammad Akhid Syib’liUniversity of Brawijaya2
Irisa TriantiUniversity of Brawijaya2
Alfi InayatiUniversity of Brawijaya2
Mintarto MartosudiroUniversity of Brawijaya2
Restu Rizkyta KusumaNational Pingtung University of Science and Technology1
Chiu-Chsiung ChengNational Pingtung University of Science and Technology1
Yi‐Hsien LinNational Pingtung University of Science and Technology1
Yohana Avelia SandyNational Pingtung University of Science and Technology1
Yo-Chia ChenNational Pingtung University of Science and Technology1
Erfan Dani SeptiaUniversitas Muhammadiyah Malang1
Ardiyan TarunaUniversity of Brawijaya1
Rifani Rusiana DewiUniversity of Brawijaya1
Syarifah Maulidya RahmahUniversity of Brawijaya1
Bambang SugihartoUniversitas Jember1
Nur BasukiUniversity of Brawijaya1
Slameto SlametoAfiliasi tidak diketahui1
Muhammad Fariq Al HusniUniversity of Brawijaya1
Bayu Aji PamungkasUniversity of Brawijaya1
Sastia Prama PutriThe University of Osaka1
Syamsuddin DjauhariUniversity of Brawijaya1
Wiwit Wicaksono JatiUniversity of Brawijaya1
Rina RachmawatiUniversity of Brawijaya1
Theresia Rani Kartika AyuAfiliasi tidak diketahui1
Akhmad RizaliUniversity of Brawijaya1
Muhammad Irfan FebriansyahUniversity of Brawijaya1
Yogo SetiawanUniversity of Brawijaya1
Fibrianti Shinta DewiUniversity of Brawijaya1
Wiwin Sumiya Dwi YamikaUniversity of Brawijaya1
Nurul AiniUniversity of Brawijaya1
Puput WahyuningsihUniversity of Brawijaya1

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Sumber metadata: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc)
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Exploration and Antifungal Assay of Endophytic Fungi as Biocontrol of Onion Purple Blotch Disease Caused by Alternaria porri (Ell) Cif In Vitro</dc:title>
	<dc:creator>Firdausi, Wita</dc:creator>
	<dc:creator>Sulistyowati, Liliek</dc:creator>
	<dc:creator>Aini, Luqman Qurata</dc:creator>
	<dc:subject xml:lang="en-US">disease -plant scince</dc:subject>
	<dc:subject xml:lang="en-US">Allium ascalonicum; Antagonism; Antifungal activity; Biocontrol; Endophytic fungi</dc:subject>
	<dc:description xml:lang="en-US">Purple blotch disease caused by Alternaria porri is the main destructive foliar disease of genus Allium, causing significant losses in yield of the crops. Recently, purple blotch disease is controlled by synthetic fungicides. However, fungicides have negative effects on the environment. Endophytic fungi can be used as an alternative control because a close symbiosis with the internal tissue of the host can minimize competition in new and complex ecosystems. This study aimed to explore and identify endophytic fungi that have the highest inhibition ability against A. porri and investigate the antagonistic mechanism. The method used in this study is an exploration of endophytic fungi, isolation of A. porri, in vitro antagonism tests, observation of the antagonistic mechanism, extraction of crude protein, SDS-PAGE, and identification. The antagonistic fungi that had the highest inhibition ability were identified as Penicillium citrinum with an inhibitory of 60.04%. Crude protein extracted from P. citrinum which showed inhibitory activity against A. porri is saturation level of ammonium sulfate 80% with a molecular weight of 40 kDa. This study implies that P. citrinum can inhibit the growth of A. porri through its anti fungi compounds. Further in vivo assays or field trials will need to be conducted in future studies.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
	<dc:contributor xml:lang="en-US">Pest and Disease Department, Faculty of Agriculture, Brawijaya University</dc:contributor>
	<dc:date>2020-12-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/2838</dc:identifier>
	<dc:identifier>10.17503/agrivita.v43i1.2838</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 43, No 1 (2021); 114-124</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v1i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/2838/1351</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/2838/2145</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/2838/2146</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2021 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>