Melola Discovery

← Melola Discovery

Akses terbuka Jurnal SINTA 1

ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE

BIOTROPIA · Vol. 16 No. 1 (2024)

DOI
10.11598/btb.2009.16.1.64
ISSN
1907-770X, 0215-6334
Terbit
2024-01-11
Penerbit
SEAMEO BIOTROP
Bahasa
en
Lisensi
creativecommons.org/licenses/by-nc-nd/4.0

Abstrak

Metallothionein is an important protein for detoxifying heavy metal ions. This research was conducted to isolate and clone cDNA of gene encoding for metallothionein type 2 from Melastoma affine. Total RNA was isolated from young leaves. Total cDNA was synthesized from the total RNA by reverse transcription. The MaMt2 cDNA was successfully isolated by PCR technique. The MaMt2 cDNA was inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into Escherichia coli DH5α. DNA sequencing analysis showed that this cDNA is full length consisting of 246 pb encoding 81 amino acid residues. This cDNA is identical to mRNA of AtMt2 from Arabidopsis thaliana. It does not contain any restriction sites found in the cloning sites of pGEM-T easy. The deduced protein of MaMT2 contains 14 cysteine residues distributed in the Cys-Cys, Cys-X-Cys, and Cys-X-X-Cys motifs.  Key words: cDNA, metallothionein, Melastoma affine, cloning, cysteine

Jaringan kolaborasi para penulis

40 penulis · 99 tautan kepenulisan bersama · 41 lintas organisasi · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • IPB University
  • University of Darussalam Gontor
  • National Research and Innovation Agency
  • Indonesian Institute of Sciences
  • Nara Institute of Science and Technology
  • Sriwijaya University
  • Southeast Asian Regional Centre for Tropical Biology
  • Organisasi lain
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Aris TjahjoleksonoIPB University3
Alimuddin AlimuddinIPB University2
Nampiah SukarnoIPB University2
Latifah Kosim DarusmanIPB University2
Rohani Cinta Badia GintingIPB University2
Miftahudin MiftahudinIPB University2
Didy SopandieIPB University2
Lutfy Ditya CahyantiUniversity of Darussalam Gontor1
Umi IsnatinUniversity of Darussalam Gontor1
Siti Azizah ArdiningtyasUniversity of Darussalam Gontor1
Qaanitatul Hakim IpaulleUniversity of Darussalam Gontor1
Mahmudah HamawiUniversity of Darussalam Gontor1
AlimuddinAfiliasi tidak diketahui1
Mochamad SyaifudinSriwijaya University1
Agus Oman SudrajatIPB University1
Ratu Siti AliahNational Research and Innovation Agency1
Komar SumantadinataIPB University1
Rita MegiaIPB University1
Elizabeth Anita WidjajaIPB University1
Stephanie StephanieAfiliasi tidak diketahui1
Suryo WiyonoIPB University1
Andi ParenrengiNational Research and Innovation Agency1
Tri HandayaniIndonesian Institute of Sciences1
Emma SuryatiNational Research and Innovation Agency1
Erina SulistianiSoutheast Asian Regional Centre for Tropical Biology1
Ence Darmo Jaya SupenaIPB University1
Himmah RustiamiNational Research and Innovation Agency1
Rugayah RugayahIndonesian Institute of Sciences1
Deby ArifianiIPB University1
Deni SahroniNational Research and Innovation Agency1
SuharsonoNational Research and Innovation Agency1
Radite TistamaIPB University1
Akiho YokotaNara Institute of Science and Technology1
Kinya AkashiNara Institute of Science and Technology1
SIHEGIKO KANAYAAfiliasi tidak diketahui1
Wawan Abdullah SetiawanLampung University1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">ISOLATION AND CLONING OF cDNA OF GENE  ENCODING FOR METALLOTHIONEIN TYPE 2 FROM  MELASTOMA AFFINE</dc:title>
	<dc:creator xml:lang="en">SUHARSONO, SUHARSONO</dc:creator>
	<dc:creator xml:lang="en">TRISNANINGRUM, NIKEN</dc:creator>
	<dc:creator xml:lang="en">SULISTYANINGSIH, LULUT DWI</dc:creator>
	<dc:creator xml:lang="en">WIDYASTUTI, UTUT</dc:creator>
	<dc:description xml:lang="en">  Metallothionein is an important protein for detoxifying heavy metal ions. This research was conducted to isolate and clone cDNA of gene encoding for metallothionein type 2 from Melastoma affine. Total RNA was isolated from young leaves. Total cDNA was synthesized from the total RNA by reverse transcription. The MaMt2 cDNA was successfully isolated by PCR technique. The MaMt2 cDNA was inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into Escherichia coli DH5α. DNA sequencing analysis showed that this cDNA is full length consisting of 246 pb encoding 81 amino acid residues. This cDNA is identical to mRNA of AtMt2 from Arabidopsis thaliana. It does not contain any restriction sites found in the cloning sites of pGEM-T easy. The deduced protein of MaMT2 contains 14 cysteine residues distributed in the Cys-Cys, Cys-X-Cys, and Cys-X-X-Cys motifs.   Key words: cDNA, metallothionein, Melastoma affine, cloning, cysteine</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2024-01-11</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/64</dc:identifier>
	<dc:identifier>10.11598/btb.2009.16.1.64</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 16 No. 1 (2009): BIOTROPIA Vol. 16 No. 1 June 2009</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2009.16.1.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/64/43</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2017 BIOTROPIA - The Southeast Asian Journal of Tropical Biology</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
</oai_dc:dc>