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Effect of Gamma Rays Irradiation to Cipedak Avocado Genetic Diversity

AGRIVITA, Journal of Agricultural Science · Vol. 45 No. 2 (2023) · hlm. 231–249

DOI
10.17503/agrivita.v45i2.4065
ISSN
2477-8516, 0126-0537
Terbit
2023-05-16
Penerbit
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Avocado cv. Cipedak is a superior cultivar that match the preferences of Indonesian consumers. However, it has several weaknesses, such as low edible portion, thin skin, and short fruit shelf life. One effort to improve the character weakness is by mutation breeding using gamma ray irradiation. Induction of mutation by gamma ray irradiation at a dose of 10 Gy was conducted on Cipedak avocado shoots in October 2021 which produced 13 first generation avocado mutants (M1). Observation on leaves morphological characters and DNA analysis were needed to find out the changes level of genetical diversity. The research was conducted in Cukurgondang Research Station and Molecular Laboratory of Indonesian Tropical Fruits Research Institute from January to March 2022. The plant material used involved 13 M1 progenies and cv. Cipedak. The material used were 20 weeks old grafted plants. The observed variables were macro and micromorphological characters as well as DNA-RAPD analysis. The results showed that there were phenotypic and genotypic changes in 13 M1 avocados seedlings. The coefficients of differences on macro and micromorphological characters were up to 33% and 28%, respectively and the coefficient of difference for DNA-RAPD analysis was 74%.

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  • National Research and Innovation Agency
  • University of Brawijaya
  • Universitas Gadjah Mada
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Rekan penulisOrganisasiArtikel bersama
Handoko HandokoAfiliasi tidak diketahui2
Riry PrihatiniNational Research and Innovation Agency1
Leni MarlinaNational Research and Innovation Agency1
Bambang HariyantoNational Research and Innovation Agency1
Eliza MayuraNational Research and Innovation Agency1
Febria Cahya IndrianiUniversity of Brawijaya1
Nur BasukiUniversity of Brawijaya1
M. JusufAfiliasi tidak diketahui1
Celia dela Rosa MedinaAfiliasi tidak diketahui1
Izmi YulianahAfiliasi tidak diketahui1
Budi WaluyoAfiliasi tidak diketahui1
Niken KendariniUniversity of Brawijaya1
M. JusufAfiliasi tidak diketahui1
Suputa SuputaUniversitas Gadjah Mada1
Riya Fatma SariUniversitas Gadjah Mada1
Nugroho Susetya PutraUniversitas Gadjah Mada1
Dirham DirhamUniversitas Gadjah Mada1
Deni PranowoUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Effect of Gamma Rays Irradiation to Cipedak Avocado Genetic Diversity</dc:title>
	<dc:creator>Ihsan, Farihul</dc:creator>
	<dc:creator>Ashari, Sumeru</dc:creator>
	<dc:creator>Soegianto, Andy</dc:creator>
	<dc:creator>Sukartini, Sukartini</dc:creator>
	<dc:creator>Affandi, Affandi</dc:creator>
	<dc:subject xml:lang="en-US">plant breeding</dc:subject>
	<dc:subject xml:lang="en-US">Cipedak avocado; DNA-RAPD; gamma ray irradiation; leaf morphology</dc:subject>
	<dc:description xml:lang="en-US">Avocado cv. Cipedak is a superior cultivar that match the preferences of Indonesian consumers. However, it has several weaknesses, such as low edible portion, thin skin, and short fruit shelf life. One effort to improve the character weakness is by mutation breeding using gamma ray irradiation. Induction of mutation by gamma ray irradiation at a dose of 10 Gy was conducted on Cipedak avocado shoots in October 2021 which produced 13 first generation avocado mutants (M1). Observation on leaves morphological characters and DNA analysis were needed to find out the changes level of genetical diversity. The research was conducted in Cukurgondang Research Station and Molecular Laboratory of Indonesian Tropical Fruits Research Institute from January to March 2022. The plant material used involved 13 M1 progenies and cv. Cipedak. The material used were 20 weeks old grafted plants. The observed variables were macro and micromorphological characters as well as DNA-RAPD analysis. The results showed that there were phenotypic and genotypic changes in 13 M1 avocados seedlings. The coefficients of differences on macro and micromorphological characters were up to 33% and 28%, respectively and the coefficient of difference for DNA-RAPD analysis was 74%.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
	<dc:contributor xml:lang="en-US">Department of Agronomy, Faculty of Agriculture, Brawijaya University</dc:contributor>
	<dc:date>2023-05-16</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/4065</dc:identifier>
	<dc:identifier>10.17503/agrivita.v45i2.4065</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 45, No 2 (2023); 231-249</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v45i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/4065/1551</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/4065/2770</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/4065/2856</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2023 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>