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Rapid Micropropagation of Alocasia azlanii using Microshoot Tips as Explants Source

AGRIVITA, Journal of Agricultural Science · Vol. 47 No. 3 (2025) · hlm. 462–471

DOI
10.17503/agrivita.v47i3.4838
ISSN
2477-8516, 0126-0537
Terbit
2025-09-18
Penerbit
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Alocasia azlanii is a unique species that has an ornamental value, native to Southeast Asia. The present investigation aimed to develop reliable in vitro propagation techniques for A. azlanii, suitable for potential mutation and polyploidization programs. The in vitro propagation protocol for these species includes sterilization, shoot proliferation, in vitro rooting induction, and acclimation stages. The results showed that the effective protocol for freeing the explants from contamination was achieved through additional pre-treatment with bactericide and fungicide, which produced the best percentage of clean shoot-tip explants (87%). At the shoot multiplication stage, the shoot-tip explant cultivated in MS medium supplemented with 0.05 mg/l TDZ and 1 mg/l BAP showed the highest number of shoots (31.8) in the second subculture cycle. In rooting stages, single shoots that were planted in MS medium supplemented with 0.5 mg/l activated charcoal and 0.5 mg/l NAA resulted in the best plantlet performances in terms of stem diameter, number of roots, number of leaves, and plantlet height. In the acclimatization stage, planting media composed of husk charcoal, soil, manure, and volcanic fine sand mixture (2:1:1:1 v/v) preserved vigorous plantlet development, with 93% survival.

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  • National Research and Innovation Agency
  • Indonesian Institute of Sciences
  • University of Brawijaya
  • Indonesian Agency for Agricultural Research and Development
  • Mindanao State University
  • National Nuclear Energy Agency of Indonesia
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Rekan penulisOrganisasiArtikel bersama
Lia HapsariIndonesian Institute of Sciences7
Budi MarwotoIndonesian Agency for Agricultural Research and Development6
Hanudin HanudinIndonesian Agency for Agricultural Research and Development5
Indijarto Budi RahardjoIndonesian Agency for Agricultural Research and Development5
Dewi LestariNational Research and Innovation Agency5
Wakiah NuryaniIndonesian Agency for Agricultural Research and Development4
Elga RenjanaNational Research and Innovation Agency4
Linda Wige NingrumNational Research and Innovation Agency4
Janis DamaiyaniIndonesian Institute of Sciences4
Evi Silvia YusufAfiliasi tidak diketahui3
Fauziah FauziahIndonesian Institute of Sciences3
Ita DwimahyaniNational Nuclear Energy Agency of Indonesia3
Lia SanjayaNational Research and Innovation Agency3
Melisnawati H. AngioNational Research and Innovation Agency2
Rony IrawantoNational Research and Innovation Agency2
Kuswanto KuswantoUniversity of Brawijaya2
Deden MudianaAfiliasi tidak diketahui1
TrimantoAfiliasi tidak diketahui1
Sugeng BudihartaIndonesian Institute of Sciences1
TITUT YULISTYARINIIndonesian Institute of Sciences1
Setyawan Agung DanartoIndonesian Institute of Sciences1
Rachmawan Adi LaksonoIndonesian Institute of Sciences1
Nurlaily LaviantiAfiliasi tidak diketahui1
Bekti YunantoAfiliasi tidak diketahui1
Indijarto Budi RaharjoAfiliasi tidak diketahui1
Rina ApriantiNational Research and Innovation Agency1
Lukita DevyNational Research and Innovation Agency1
Eka NurhanggaNational Research and Innovation Agency1
Winda NawfetriasNational Research and Innovation Agency1
Amalia Khairunnisa’University of Brawijaya1
Ardiyan TarunaUniversity of Brawijaya1
Rifani Rusiana DewiUniversity of Brawijaya1

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Sumber metadata: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Rapid Micropropagation of Alocasia azlanii using Microshoot Tips as Explants Source</dc:title>
	<dc:creator>Siregar, Ahmad Syahrian</dc:creator>
	<dc:creator>Mas’udah, Shofiyatul</dc:creator>
	<dc:creator>Firdiana, Elok Rifqi</dc:creator>
	<dc:creator>Widiarsih, Sasanti</dc:creator>
	<dc:creator>Budiarto, Kurniawan</dc:creator>
	<dc:creator>Yusuf, Hadi Mokhamad</dc:creator>
	<dc:creator>Yulianah, Izmi</dc:creator>
	<dc:creator>Masnar, Adley L.</dc:creator>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:subject xml:lang="en-US">Acclimatization media; Alocasia; In vitro; Plant growth regulators; Pre-treatments</dc:subject>
	<dc:description xml:lang="en-US">Alocasia azlanii is a unique species that has an ornamental value, native to Southeast Asia. The present investigation aimed to develop reliable in vitro propagation techniques for A. azlanii, suitable for potential mutation and polyploidization programs. The in vitro propagation protocol for these species includes sterilization, shoot proliferation, in vitro rooting induction, and acclimation stages. The results showed that the effective protocol for freeing the explants from contamination was achieved through additional pre-treatment with bactericide and fungicide, which produced the best percentage of clean shoot-tip explants (87%). At the shoot multiplication stage, the shoot-tip explant cultivated in MS medium supplemented with 0.05 mg/l TDZ and 1 mg/l BAP showed the highest number of shoots (31.8) in the second subculture cycle. In rooting stages, single shoots that were planted in MS medium supplemented with 0.5 mg/l activated charcoal and 0.5 mg/l NAA resulted in the best plantlet performances in terms of stem diameter, number of roots, number of leaves, and plantlet height. In the acclimatization stage, planting media composed of husk charcoal, soil, manure, and volcanic fine sand mixture (2:1:1:1 v/v) preserved vigorous plantlet development, with 93% survival.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2025-09-18</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/4838</dc:identifier>
	<dc:identifier>10.17503/agrivita.v47i3.4838</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 47, No 3 (2025); 462-471</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v47i3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/4838/1892</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/4838/3417</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/4838/3418</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2025 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
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