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Development of Simple-Sequence Repeats Markers from Durian (Durio zibethinus Murr. cultv. Matahari) Genomic Library

AGRIVITA, Journal of Agricultural Science · Vol. 39 No. 3 (2017) · hlm. 257–265

DOI
10.17503/agrivita.v39i3.1171
ISSN
2477-8516, 0126-0537
Terbit
2017-08-01
Penerbit
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Simple sequence repeats have been proved as powerful markers and widely used in molecular breeding to reduce cycles and cost efective. The availability of the marker is, however, very limited in durian. This research aimed to develop SSR markers from durian genomic library. Genomic DNA was isolated from durian shoot leaf, whilst SSR motifs were isolated using membrane-based oligonucleotide enrichment hybridization protocol. Annotation made on the library found 527 unique motifs from 354 durian libraries which form 425 loci. The SSR motifs obtained were generally short repeats which reached 89.6 %, whilst longer repeats were found consisted of compound motifs. Eleven loci were selected as representative for further test to prove their informativity. A number of unique allels were successfully amplified from 17 durian genomes. The analysis showed the polymorphic information content (PIC) values ranged from 0.000 to 0.662 with an average of 0.390. The SSR loci also showed their ability to be used for durian diversity analysis as the evident that the loci could be used as genetic markers for assisting further durian breeding program.

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  • Bandung Institute of Technology
  • Sultan Ageng Tirtayasa University
  • IPB University
  • Indonesia University of Education
  • Atma Jaya Catholic University of Indonesia
  • Telkom University
  • Universitas Islam Negeri Sultan Syarif Kasim Riau
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Rekan penulisOrganisasiArtikel bersama
Rizkita Rachmi EsyantiBandung Institute of Technology3
Indah Juwita SariSultan Ageng Tirtayasa University2
Topik HidayatIndonesia University of Education2
Veronica Sri Harjati SuhardiBandung Institute of Technology2
Sukrasno SukrasnoBandung Institute of Technology2
Muhandinni ZahraBandung Institute of Technology2
Listya Utami KarmawanAtma Jaya Catholic University of Indonesia2
Fenny Martha DwivanyBandung Institute of Technology2
Ayu PurwariantiBandung Institute of Technology1
Untari Novia WisestyTelkom University1
Tati Rajab MengkoBandung Institute of Technology1
Indria WahyuniSultan Ageng Tirtayasa University1
Rida Oktorida KhastiniSultan Ageng Tirtayasa University1
Ewi AwaliyatiSultan Ageng Tirtayasa University1
Andriana SusilowatiSultan Ageng Tirtayasa University1
Enggar UtariSultan Ageng Tirtayasa University1
Andre SpartaAfiliasi tidak diketahui1
Arifah RahmiAfiliasi tidak diketahui1
Ida FitrianingsihAfiliasi tidak diketahui1
Affandi AffandiAfiliasi tidak diketahui1
Dhasa LististioUniversitas Islam Negeri Sultan Syarif Kasim Riau1
Ellina MansyahAfiliasi tidak diketahui1
Dewi SartiamiIPB University1
Sudarsono SudarsonoIPB University1
Lalu Firman BudimanAfiliasi tidak diketahui1
Ardha ApriyantoAfiliasi tidak diketahui1
Diah KusumawatyIndonesia University of Education1
Fainmarinat Selviani InabuyUdayana University1
Udjianna Sekteria PasaribuBandung Institute of Technology1
Sapto Wahyu IndratnoBandung Institute of Technology1
Abdullah SonhajiBandung Institute of Technology1
Pingkan AditiawatiBandung Institute of Technology1
Meirina Kartika KusumawardhaniBandung Institute of Technology1
Utut WidyastutiIPB University1
Nampiah SukarnoIPB University1
Rohani Cinta Badia GintingIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Development of Simple-Sequence Repeats Markers from Durian (Durio zibethinus Murr. cultv. Matahari) Genomic Library</dc:title>
	<dc:creator>Santoso, Panca Jarot</dc:creator>
	<dc:creator>Pancoro, Adi</dc:creator>
	<dc:creator>Suhandono, Sony</dc:creator>
	<dc:creator>Pugeg Aryantha, I Nyoman</dc:creator>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:subject xml:lang="en-US">durian (Durio sp.); genetic markers; informative loci; SSR</dc:subject>
	<dc:description xml:lang="en-US">Simple sequence repeats have been proved as powerful markers and widely used in molecular breeding to reduce cycles and cost efective. The availability of the marker is, however, very limited in durian. This research aimed to develop SSR markers from durian genomic library. Genomic DNA was isolated from durian shoot leaf, whilst SSR motifs were isolated using membrane-based oligonucleotide enrichment hybridization protocol. Annotation made on the library found 527 unique motifs from 354 durian libraries which form 425 loci. The SSR motifs obtained were generally short repeats which reached 89.6 %, whilst longer repeats were found consisted of compound motifs. Eleven loci were selected as representative for further test to prove their informativity. A number of unique allels were successfully amplified from 17 durian genomes. The analysis showed the polymorphic information content (PIC) values ranged from 0.000 to 0.662 with an average of 0.390. The SSR loci also showed their ability to be used for durian diversity analysis as the evident that the loci could be used as genetic markers for assisting further durian breeding program.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
	<dc:contributor xml:lang="en-US">Indonesia Agency of Agricultural Research and Development</dc:contributor>
	<dc:date>2017-08-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/1171</dc:identifier>
	<dc:identifier>10.17503/agrivita.v39i3.1171</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 39, No 3 (2017): OCTOBER; 257-265</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v39i3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/1171/870</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2017 UNIVERSITAS BRAWIJAYA</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>