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Effect of Colchicine Concentrations and Soaking Period on Ploidy of Katokkon Chili (Capsicum chinense Jacq.) at Seedlings Stage

AGRIVITA, Journal of Agricultural Science · Vol. 46 No. 3 (2024) · hlm. 506–517

DOI
10.17503/agrivita.v46i3.3936
ISSN
2477-8516, 0126-0537
Terbit
2024-09-05
Penerbit
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

This research is conducted to study the induction of polyploidization in Katokkon chili using colchicine to study the effect of chromosome doubling or polyploid on certain superior characters during the seedling phase. Some morphological alterations were determined and proven to persist during the seedling phase. The study was conducted in the laboratory and screen house of the Department of Agronomy, Faculty of Agriculture, Universitas Hasanuddin. The polyploidy of the Katokkon chili is induced by soaking the germinated seeds in 0%, 0.05%, 0.1%, and 0.2% colchicine solutions for 6, 12, 24, and 48 hours each. Ploidy levels are analyzed using flow cytometry (Partec®Cy-Flow Space TM). Results show significant differences in colchicine treatment affecting all parameters observed: hypocotyl base of sprouts, plant height, stomata size, and the number of lateral shoots. Flow cytometry analysis histogram confirmed that administration of 0.1% colchicine concentration with 48 hours soaking time and 0.2% colchicine concentration with 24- or 48-hour soaking time can induce tetraploid plants (4n=48) with different coefficients of variance (CV-x%), 5.36%, 4.65%, 6.08%, respectively. Vigorous growth phenotype in leaf size and plant height was more clearly seen in the one-month-old tetraploid Katokkon chili seedlings induced by 0.10% with a soaking time of 48 hours.

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  • Hasanuddin University
  • Sekolah Tinggi Ilmu Kesehatan Tamalatea Makassar
  • Universitas Muhammadiyah Sidenreng Rappang
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Rekan penulisOrganisasiArtikel bersama
Hari IswoyoHasanuddin University18
Katriani MantjaHasanuddin University15
Feranita HaringHasanuddin University15
Tigin DariatiHasanuddin University13
Cri Wahyuni Brahmi YantiHasanuddin University11
Rahmansyah DermawanHasanuddin University9
Nurfaida NurfaidaHasanuddin University8
Syatrianty Andi SyaifulHasanuddin University8
NurfaidaHasanuddin University5
Muhammad Fuad AnshoriHasanuddin University5
Nuniek WidiayaniHasanuddin University5
Muhammad FariedHasanuddin University5
Rahmansyah DermawanHasanuddin University5
Elkawakib Syam’unHasanuddin University5
Novaty Eny DunggaHasanuddin University5
Amirullah DachlanHasanuddin University4
RafiuddinAfiliasi tidak diketahui4
A. R. TrisnawatyUniversitas Muhammadiyah Sidenreng Rappang4
Asmiaty SahurHasanuddin University4
Rizza Nurul ApriliaHasanuddin University3
Muh. Farid BdrHasanuddin University3
Muh. RiadiAfiliasi tidak diketahui3
Rafiuddin RafiuddinHasanuddin University3
Feranita HaringAfiliasi tidak diketahui3
Yunus MusaHasanuddin University3
AMIR YASSIHasanuddin University2
Abdul MollahAfiliasi tidak diketahui2
Hatta JamilHasanuddin University2
. NasaruddinAfiliasi tidak diketahui2
Sakka PatiHasanuddin University2
Muh FaridHasanuddin University2
Ahmad Fauzan AdzimaHasanuddin University2
Amin NurSekolah Tinggi Ilmu Kesehatan Tamalatea Makassar2
Rusnadi PadjungHasanuddin University2

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Sumber metadata: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Effect of Colchicine Concentrations and Soaking Period on Ploidy of Katokkon Chili (Capsicum chinense Jacq.) at Seedlings Stage</dc:title>
	<dc:creator>Sjahril, Rinaldi</dc:creator>
	<dc:creator>Riadi, Muhammad</dc:creator>
	<dc:creator>Ridwan, Ifayanti</dc:creator>
	<dc:creator>Kasim, Nurlina</dc:creator>
	<dc:creator>Tambung, Astina</dc:creator>
	<dc:creator>Novitasari, Novitasari</dc:creator>
	<dc:subject xml:lang="en-US">Agronomy</dc:subject>
	<dc:subject xml:lang="en-US">Colchicine; Flow cytometry analysis; Katokkon chili; Polyploidization; Tetraploid</dc:subject>
	<dc:description xml:lang="en-US">This research is conducted to study the induction of polyploidization in Katokkon chili using colchicine to study the effect of chromosome doubling or polyploid on certain superior characters during the seedling phase. Some morphological alterations were determined and proven to persist during the seedling phase. The study was conducted in the laboratory and screen house of the Department of Agronomy, Faculty of Agriculture, Universitas Hasanuddin. The polyploidy of the Katokkon chili is induced by soaking the germinated seeds in 0%, 0.05%, 0.1%, and 0.2% colchicine solutions for 6, 12, 24, and 48 hours each. Ploidy levels are analyzed using flow cytometry (Partec®Cy-Flow Space TM). Results show significant differences in colchicine treatment affecting all parameters observed: hypocotyl base of sprouts, plant height, stomata size, and the number of lateral shoots. Flow cytometry analysis histogram confirmed that administration of 0.1% colchicine concentration with 48 hours soaking time and 0.2% colchicine concentration with 24- or 48-hour soaking time can induce tetraploid plants (4n=48) with different coefficients of variance (CV-x%), 5.36%, 4.65%, 6.08%, respectively. Vigorous growth phenotype in leaf size and plant height was more clearly seen in the one-month-old tetraploid Katokkon chili seedlings induced by 0.10% with a soaking time of 48 hours.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
	<dc:contributor xml:lang="en-US">The Ministry of Education, Culture, Research and Technology of Indonesia</dc:contributor>
	<dc:date>2024-09-05</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/3936</dc:identifier>
	<dc:identifier>10.17503/agrivita.v46i3.3936</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 46, No 3 (2024); 506-517</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v46i3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/3936/1786</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/3936/2711</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/3936/3256</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/3936/3257</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2024 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
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