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HETEROLOGOUS EXPRESSION OF A CHITINASE GENE FROM AEROMONAS CAVIAEIN PSEUDOMONAS FLUORESCENS

BIOTROPIA No. 20 (2011)

DOI
10.11598/btb.2003.0.20.182
ISSN
1907-770X, 0215-6334
Terbit
2011-11-17
Penerbit
SEAMEO BIOTROP
Bahasa
en

Abstrak

A transcriptional fusion for an Aeromonas caviae chitinase gene was constructed under the control of a constitutive promoter of the kanaraycin resistance gene (PKmR). The construct was inserted into a medium copy number broad host range plasmid vector to yield recombinant plasmid pAM340, which harbored transcriptional fusion PKmR- chi. Another transcriptional fusion, Ptac-chi, in a recombinant plasmid pAM630, was conducted as comparison. Triparental mating of E. coli carrying the recombinant plasmids with Pseudomotws fluorescens 5100, a phyllosphere bacterium, was performed. Pseudomonas fluorescens 5100 exconjugants were examined for constitutive expression of chitinase employing a spectrophotometric assay; they showed stronger chitin degradation activity than Escherichia coli transformants. Using a fungal antagonism plate assay, this chitinolytic P. fluorescens, however, could not inhibit selected phytopathogenic fungi. Keywords:   Aeromonas  caviae/ chitinase  gene/transcriptional  fusion/PKm'V  Vtac-chilPseudomonas fluorescens

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  • IPB University
  • University of Indonesia
  • Scottish Agricultural College
  • Universitas Sumatera Utara
  • Universitas Pattimura
  • Montana State University
  • National Research and Innovation Agency
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Rekan penulisOrganisasiArtikel bersama
Esti PuspitasariIPB University5
İman RusmanaIPB University4
Nisa Rachmania MubarikIPB University4
Dwi SuryantoUniversitas Sumatera Utara3
Anja MeryandiniIPB University3
Aris Tri WahyudiIPB University3
Andreas Adhi SatyaAfiliasi tidak diketahui3
Widanarni WidanarniIPB University2
Bibiana W. LayIPB University2
Yulin LestariIPB University2
Dedy Duryadi SolihinIPB University2
Cecilia Anna SeumahuUniversitas Pattimura2
Maria Indah PurnamasariMontana State University2
Rika Indri AstutiIPB University2
Yepy H. RustamAfiliasi tidak diketahui2
Is HeliantiNational Research and Innovation Agency2
Fairuz Andini FatiningtyasUniversity of Indonesia2
Riswanto NapitupuluUniversity of Indonesia2
Walter R. ErdelenIPB University2
Nurul AiniIPB University1
Muhammad ZairinIPB University1
Ridwan AffandiIPB University1
Yusminah HalaState University of Makassar1
Sukenda SukendaIPB University1
SumardiAfiliasi tidak diketahui1
Maggy T HenawidjajaIPB University1
T Resnawati P UrwadariaAfiliasi tidak diketahui1
Andi KhaeruniUniversitas Halu Oleo1
Budi TjahjonoIPB University1
Meity Suradji SinagaIPB University1
Ari SusilowatiSebelas Maret University1
Suryo WiyonoIPB University1
Rasti SaraswatiAfiliasi tidak diketahui1
Ratih Dewi HastutiIPB University1
. EFRIWATIAfiliasi tidak diketahui1
Gayuh RahayuIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">HETEROLOGOUS EXPRESSION OF A CHITINASE GENE FROM                    AEROMONAS CAVIAEIN PSEUDOMONAS FLUORESCENS</dc:title>
	<dc:creator xml:lang="en">SUWANTO, ANTONIUS</dc:creator>
	<dc:creator xml:lang="en">MALIK, AMARILA</dc:creator>
	<dc:creator xml:lang="en">TJAHJONO, BUDI</dc:creator>
	<dc:creator xml:lang="en">HARLING, ROB</dc:creator>
	<dc:description xml:lang="en">A transcriptional fusion for an Aeromonas caviae chitinase gene was constructed under the control of a constitutive promoter of the kanaraycin resistance gene (PKmR). The construct was inserted into a medium copy number broad host range plasmid vector to yield recombinant plasmid pAM340, which harbored transcriptional fusion PKmR- chi. Another transcriptional fusion, Ptac-chi, in a recombinant plasmid pAM630, was conducted as comparison. Triparental mating of E. coli  carrying the recombinant plasmids with Pseudomotws fluorescens  5100, a phyllosphere bacterium, was performed. Pseudomonas fluorescens  5100 exconjugants were examined for constitutive expression of chitinase employing a spectrophotometric assay; they showed stronger chitin degradation activity than Escherichia coli transformants. Using a fungal antagonism plate assay, this chitinolytic P. fluorescens, however, could not inhibit selected phytopathogenic fungi. Keywords:    Aeromonas   caviae/  chitinase   gene/transcriptional   fusion/PKm'V   Vtac-chilPseudomonas fluorescens</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2011-11-17</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/182</dc:identifier>
	<dc:identifier>10.11598/btb.2003.0.20.182</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; No. 20 (2003)</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2003.0.20.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/182/151</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2017 BIOTROPIA - The Southeast Asian Journal of Tropical Biology</dc:rights>
</oai_dc:dc>