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Cytological Distinctions Between Timun Suri and Cucumber Discovered by Fluorescence In Situ Hybridization (FISH) Using 45S Ribosomal DNA Gene

AGRIVITA, Journal of Agricultural Science · Vol. 42 No. 3 (2020) · hlm. 584–592

DOI
10.17503/agrivita.v42i3.2142
ISSN
2477-8516, 0126-0537
Terbit
2020-10-19
Penerbit
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

The genus Cucumis including timun suri, melon and cucumber, is an important horticultural crop of flowering plants. The dispute of timun suri terminology is leading to false-positive results in the nomenclature of timun suri in Indonesia. Although molecular research on these species has been widely conducted, detailed information and precise evidence based on the molecular cytogenetic approach are poorly investigated. The FISH technique was applied to reveal the cytological distinctions among these species. Here, this research conducted physical mapping of 45S ribosomal DNA (rDNA) on six accessions of Cucumis. The chromosome number of cucumber is 14 chromosomes, while timun suri and melon are 24 chromosomes. The number of 45S rDNA loci in cucumber, melon and timun suri was highly conserved. All cucumber accessions in this study had nine to ten 45S rDNA loci with strong and weak signal intensities located at proximal regions of the short arms. In melon and timun suri the signals of two pairs of 45S rDNA were located at the terminal and interstitial regions of the short arms. These were clear shreds of evidence to confirm that timun suri was cytogenetically closed to melon instead of cucumber. It confirmed that timun suri cannot be classified as a cucumber

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  • Universitas Gadjah Mada
  • Nagoya University
  • UIN Raden Mas Said Surakarta
  • National Research and Innovation Agency
  • Chiba University
  • Indonesian Coffee and Cocoa Research Institute
  • University of Malaya
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Rekan penulisOrganisasiArtikel bersama
Endang SemiartiUniversitas Gadjah Mada11
Chee How TeoUniversity of Malaya6
Sukarti MoeljopawiroUniversitas Gadjah Mada5
Dyah Weny RespatieUniversitas Gadjah Mada4
Ari IndriantoUniversitas Gadjah Mada3
Nintya SetiariDiponegoro University2
Muhammad Dylan LawrieUniversitas Gadjah Mada2
Exsyupransia MursyantiUniversitas Gadjah Mada2
Irna AuliauzzakiaUniversitas Gadjah Mada2
Binti Nur AisahUniversitas Gadjah Mada2
Aprilia Tiara IkhwaniUniversitas Gadjah Mada2
Erlina AmbarwatiUniversitas Gadjah Mada2
Listy AnggraeniNational Research and Innovation Agency2
Chandra SetyawanUniversitas Gadjah Mada2
Aniswatul KhamidahNational Research and Innovation Agency2
Shinji KikuchiChiba University2
Takato KobaChiba University2
Yeni FatmawatiUniversitas Gadjah Mada2
Tsalatsatun Nur HidayahUIN Raden Mas Said Surakarta1
Adillah Khairun Nisa’UIN Raden Mas Said Surakarta1
Michael Hagana BangunAfiliasi tidak diketahui1
Asadi AsadiAfiliasi tidak diketahui1
Sahiral YakubAfiliasi tidak diketahui1
Triono Bagus SaputroInstitut Teknologi Sepuluh Nopember1
Windi MoseUniversitas Pattimura1
Zulfa LayinaUniversitas Gadjah Mada1
Della Rosiana NingtiasUniversitas Gadjah Mada1
Falah Nur AlifiantoUniversitas Gadjah Mada1
Ixora Sartika MercurianiYogyakarta State University1
Seonghoe JangAgricultural Biotechnology Research Center, Academia Sinica1
Jaka WidadaUniversitas Gadjah Mada1
Sri NopitasariUniversitas Gadjah Mada1
Yuli SetiawatiIndonesian Oil Palm Research Institute1
Yasushi YoshiokaNagoya University1
Shogo MatsumotoNagoya University1
Kana NinomiyaNagoya University1
Yuuki AsanoNagoya University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Cytological Distinctions Between Timun Suri and Cucumber Discovered by Fluorescence In Situ Hybridization (FISH) Using 45S Ribosomal DNA Gene</dc:title>
	<dc:creator>Setiawan, Agus Budi</dc:creator>
	<dc:creator>Purwantoro, Aziz</dc:creator>
	<dc:creator>Wibowo, Ari</dc:creator>
	<dc:subject xml:lang="en-US">Plant breeding; cytogenetic; horticulture</dc:subject>
	<dc:subject xml:lang="en-US">45S rDNA; Cucumber; Cucumis melo var. momordica; FISH; Timun suri</dc:subject>
	<dc:description xml:lang="en-US">The genus Cucumis including timun suri, melon and cucumber, is an important horticultural crop of flowering plants. The dispute of timun suri terminology is leading to false-positive results in the nomenclature of timun suri in Indonesia. Although molecular research on these species has been widely conducted, detailed information and precise evidence based on the molecular cytogenetic approach are poorly investigated. The FISH technique was applied to reveal the cytological distinctions among these species. Here, this research conducted physical mapping of 45S ribosomal DNA (rDNA) on six accessions of Cucumis. The chromosome number of cucumber is 14 chromosomes, while timun suri and melon are 24 chromosomes. The number of 45S rDNA loci in cucumber, melon and timun suri was highly conserved. All cucumber accessions in this study had nine to ten 45S rDNA loci with strong and weak signal intensities located at proximal regions of the short arms. In melon and timun suri the signals of two pairs of 45S rDNA were located at the terminal and interstitial regions of the short arms. These were clear shreds of evidence to confirm that timun suri was cytogenetically closed to melon instead of cucumber. It confirmed that timun suri cannot be classified as a cucumber</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2020-10-19</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/2142</dc:identifier>
	<dc:identifier>10.17503/agrivita.v42i3.2142</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 42, No 3 (2020); 584-592</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v42i3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/2142/1316</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/2142/1288</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2020 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>