IN VITRO SHOOT REGENERATION OF INDONESIAN BANANAS (Musa spp.) cv. AMBON KUNING AND RAJA BULU, PLANTLET ACCLIMATIZATIONAND FIELD PERFORMANCE

Yusnita, Yusnita; Danial, Ekawati; Hapsoro, Dwi

doi:10.17503/agrivita.v37i1.438

Akses terbuka Jurnal SINTA 1

IN VITRO SHOOT REGENERATION OF INDONESIAN BANANAS (Musa spp.) cv. AMBON KUNING AND RAJA BULU, PLANTLET ACCLIMATIZATIONAND FIELD PERFORMANCE

AGRIVITA, Journal of Agricultural Science · Vol. 37 No. 1 (2015) · hlm. 51–58

DOI: 10.17503/agrivita.v37i1.438
ISSN: 2477-8516, 0126-0537
Terbit: 2015-02-05
Penerbit: Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Bahasa: eng
Lisensi: creativecommons.org/licenses/by-nc/4.0/

Abstrak

The aim of this study was to observe responses of two banana cultivars ‘Ambon’ and ‘Raja Bulu’ on different BA concentrations and effects of different media and fertilizer on survival and growth of plantlets. Sterilized explants were cultured on initiation medium (MS with 1mg L-1 BA) for 4 weeks, then subjected to media MS with 2.5, 5.0, or 7.5 mg L-1 BA. Numbers of shoot buds, shoots and propagules were recorded after 4 consecutive passages with 4 weeks intervals. Rooted plantlets were acclimatized in three different media, then treated with or without NPK (32:10:10) fertilizer solution once a week. After 2 months, the survival and growth of plantlets were recorded. Cultures of banana ‘Ambon Kuning’ showed higher regenerative capacity compared to ‘Raja Bulu’, producing higher numbers of shoot buds, shoots and propagules. The best medium for propagule proliferation of both banana cultivars was MS+5 mg L-1 BA, producing 40.7 propagules for ‘Ambon Kuning’, and 12.3 propagules for ‘Raja Bulu’ per explant. In all acclimatization media tested, 100% of plantlet survival was achieved. The best plantlet growth was found in sand: compost (1:1,v/v) with application of NPK solutions. The in vitro-derived plants were planted in the field and produced fruits of high quality.

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Universitas Halu Oleo
University of Timor
State University of Jakarta
University of Brawijaya
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Rekan penulis	Organisasi	Artikel bersama
La Ode Herman Halika	Afiliasi tidak diketahui	1
Novi Novi	Afiliasi tidak diketahui	1
Joko Joko	Afiliasi tidak diketahui	1
Joko Joko	Universitas Halu Oleo	1
La Iba	Universitas Halu Oleo	1
Fitriani Fitriani	University of Timor	1
Adrian Tawai	Universitas Halu Oleo	1
Sudarsono Sudarsono	University of Brawijaya	1
Widodo Widodo	State University of Jakarta	1

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Sumber metadata: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.

Metadata asli (oai_dc)

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	<dc:title xml:lang="en-US">IN VITRO SHOOT REGENERATION OF INDONESIAN BANANAS (Musa spp.) cv. AMBON KUNING AND RAJA BULU, PLANTLET ACCLIMATIZATIONAND FIELD PERFORMANCE</dc:title>
	<dc:creator>Yusnita, Yusnita</dc:creator>
	<dc:creator>Danial, Ekawati</dc:creator>
	<dc:creator>Hapsoro, Dwi</dc:creator>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:subject xml:lang="en-US">acclimatization; benzyladenine; fertilizer; genotypes;  media mixtures</dc:subject>
	<dc:description xml:lang="en-US">The aim of this study was to observe responses of two banana cultivars ‘Ambon’ and ‘Raja Bulu’ on different BA concentrations and effects of different media and fertilizer on survival and growth of plantlets.  Sterilized explants were cultured on initiation medium (MS with 1mg L-1 BA) for 4 weeks, then subjected to media MS with 2.5, 5.0, or 7.5 mg L-1 BA. Numbers of shoot buds, shoots and propagules were recorded after 4 consecutive passages with 4 weeks intervals.  Rooted plantlets were acclimatized in three different media, then treated with or without NPK (32:10:10) fertilizer solution once a week. After 2 months, the survival and growth of plantlets were recorded. Cultures of banana ‘Ambon Kuning’ showed higher regenerative capacity compared to ‘Raja Bulu’, producing higher numbers of shoot buds, shoots and propagules. The best medium for propagule proliferation of both banana cultivars was MS+5 mg L-1 BA, producing 40.7 propagules for ‘Ambon Kuning’, and 12.3 propagules for ‘Raja Bulu’ per explant. In all acclimatization media tested, 100% of plantlet survival was achieved. The best plantlet growth was found in sand: compost (1:1,v/v) with application of NPK solutions. The in vitro-derived plants were planted in the field and produced fruits of high quality.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2015-02-05</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/438</dc:identifier>
	<dc:identifier>10.17503/agrivita.v37i1.438</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 37, No 1 (2015): FEBRUARY; 51-58</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v37i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/438/472</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 AGRIVITA Journal of Agricultural Science</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0/</dc:rights>
</oai_dc:dc>