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Genetic Diversity Evaluation of Moringa oleifera, Lam from East Flores Regency Using Marker Random Amplified Polymorphic DNA (RAPD) and Its Relationship to Chemical Composition and In Vitro Gas Production

AGRIVITA, Journal of Agricultural Science · Vol. 39 No. 2 (2017) · hlm. 219–231

DOI
10.17503/agrivita.v39i2.1027
ISSN
2477-8516, 0126-0537
Terbit
2017-04-05
Penerbit
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

The research objective was to evaluate the genetic diversity of Moringa oleifera, Lam (MO) and its relationship to chemical composition and in vitro gas production (IVGP). Fresh MO leaves were kept frozen in ice gels pack until laboratory analysis. Four methods applied: RAPD marker for measuring DNA concentration and purification; Kjeldhal and HPLC for analysing proximate and amino acid (AA) composition; and IVGP. MO’s four distinct morphology found: green, red, reddish green and aromatic green. RAPD result analysis was 68.8-74.7 %, it means those MO had a close genetic similarity. The morphological differences are also related to leaves chemical composition variation. The highest protein and AAs content were found in aromatic green MO. Total IVGP at 96 hours reached 95.9, 99.3, 111, 115 mL per 500 mg DM in aromatic green, green, reddish green, red MO, respectively and statistically among those was highly significant difference (P<0.01). However, DM and OM digestibility did not differ significantly and estimated ME contents were similar suggesting MO leaves had sufficient fermentable nitrogen amount required to ensure rumen microbes normal activities. Conclusively, those MO has a close genetic relationship but the aromatic green MO more beneficial due its higher content of crude protein and AAs.

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Budi WaluyoWalailak University4
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Irzal AndersonAfiliasi tidak diketahui2
Arifin Noor SugihartoAfiliasi tidak diketahui2
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Melyndra Sonya PramitaAfiliasi tidak diketahui1
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Gita Novita SariAfiliasi tidak diketahui1
Farida FaridaAfiliasi tidak diketahui1
Nunun BarunawatiUniversity of Brawijaya1
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Sumber metadata: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Genetic Diversity Evaluation of Moringa oleifera, Lam from East Flores Regency Using Marker Random Amplified Polymorphic DNA (RAPD) and Its Relationship to Chemical Composition and In Vitro Gas Production</dc:title>
	<dc:creator>Kleden, Markus Miten</dc:creator>
	<dc:creator>Soetanto, Hendrawan</dc:creator>
	<dc:creator>Kusmartono, Kusmartono</dc:creator>
	<dc:creator>Kuswanto, Kuswanto</dc:creator>
	<dc:subject xml:lang="en-US">Animal husbandry department of  Animal nutrition</dc:subject>
	<dc:subject xml:lang="en-US">amino acid content; in vitro digestibility; miracle tree; morphological variation</dc:subject>
	<dc:description xml:lang="en-US">The research objective was to evaluate the genetic diversity of Moringa oleifera, Lam (MO) and its relationship to chemical composition and in vitro gas production (IVGP). Fresh MO leaves were kept frozen in ice gels pack until laboratory analysis. Four methods applied: RAPD marker for measuring DNA concentration and purification; Kjeldhal and HPLC for analysing proximate and amino acid (AA) composition; and IVGP. MO’s four distinct morphology found: green, red, reddish green and aromatic green. RAPD result analysis was 68.8-74.7 %, it means those MO had a close genetic similarity. The morphological differences are also related to leaves chemical composition variation. The highest protein and AAs content were found in aromatic green MO. Total IVGP at 96 hours reached 95.9, 99.3, 111, 115 mL per 500 mg DM in aromatic green, green, reddish green, red MO, respectively and statistically among those was highly significant difference (P&amp;lt;0.01). However, DM and OM digestibility did not differ significantly and estimated ME contents were similar suggesting MO leaves had sufficient fermentable nitrogen amount required to ensure rumen microbes normal activities. Conclusively, those MO has a close genetic relationship but the aromatic green MO more beneficial due its higher content of crude protein and AAs.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
	<dc:contributor xml:lang="en-US">the Directorate General of Higher Education, Ministry of Research Technology and Higher Education, the Republic of Indonesia</dc:contributor>
	<dc:date>2017-04-05</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/1027</dc:identifier>
	<dc:identifier>10.17503/agrivita.v39i2.1027</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 39, No 2 (2017): JUNE; 219-231</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v39i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/1027/860</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2017 markus mitten kleden</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>