Melola Discovery

← Melola Discovery

Akses terbuka Jurnal SINTA 1

In Vitro Assessment of Endophytic Bacteria Originating from Cycas rumphii and Dioon spinulosum as Stress-Tolerant Plant Growth-Promoting Bacteria

AGRIVITA, Journal of Agricultural Science · Vol. 48 No. 1 (2026) · hlm. 153–164

DOI
10.17503/agrivita.v48i1.5072
ISSN
2477-8516, 0126-0537
Terbit
2026-01-14
Penerbit
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

The ability of cycads to grow in harsh environments is thought to be associated with endophytic bacteria in their roots. This study aimed to characterize endophytic bacteria from Cycas rumphii and Dioon spinulosum as stress-tolerant plant growth-promoting bacteria (PGPB). Fine roots of C. rumphii and D. spinulosum were isolated using serial dilution plating and characterized through colony morphological observation, hypersensitivity, antagonistic activity, PGP activity, and abiotic stress tolerance assays. Fourteen isolates were obtained (eight from C. rumphii and six from D. spinulosum), with higher abundance and diversity in D. spinulosum (2.51 × 10¹⁰ CFU/g; H′ = 2.016) than C. rumphii (4.64 × 10⁹ CFU/g; H′ = 1.752). All isolates were non-pathogenic; 92% fixed nitrogen, 92% solubilized phosphate, and one (DS1A) produced IAA (0.200 ppm). Four isolates showed antagonism against Xanthomonas campestris (0.40–0.67 cm inhibition) and tolerance to drought, salinity (5–10%), and moderate heat, with growth at pH 5–6. Molecular identification by 16S rRNA sequencing revealed that CR1A, CR1B, and DS1B belong to Stenotrophomonas maltophilia, whereas DS1A belongs to Bacillus sp. These findings highlight cycads as a source of endophytic PGPB candidates for supporting plant health, particularly when grown in the environmental stress condition areas.

Kata kunci

Jaringan kolaborasi para penulis

40 penulis · 113 tautan kepenulisan bersama · 40 lintas organisasi · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • University of Brawijaya
  • Bandung Institute of Technology
  • The University of Osaka
  • Universitas Islam Negeri Maulana Malik Ibrahim
  • IPB University
  • National Research and Innovation Agency
  • Indonesian Agency for Agricultural Instruments Standardization
  • Organisasi lain
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Abdul Latief AbadiUniversity of Brawijaya4
Hagus TarnoUniversity of Brawijaya3
Eiichiro FukusakiThe University of Osaka3
Liliek SulistyowatiUniversity of Brawijaya3
Ito FernandoUniversity of Brawijaya3
Wita FirdausiUniversity of Brawijaya2
Adi SetiawanUniversity of Brawijaya2
Nurul AiniUniversity of Brawijaya2
Wiwin Sumiya Dwi YamikaUniversity of Brawijaya2
Muhammad Akhid Syib’liUniversity of Brawijaya2
Pingkan AditiawatiBandung Institute of Technology2
Abdullah TaufiqIndonesian Agency for Agricultural Instruments Standardization2
Alfi InayatiUniversity of Brawijaya2
Erfan Dani SeptiaUniversitas Muhammadiyah Malang1
Ardiyan TarunaUniversity of Brawijaya1
Rifani Rusiana DewiUniversity of Brawijaya1
Syarifah Maulidya RahmahUniversity of Brawijaya1
Ruri Siti ResmisariUniversitas Islam Negeri Maulana Malik Ibrahim1
Didik WahyudiUniversitas Islam Negeri Maulana Malik Ibrahim1
Suyono SuyonoUniversitas Islam Negeri Maulana Malik Ibrahim1
Bagas PratesoUniversitas Islam Negeri Maulana Malik Ibrahim1
Annisa AmaliaBandung Institute of Technology1
Fenny Martha DwivanyBandung Institute of Technology1
Kana YamamotoThe University of Osaka1
Made AstawanIPB University1
Tutik WresdiyatiIPB University1
Inas Suci RahmawatiIPB University1
Ananda Putri CahyaniIPB University1
Dea Indriani AstutiBandung Institute of Technology1
Arisa SatoThe University of Osaka1
Gede SuantikaBandung Institute of Technology1
Magdalena Lenny SitumorangBandung Institute of Technology1
Felicia Irene SaputraBandung Institute of Technology1
Ulya AlviredietaBandung Institute of Technology1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">In Vitro Assessment of Endophytic Bacteria Originating from Cycas rumphii and Dioon spinulosum as Stress-Tolerant Plant Growth-Promoting Bacteria</dc:title>
	<dc:creator>Al Husni, Muhammad Fariq</dc:creator>
	<dc:creator>Trianti, Irisa</dc:creator>
	<dc:creator>Yusnawan, Eriyanto</dc:creator>
	<dc:creator>Pamungkas, Bayu Aji</dc:creator>
	<dc:creator>Putri, Sastia Prama</dc:creator>
	<dc:creator>Aini, Luqman Qurata</dc:creator>
	<dc:subject xml:lang="en-US">pest and disease</dc:subject>
	<dc:subject xml:lang="en-US">Cycas rumphii; Dioon spinulosum; Endophytic bacteria; Plant growth-promoting bacteria; Stress tolerance</dc:subject>
	<dc:description xml:lang="en-US">The ability of cycads to grow in harsh environments is thought to be associated with endophytic bacteria in their roots. This study aimed to characterize endophytic bacteria from Cycas rumphii and Dioon spinulosum as stress-tolerant plant growth-promoting bacteria (PGPB). Fine roots of C. rumphii and D. spinulosum were isolated using serial dilution plating and characterized through colony morphological observation, hypersensitivity, antagonistic activity, PGP activity, and abiotic stress tolerance assays. Fourteen isolates were obtained (eight from C. rumphii and six from D. spinulosum), with higher abundance and diversity in D. spinulosum (2.51 × 10¹⁰ CFU/g; H′ = 2.016) than C. rumphii (4.64 × 10⁹ CFU/g; H′ = 1.752). All isolates were non-pathogenic; 92% fixed nitrogen, 92% solubilized phosphate, and one (DS1A) produced IAA (0.200 ppm). Four isolates showed antagonism against Xanthomonas campestris (0.40–0.67 cm inhibition) and tolerance to drought, salinity (5–10%), and moderate heat, with growth at pH 5–6. Molecular identification by 16S rRNA sequencing revealed that CR1A, CR1B, and DS1B belong to Stenotrophomonas maltophilia, whereas DS1A belongs to Bacillus sp. These findings highlight cycads as a source of endophytic PGPB candidates for supporting plant health, particularly when grown in the environmental stress condition areas.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
	<dc:contributor xml:lang="en-US">Irisa Trianti, Plant Pest and Disease Department, Faculty of Agriculture, Universitas Brawijaya</dc:contributor>
	<dc:contributor xml:lang="en-US">Eriyanto Yusnawan, Research Centre for Food Crops, Research Organization for Agriculture and Food, National Research and Innovation Agency (BRIN)</dc:contributor>
	<dc:contributor xml:lang="en-US">Bayu Aji Pa</dc:contributor>
	<dc:date>2026-01-14</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/5072</dc:identifier>
	<dc:identifier>10.17503/agrivita.v48i1.5072</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 48, No 1 (2026); 153-164</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v48i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/5072/1945</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2026 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>