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OBTAINING Artemisia cina POLYPLOIDY THROUGH PLANT GROWTH REGULATOR TREATMENT IN SHOOT CULTURE

AGRIVITA, Journal of Agricultural Science · Vol. 37 No. 2 (2015) · hlm. 178–184

DOI
10.17503/agrivita.v37i2.490
ISSN
2477-8516, 0126-0537
Terbit
2015-05-27
Penerbit
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0/

Abstrak

Artemisia cina is a medicinal plant species which produces bioactive compound potential to anti-tumor, antifungal and antibacterial medicines. This study was aimed to obtain A. cina polyploid plants through a treatment of growth regulators in shoot culture. The shoot were treated in 1; 15; 2; and 3 mg L-1 of 2,4-dichloro phenoxy acetic acid (2,4-D) combined with 1; 1.5; 2; 3 mg L-1 of Benzyladenine (BA) for 28 days. Chromosome analysis showed that the highest polyploidy percentage, 23%, was reached in the treatment of 2 mg L-1 of 2,4-D combined with 1 of BA mg L-1. The polyploidy level varied, comprising 2n=3x, 2n=4x, 2n=5x, 2n=6x, with the highest polyploidy level percentage, 28.57%, and it was attained in the tetraploid (2n=4x). Polyploid plants had larger leaves area, larger stomatal size, and higher chlorophyll content than diploid plants. However stomatal density of polyploidy plants was lower than that of in diploid plants.

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Sumber metadata: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">OBTAINING Artemisia cina POLYPLOIDY THROUGH PLANT GROWTH  REGULATOR TREATMENT IN SHOOT CULTURE</dc:title>
	<dc:creator>Herawati, Maria Marina</dc:creator>
	<dc:creator>Pudjihartati, Endang</dc:creator>
	<dc:creator>Pramono, Suwijiyo</dc:creator>
	<dc:creator>Sulistyaningsih, Endang</dc:creator>
	<dc:creator>Purwantoro, Aziz</dc:creator>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:subject xml:lang="en-US">2,4-D; Artemisia cina; BA; polyploidy</dc:subject>
	<dc:description xml:lang="en-US">Artemisia cina is a medicinal plant species which produces bioactive compound potential to anti-tumor, antifungal and antibacterial medicines. This study was aimed to obtain A. cina polyploid plants through a treatment of growth regulators in shoot culture. The shoot were treated in 1; 15; 2; and 3 mg L-1 of 2,4-dichloro phenoxy acetic acid (2,4-D) combined with 1; 1.5; 2; 3 mg L-1 of Benzyladenine (BA) for 28 days. Chromosome analysis showed that the highest polyploidy percentage, 23%, was reached in the treatment of 2 mg L-1 of 2,4-D combined with 1 of BA mg L-1. The polyploidy level varied, comprising 2n=3x, 2n=4x, 2n=5x, 2n=6x, with the highest polyploidy level percentage, 28.57%, and it was attained in the tetraploid (2n=4x). Polyploid plants had larger leaves area, larger stomatal size, and higher chlorophyll content than diploid plants. However stomatal density of polyploidy plants was lower than that of in diploid plants.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
	<dc:contributor xml:lang="en-US">Directorate General of Higher Education</dc:contributor>
	<dc:date>2015-05-27</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/490</dc:identifier>
	<dc:identifier>10.17503/agrivita.v37i2.490</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 37, No 2 (2015): JUNE; 178-184</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v37i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/490/639</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 AGRIVITA Journal of Agricultural Science</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0/</dc:rights>
</oai_dc:dc>