Melola Discovery

← Melola Discovery

Akses terbuka Jurnal SINTA 1

Response of Rice Somatic Embryogenesis to Exogenous Melatonin About Its Role in Scavenging Reactive Oxygen Species

AGRIVITA, Journal of Agricultural Science · Vol. 46 No. 1 (2024) · hlm. 48–64

DOI
10.17503/agrivita.v46i1.4060
ISSN
2477-8516, 0126-0537
Terbit
2024-01-10
Penerbit
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

The success rate of explant morphogenesis in plant breeding using tissue culture techniques is frequently plagued by browning due to the oxidation of phenolic compounds. The cumulated amount of reactive oxygen species (ROS) drives the oxidation of phenolic compounds. Melatonin is reported to take a part in modulating the regulation of antioxidant gene expression, reducing the accumulation of reactive oxygen species, and enhancing the efficacy of tissue culture. This study aims to determine the optimal melatonin concentration on the efficiency of plantlet regeneration and expression of the antioxidant resistance gene in rice callus. This study utilizes rice TN1, Gogo Niti II, Ketan Hitam, and Cigeulis cultivars. Melatonin at 0, 10, and 15 µM concentrations is supplemented in plantlet regeneration media. Rice antioxidant-related genes, Mn-SOD, Cu/ZnSOD, Cytosolic APX, CAT, GPOD, OsAPX, and OsCATA, expressed after melatonin supplementation. Melatonin concentration at 10 µM generates the highest expression of all tested genes in TN1 compared to other varieties. The cumulated amount of hydrogen peroxide (H2O2) shows that Melatonin has the potential to increase the proportion of plant regeneration in Cigeulis (90.48%) and Ketan Hitam (91.67%) varieties with a concentration of 10 µM and in TN1 (94.44%) and Gogo Niti II (80%) at a concentration of 15 µM.

Kata kunci

Jaringan kolaborasi para penulis

36 penulis · 126 tautan kepenulisan bersama · 1 lintas organisasi

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • Universitas Jember
  • University of Sarjanawiyata Tamansiswa
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Didik Pudji RestantoAfiliasi tidak diketahui3
Agung Nugroho PuspitoAfiliasi tidak diketahui3
Kyung-Min KimAfiliasi tidak diketahui3
Tri HandoyoAfiliasi tidak diketahui2
Yusuf Dary AhnafAfiliasi tidak diketahui2
IryonoAfiliasi tidak diketahui2
Sri HartatikAfiliasi tidak diketahui2
Mitha Aprilia MufadilahAfiliasi tidak diketahui2
Nurhaliza ThamrinAfiliasi tidak diketahui2
Bambang SugihartoAfiliasi tidak diketahui2
Popy Hartatie HardjoAfiliasi tidak diketahui1
Mohammad Candra PrayogaAfiliasi tidak diketahui1
Sigit SoeparjonoAfiliasi tidak diketahui1
Numalasari NumalasariAfiliasi tidak diketahui1
Falin Fatimatus ShahroAfiliasi tidak diketahui1
Fakhriyah Nur KhalilahAfiliasi tidak diketahui1
Hayati Aulia MaharaniAfiliasi tidak diketahui1
Mohammad Nur KhozinAfiliasi tidak diketahui1
Dewi Kusuma WardaniUniversity of Sarjanawiyata Tamansiswa1
Sholeh AviviUniversitas Jember1
Eviyanti ChoirunnisaAfiliasi tidak diketahui1
Noor RozzitaAfiliasi tidak diketahui1
Mohammad Nur KhozinAfiliasi tidak diketahui1
Kacung HariyonoUniversitas Jember1
Ihsan HabibiAfiliasi tidak diketahui1
Angger Aisyah Hadiahning GustiAfiliasi tidak diketahui1
Rosalina Diva AurelaAfiliasi tidak diketahui1
Rikno HarmokoAfiliasi tidak diketahui1
Siti Nurul AfidahAfiliasi tidak diketahui1
Inyana Dwi AgustienAfiliasi tidak diketahui1
Wahyu Indra Duwi FanataAfiliasi tidak diketahui1
Syafira Fatihatul HusnaAfiliasi tidak diketahui1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Response of Rice Somatic Embryogenesis to Exogenous Melatonin About Its Role in Scavenging Reactive Oxygen Species</dc:title>
	<dc:creator>Ubaidillah, Mohammad</dc:creator>
	<dc:creator>Al Ayyubi, Nabila Nur Aisyah</dc:creator>
	<dc:creator>Khofifa, Rendryana Aulia Nur</dc:creator>
	<dc:creator>Dewanti, Parawita</dc:creator>
	<dc:subject xml:lang="en-US">agronomy</dc:subject>
	<dc:subject xml:lang="en-US">Antioxidant; Gene Expression; Melatonin; Morphogenesis; Tissue Culture</dc:subject>
	<dc:description xml:lang="en-US">The success rate of explant morphogenesis in plant breeding using tissue culture techniques is frequently plagued by browning due to the oxidation of phenolic compounds. The cumulated amount of reactive oxygen species (ROS) drives the oxidation of phenolic compounds. Melatonin is reported to take a part in modulating the regulation of antioxidant gene expression, reducing the accumulation of reactive oxygen species, and enhancing the efficacy of tissue culture. This study aims to determine the optimal melatonin concentration on the efficiency of plantlet regeneration and expression of the antioxidant resistance gene in rice callus. This study utilizes rice TN1, Gogo Niti II, Ketan Hitam, and Cigeulis cultivars. Melatonin at 0, 10, and 15 µM concentrations is supplemented in plantlet regeneration media. Rice antioxidant-related genes, Mn-SOD, Cu/ZnSOD, Cytosolic APX, CAT, GPOD, OsAPX, and OsCATA, expressed after melatonin supplementation. Melatonin concentration at 10 µM generates the highest expression of all tested genes in TN1 compared to other varieties. The cumulated amount of hydrogen peroxide (H2O2) shows that Melatonin has the potential to increase the proportion of plant regeneration in Cigeulis (90.48%) and Ketan Hitam (91.67%) varieties with a concentration of 10 µM and in TN1 (94.44%) and Gogo Niti II (80%) at a concentration of 15 µM.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
	<dc:contributor xml:lang="en-US">University of Jember</dc:contributor>
	<dc:date>2024-01-10</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/4060</dc:identifier>
	<dc:identifier>10.17503/agrivita.v46i1.4060</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 46, No 1 (2024); 48-64</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v46i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/4060/1665</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/4060/3083</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/4060/3084</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/4060/3085</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2024 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>