Purification and Identification of an Antifungal Protein from an Isolated Fungus with Antagonism to Colletotrichum gloeosporioides MC9

Sandy, Yohana Avelia; Chen, Yo-Chia; Sulistyowati, Liliek

doi:10.17503/agrivita.v44i2.2966

Akses terbuka Jurnal SINTA 1

Purification and Identification of an Antifungal Protein from an Isolated Fungus with Antagonism to Colletotrichum gloeosporioides MC9

AGRIVITA, Journal of Agricultural Science · Vol. 44 No. 2 (2022) · hlm. 332–343

DOI: 10.17503/agrivita.v44i2.2966
ISSN: 2477-8516, 0126-0537
Terbit: 2022-06-02
Penerbit: Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Bahasa: eng
Lisensi: creativecommons.org/licenses/by-nc/4.0

Abstrak

Colletotrichum gloeosporioides is the cause of anthracnose disease on mango. This disease becomes more damaging because it economically affects the harvested fruit during the postharvest season. In this research, eight isolates are isolated from the soil of a mango plantation. One of the isolates shows antifungal activity against C. gloeosporioides MC9. This isolate is identified as Penicillium citrinum isolate S1 based on the phylogenetic analysis of ribosomal rRNA sequence. From the culture of this isolate, extracellular filtrates are collected and evaluated for their antifungal activity. The mycelial growth of C. gloeosporioides is significantly inhibited by the culture supernatant of P. citrinum isolate S1. The culture filtrate is used to purify the antifungal protein using ammonium sulfate and ultrafiltration methods. Results show that the antifungal protein was estimated at around 40 kDa molecular weight when separated on a 10% Sodium dodecyl sulfate-polyacrylamide gel. After nine days of incubation, this antifungal protein’s inhibition effect with a concentration of 0.94 mg/ml remained 63.6% against C. gloeosporioides. The LCMS result showed that the antifungal protein belongs to the L-asparaginase superfamily. Based on this result, the antifungal protein produced by P. citrinum S1 has the potential to control mango anthracnose disease caused by C. gloeosporioides.

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13 penulis · 23 tautan kepenulisan bersama · 10 lintas organisasi

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National Pingtung University of Science and Technology
University of Brawijaya
Universitas Jember
National Research and Innovation Agency
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Rekan penulis	Organisasi	Artikel bersama
Luqman Qurata Aini	University of Brawijaya	2
Wita Firdausi	University of Brawijaya	1
Restu Rizkyta Kusuma	National Pingtung University of Science and Technology	1
Chiu-Chsiung Cheng	National Pingtung University of Science and Technology	1
Yi‐Hsien Lin	National Pingtung University of Science and Technology	1
Bambang Sugiharto	Universitas Jember	1
Nur Basuki	University of Brawijaya	1
Slameto Slameto	Afiliasi tidak diketahui	1
Eriyanto Yusnawan	National Research and Innovation Agency	1
Alfi Inayati	University of Brawijaya	1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.

Metadata asli (oai_dc)

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	<dc:title xml:lang="en-US">Purification and Identification of an Antifungal Protein from an Isolated Fungus with Antagonism to Colletotrichum gloeosporioides MC9</dc:title>
	<dc:creator>Sandy, Yohana Avelia</dc:creator>
	<dc:creator>Chen, Yo-Chia</dc:creator>
	<dc:creator>Sulistyowati, Liliek</dc:creator>
	<dc:subject xml:lang="en-US">Disease plant science</dc:subject>
	<dc:subject xml:lang="en-US">Anthracnose Disease; Antifungal Protein; Colletotrichum gloeosporioides; Pathogenic Fungi; Penicillium citrinum</dc:subject>
	<dc:description xml:lang="en-US">Colletotrichum gloeosporioides is the cause of anthracnose disease on mango. This disease becomes more damaging because it economically affects the harvested fruit during the postharvest season. In this research, eight isolates are isolated from the soil of a mango plantation. One of the isolates shows antifungal activity against C. gloeosporioides MC9. This isolate is identified as Penicillium citrinum isolate S1 based on the phylogenetic analysis of ribosomal rRNA sequence. From the culture of this isolate, extracellular filtrates are collected and evaluated for their antifungal activity. The mycelial growth of C. gloeosporioides is significantly inhibited by the culture supernatant of P. citrinum isolate S1. The culture filtrate is used to purify the antifungal protein using ammonium sulfate and ultrafiltration methods. Results show that the antifungal protein was estimated at around 40 kDa molecular weight when separated on a 10% Sodium dodecyl sulfate-polyacrylamide gel. After nine days of incubation, this antifungal protein’s inhibition effect with a concentration of 0.94 mg/ml remained 63.6% against C. gloeosporioides. The LCMS result showed that the antifungal protein belongs to the L-asparaginase superfamily. Based on this result, the antifungal protein produced by P. citrinum S1 has the potential to control mango anthracnose disease caused by C. gloeosporioides.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
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	<dc:date>2022-06-02</dc:date>
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	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/2966</dc:identifier>
	<dc:identifier>10.17503/agrivita.v44i2.2966</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 44, No 2 (2022); 332-343</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v44i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/2966/1489</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/2966/2187</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/2966/2188</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/2966/2568</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2022 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
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