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Optimization of Somatic Embryogenesis Formation in Hoya (Hoya carnosa (L.) R. Br.) with Different Hormone Combinations

AGRIVITA, Journal of Agricultural Science · Vol. 48 No. 2 (2026) · hlm. 386–396

DOI
10.17503/agrivita.v41i0.4844
ISSN
2477-8516, 0126-0537
Terbit
2026-05-19
Penerbit
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Hoya carnosa (L.) R. Br. propagation is conventionally less efficient, and the amount produced is low. This study aims to determine the influence of hormone type and concentration on changes in cell structure and the phase of somatic embryogenesis of the Hoya plant in vitro. The study was conducted in two stages, namely the first stage of callus induction with a combination of BAP (0.4 mg/l, 0.5 mg/l, 0.6 mg/l) and 2,4-D (5 mg/l, 6 mg/l, 7 mg/l) and the second stage of somatic embryogenesis with a combination of BAP hormones (0.5 mg/l, 1.0 mg/l, 1.5 mg/l) and IBA (0.25 mg/l, 0.5 mg/l, and 0.75 mg/l). The data were analyzed using ANOVA and further analyzed by using DMRT. Based on the results, the combination of BAP 0.4 mg/l and 2,4-D 7 mg/l hormones showed the best growth with the parameters of the appearance of callus at 23 days, callus weight 2.92 g, and embryogenic calluses formed with crumb texture and greenish-yellow color. The combination of BAP 1 mg/l and IBA 0.75 mg/l resulted in the frequency of budding emergence of 23.3 days and the number of tendrils 20.7. This treatment was able to produce 5 roots and 2 shoots.

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  • Universitas Jember
  • University of Sarjanawiyata Tamansiswa
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Parawita DewantiAfiliasi tidak diketahui5
Ankardiansyah Pandu PradanaAfiliasi tidak diketahui4
Mohammad Nur KhozinAfiliasi tidak diketahui4
Sri HartatikAfiliasi tidak diketahui4
Mohammad HoesainAfiliasi tidak diketahui3
Tri HandoyoAfiliasi tidak diketahui3
Popy Hartatie HardjoAfiliasi tidak diketahui3
Eko Nur SuliswantoAfiliasi tidak diketahui3
Miswar MiswarAfiliasi tidak diketahui3
Anang SyamsuniharAfiliasi tidak diketahui3
Yusuf Dary AhnafAfiliasi tidak diketahui2
IryonoAfiliasi tidak diketahui2
Sri WahyuningsihAfiliasi tidak diketahui2
Abdul JalilUniversitas Jember1
Sri HartatikUniversitas Jember1
Rachmi MasnilahAfiliasi tidak diketahui1
Suhartiningsih Dwi NurchayantiAfiliasi tidak diketahui1
Dwi Tirta SariAfiliasi tidak diketahui1
Farchan Mushal Al RamadhanAfiliasi tidak diketahui1
RismayaniAfiliasi tidak diketahui1
Sri Ita TariganAfiliasi tidak diketahui1
Dewi Kusuma WardaniUniversity of Sarjanawiyata Tamansiswa1
Kyung-Min KimAfiliasi tidak diketahui1
Eviyanti ChoirunnisaAfiliasi tidak diketahui1
Agung Nugroho PuspitoAfiliasi tidak diketahui1
Mohammad UbaidillahAfiliasi tidak diketahui1
Tri Candra SetiawatiAfiliasi tidak diketahui1
Almansyah Nur SinatryaAfiliasi tidak diketahui1
Mohammad Nur KhozinAfiliasi tidak diketahui1
Wahyu Indra Duwi FanataAfiliasi tidak diketahui1
Bambang SugihartoAfiliasi tidak diketahui1
Galang Rizki RamadhanAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Optimization of Somatic Embryogenesis Formation in Hoya (Hoya carnosa (L.) R. Br.) with Different Hormone Combinations</dc:title>
	<dc:creator>Restanto, Didik Pudji</dc:creator>
	<dc:creator>Prayoga, Mohammad Candra</dc:creator>
	<dc:creator>Jaenuri, Ahmad</dc:creator>
	<dc:creator>Avivi, Sholeh</dc:creator>
	<dc:creator>Soeparjono, Sigit</dc:creator>
	<dc:creator>Selopa, Tanaya Asmara Citra</dc:creator>
	<dc:creator>Ullah, Rehan</dc:creator>
	<dc:creator>Alfarisy, Fariz Kustiawan</dc:creator>
	<dc:subject xml:lang="en-US">agronomy and horticulture</dc:subject>
	<dc:subject xml:lang="en-US">Histology; Hoya carnosa; Somatic Embryogenesis</dc:subject>
	<dc:description xml:lang="en-US">Hoya carnosa (L.) R. Br. propagation is conventionally less efficient, and the amount produced is low. This study aims to determine the influence of hormone type and concentration on changes in cell structure and the phase of somatic embryogenesis of the Hoya plant in vitro. The study was conducted in two stages, namely the first stage of callus induction with a combination of BAP (0.4 mg/l, 0.5 mg/l, 0.6 mg/l) and 2,4-D (5 mg/l, 6 mg/l, 7 mg/l) and the second stage of somatic embryogenesis with a combination of BAP hormones (0.5 mg/l, 1.0 mg/l, 1.5 mg/l) and IBA (0.25 mg/l, 0.5 mg/l, and 0.75 mg/l). The data were analyzed using ANOVA and further analyzed by using DMRT. Based on the results, the combination of BAP 0.4 mg/l and 2,4-D 7 mg/l hormones showed the best growth with the parameters of the appearance of callus at 23 days, callus weight 2.92 g, and embryogenic calluses formed with crumb texture and greenish-yellow color. The combination of BAP 1 mg/l and IBA 0.75 mg/l resulted in the frequency of budding emergence of 23.3 days and the number of tendrils 20.7. This treatment was able to produce 5 roots and 2 shoots.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2026-05-19</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/4844</dc:identifier>
	<dc:identifier>10.17503/agrivita.v41i0.4844</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 48, No 2 (2026); 386-396</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v48i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/4844/1981</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2026 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>