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DISCRIMINATION OF TWO SPECIES OF ORANGUTANS (Pongo sp.): A RAPID PROTOCOL FOR REHABILITATION CENTRES AND ZOOS

BIOTROPIA · Vol. 16 No. 2 (2024)

DOI
10.11598/btb.2009.16.2.52
ISSN
1907-770X, 0215-6334
Terbit
2024-01-11
Penerbit
SEAMEO BIOTROP
Bahasa
en
Lisensi
creativecommons.org/licenses/by-nc-nd/4.0

Abstrak

    Currently orangutans exist in increasingly fragmented and isolated populations. While Sumatran orangutan is primarily found in northern Sumatra, the Bornean is distributed in Central, West, and East Kalimantan, Sarawak and Sabah. The determination of intra- and inter-species variation between Bornean and Sumatran orangutans has been stated to be essential for both the management of orangutan reintroduction projects and the planning of conservation strategies to preserve the remaining wild populations. This study aimed to identify two species of Orangutans (Pongo sp.) by means of RFLP (Restriction Fragment Length Polymorphisms) analyses of mitochondrial DNA (mtDNA). An approximately 540 bp single fragment of the ND5 gene near the 5’-region was PCR amplified for all samples tested. Digestion pattern for both AluI and MseI were different between two groups of ND5 fragments in this study. Present result showed a rapid protocol to identify these two species by means of RFLP (Restriction Fragment Length Polymorphism) analyses of mtDNA (mitochondrial DNA). This technique can be applied easily to rehabilitation centres and zoos to resolve species discrimination problem. Keywords: Orangutans, Pongo sp., Sumatra, Borneo, discrimination

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  • IPB University
  • Liverpool John Moores University
  • National Research and Innovation Agency
  • University of Zurich
  • German Primate Center
  • Czech University of Life Sciences Prague
  • Institut Teknologi Kalimantan
  • Organisasi lain
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Rekan penulisOrganisasiArtikel bersama
Achmad FarajallahIPB University4
Yeni Aryati MulyaniIPB University3
Entang IskandarIPB University3
Andre PasethaIPB University2
Puji RiantiIPB University2
Huda Shalahudin DarusmanIPB University2
Hana Intishar SawitriIPB University2
Tommy Langgeng AbimanyuIPB University2
Muladno MuladnoIPB University2
Maria UlfahIPB University2
Dewi Apri AstutiIPB University1
Indira Nurul QomariahIPB University1
Eka arismayantiIPB University1
Waterman James O.Liverpool John Moores University1
Islamul HadiIPB University1
Bambang SuryobrotoIPB University1
Dondin SajuthiIPB University1
Joko PamungkasNational Research and Innovation Agency1
Alexander NaterUniversity of Zurich1
Michael KrützenIPB University1
Chairunas Adha PutraIPB University1
Muhammad AgilIPB University1
Lisa M. DanishGerman Primate Center1
Antje EngelhardtLiverpool John Moores University1
Sodikin SodikinUniversitas Islam Negeri Raden Intan Lampung1
Riki HerliansyahInstitut Teknologi Kalimantan1
Alexander Kurniawan Sariyanto PuteraIPB University1
Stanislav LhotaCzech University of Life Sciences Prague1
Indra PurnamaIPB University1
Vallen Sakti MaulanaIPB University1
Annisa Devi RachmawatiIPB University1
RizaldiAfiliasi tidak diketahui1
Uus SaepulohIPB University1
Ruhama Maya SariIPB University1
Alfiyan AchmadIPB University1
Achmad AlfiyanIPB University1
Hanny RamadhantiIPB University1
Rika RaffiudinIPB University1
Tiara SayustiIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">DISCRIMINATION OF TWO SPECIES OF ORANGUTANS (Pongo sp.): A RAPID PROTOCOL FOR REHABILITATION CENTRES AND ZOOS</dc:title>
	<dc:creator xml:lang="en">Perwitasari-Farajallah, Dyah</dc:creator>
	<dc:description xml:lang="en">      Currently orangutans exist in increasingly fragmented and isolated populations. While Sumatran orangutan is primarily found in northern Sumatra, the Bornean is distributed in Central, West, and East Kalimantan, Sarawak and Sabah.  The determination of intra- and inter-species variation between Bornean and Sumatran orangutans has been stated to be essential for both the management of orangutan reintroduction projects and the planning of conservation strategies to preserve the remaining wild populations.  This study aimed to identify two species of Orangutans (Pongo sp.) by means of RFLP (Restriction Fragment Length Polymorphisms) analyses of mitochondrial DNA (mtDNA).  An approximately 540 bp single fragment of the ND5 gene near the 5’-region was PCR amplified for all samples tested.  Digestion pattern for both AluI and MseI were different between two groups of ND5 fragments in this study.  Present result showed a rapid protocol to identify these two species by means of RFLP (Restriction Fragment Length Polymorphism) analyses of mtDNA (mitochondrial DNA).  This technique can be applied easily to rehabilitation centres and zoos to resolve species discrimination problem.   Keywords: Orangutans, Pongo sp., Sumatra, Borneo, discrimination</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2024-01-11</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/52</dc:identifier>
	<dc:identifier>10.11598/btb.2009.16.2.52</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 16 No. 2 (2009): BIOTROPIA Vol. 16 No. 2 December 2009</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2009.16.2.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/52/18</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2017 BIOTROPIA - The Southeast Asian Journal of Tropical Biology</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
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