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Developments of Rice Cell Suspension Culture and A Novel Strategy for Screening New Resistant Lines to Rice Blight Disease Caused by Xanthomonas oryzae pv. oryzae
AGRIVITA, Journal of Agricultural Science
ISSN 2477-8516, 0126-0537
Terbit 2018-09-07
Penerbit Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Bahasa eng
Abstrak
The research aimed to develop a rice cell culture system with high proliferation and screening resistant cell lines of rice to bacterial blight disease caused by Xanthomonas oryza pv. oryzae (Xoo). The culture cells obtained from the callus, cultured on CS-1 medium containing 3 % sucrose and 2 mg L-1 2,4-D for 4 weeks. The results showed that proliferation cell was signifcantly increased 1-fold in 3 weeks of primary culture in CS-1 conditioned medium (fresh/spent medium ratio 1:1) containing 3 % sucrose, 0.5 % glucose, 0.05 % fructose and 2 mg L-1 2,4-D. This medium was used to screen the cell lines through applying culture filtrate of Xoo. The method was to find a novel cell line which could produce high amounts of reactive oxygen species (ROS). Screening results showed 33 % cell lines were strong ROS-producing, two cell lines were selected and cultured for second round screening. The ratio of strong ROS-producing cell lines was increased up to 67 % in the third round screening. The strong ROS-producing cell lines in third round screening can be further cultured for plant regeneration. The rice cell lines with high ROS production may have potential of resistant cell lines against Xoo.
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Sumber metadata: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc)
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<dc:title xml:lang="en-US">Developments of Rice Cell Suspension Culture and A Novel Strategy for Screening New Resistant Lines to Rice Blight Disease Caused by Xanthomonas oryzae pv. oryzae</dc:title>
<dc:creator>Kusuma, Restu Rizkyta</dc:creator>
<dc:creator>Sulistyowati, Liliek</dc:creator>
<dc:creator>Cheng, Chiu-Chsiung</dc:creator>
<dc:creator>Lin, Yi-Hsien</dc:creator>
<dc:subject xml:lang="en-US">disease-plant sciences; bacteriology; plant pathology; phytopathology</dc:subject>
<dc:subject xml:lang="en-US">Bacterial blight; Cell culture; Reactive oxygen species; Resistant cell lines; Screening</dc:subject>
<dc:description xml:lang="en-US">The research aimed to develop a rice cell culture system with high proliferation and screening resistant cell lines of rice to bacterial blight disease caused by Xanthomonas oryza pv. oryzae (Xoo). The culture cells obtained from the callus, cultured on CS-1 medium containing 3 % sucrose and 2 mg L-1 2,4-D for 4 weeks. The results showed that proliferation cell was signifcantly increased 1-fold in 3 weeks of primary culture in CS-1 conditioned medium (fresh/spent medium ratio 1:1) containing 3 % sucrose, 0.5 % glucose, 0.05 % fructose and 2 mg L-1 2,4-D. This medium was used to screen the cell lines through applying culture filtrate of Xoo. The method was to find a novel cell line which could produce high amounts of reactive oxygen species (ROS). Screening results showed 33 % cell lines were strong ROS-producing, two cell lines were selected and cultured for second round screening. The ratio of strong ROS-producing cell lines was increased up to 67 % in the third round screening. The strong ROS-producing cell lines in third round screening can be further cultured for plant regeneration. The rice cell lines with high ROS production may have potential of resistant cell lines against Xoo.</dc:description>
<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
<dc:contributor xml:lang="en-US">National Pingtung University of Science and Technology, Department of Plant Medicine</dc:contributor>
<dc:date>2018-09-07</dc:date>
<dc:type>info:eu-repo/semantics/article</dc:type>
<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
<dc:format>application/pdf</dc:format>
<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/1779</dc:identifier>
<dc:identifier>10.17503/agrivita.v40i3.1779</dc:identifier>
<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 40, No 3 (2018): OCTOBER; 434-441</dc:source>
<dc:source>2477-8516</dc:source>
<dc:source>0126-0537</dc:source>
<dc:source>10.17503/agrivita.v40i3</dc:source>
<dc:language>eng</dc:language>
<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/1779/971</dc:relation>
<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/1779/1179</dc:relation>
<dc:rights xml:lang="en-US">Copyright (c) 2018 UNIVERSITAS BRAWIJAYA</dc:rights>
<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>
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APA 7
Kusuma, R. R., Sulistyowati, L., Cheng, C. C., & Lin, Y. H. (2018). Developments of Rice Cell Suspension Culture and A Novel Strategy for Screening New Resistant Lines to Rice Blight Disease Caused by Xanthomonas oryzae pv. oryzae. AGRIVITA, Journal of Agricultural Science, 40(3), 434–441. https://doi.org/10.17503/agrivita.v40i3.1779
Salin
IEEE
R. R. Kusuma, L. Sulistyowati, C. C. Cheng, and Y. H. Lin, “Developments of Rice Cell Suspension Culture and A Novel Strategy for Screening New Resistant Lines to Rice Blight Disease Caused by Xanthomonas oryzae pv. oryzae,” AGRIVITA, Journal of Agricultural Science, vol. 40, no. 3, pp. 434–441, 2018, doi: 10.17503/agrivita.v40i3.1779.
Salin
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