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MOLECULAR IDENTIFICATION OF EGG PARASITOID, TELENOMUS SPP. (HYMENOPTERA: SCELIONIDAE) FROM SEVERAL LOCATIONS IN JAVA USING RAPD-PCR

BIOTROPIA No. 19 (2024)

DOI
10.11598/btb.2002.0.19.226
ISSN
1907-770X, 0215-6334
Terbit
2024-01-11
Penerbit
SEAMEO BIOTROP
Bahasa
en
Lisensi
creativecommons.org/licenses/by-nc-nd/4.0

Abstrak

Random amplified polymorphic DNA (RAPD) amplified by the polymerase chain reaction (PCR) was used to determine the differences of four Telenomus species and five populations of T. rowani from several locations in Java. Amplification of genomic DNA by using primer P2 (Amersham Pharmacia Biotech) indicated that each Telenomus species had a unique set of RAPD bands. Two bands which characterized the genus are estimated to be 300 and 430 bp. Each species had three specific bright bands except T. dignoides which only had two specific bright bands. However, no bands are unique to any of the five populations of T. rowani and all of the bands are less than 500 base-pair. Cluster analysis using UPGMA (Unweighed Pair Group Method With Arithmatic Mean) showed that the four Telenomus species consist of two groups, T. rowani and T. remus in one cluster and T. dignus with T. dignoides belonging to another cluster. Key words: PCR-RAPD / clustering / Telenomus spp.

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  • IPB University
  • Andalas University
  • University of Brawijaya
  • National Research and Innovation Agency
  • University of Vienna
  • Organisasi Riset Pertanian dan Pangan
  • Lampung University
  • Organisasi lain
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Rekan penulisOrganisasiArtikel bersama
Sjafrida ManuwotoIPB University4
Akhmad RizaliUniversity of Brawijaya3
Hermanu TriwidodoIPB University3
Bambang Tri RahardjoUniversity of Brawijaya3
MarwotoOrganisasi Riset Pertanian dan Pangan3
Dewi SartiamiIPB University2
Dedi HutapeaNational Research and Innovation Agency2
Dadang DadangIPB University2
Bandung SahariAfiliasi tidak diketahui2
CHRISTIAN HANSJOACHIM SCHULZEUniversity of Vienna2
Tri AtmowidiIPB University2
Bambang SuryobrotoIPB University2
Pudjianto .Afiliasi tidak diketahui2
Christine WulandariLampung University1
Rinekso SoekmadiIPB University1
Novriyanti NovriyantiIPB University1
Burhanuddin Masy’udIPB University1
Ismanto IsmantoAfiliasi tidak diketahui1
Sri HerizaAndalas University1
Idham Sakti HarahapIPB University1
Nina MaryanaIPB University1
Lutfi AfifahIPB University1
Weerachon SawangprohMahidol University1
Nurindah NurindahAfiliasi tidak diketahui1
Dedy Duryadi SolihinIPB University1
I Wayan SuanaUniversity of Mataram1
Purnama HidayatAfiliasi tidak diketahui1
SYAFRIDA MANUWOTOAfiliasi tidak diketahui1
YAHER WANDIAfiliasi tidak diketahui1
LILIK BUDIPRASETYOAfiliasi tidak diketahui1
Sri Hendrastuti HidayatIPB University1
Noor AidawatiIPB University1
Rusmilah SusenoIPB University1
Sriani SujiprihatiIPB University1
Merijn M. BosLouis Bolk Instituut1
Seiki YamaneKagoshima University1
Endang Sri RatnaIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">MOLECULAR IDENTIFICATION OF EGG PARASITOID, TELENOMUS SPP. (HYMENOPTERA: SCELIONIDAE) FROM SEVERAL LOCATIONS IN JAVA USING RAPD-PCR</dc:title>
	<dc:creator xml:lang="en">YULIARTI, NETTI</dc:creator>
	<dc:creator xml:lang="en">HIDAYAT, PURNAMA</dc:creator>
	<dc:creator xml:lang="en">BUCHORI, DAMAYANTI</dc:creator>
	<dc:description xml:lang="en">Random amplified polymorphic DNA (RAPD) amplified by the polymerase chain reaction (PCR) was used to determine the differences of four Telenomus species and five populations of T. rowani from several locations in Java. Amplification of genomic DNA by using primer P2 (Amersham Pharmacia Biotech) indicated that each Telenomus species had a unique set of RAPD bands. Two bands which characterized the genus are estimated to be 300 and 430 bp. Each species had three specific bright bands except T. dignoides which only had two specific bright bands. However, no bands are unique to any of the five populations of T. rowani and all of the bands are less than 500 base-pair. Cluster analysis using UPGMA (Unweighed Pair Group Method With Arithmatic Mean) showed that the four Telenomus species consist of two groups, T. rowani and T. remus  in one cluster and T. dignus with T. dignoides belonging to another cluster. Key words : PCR-RAPD / clustering / Telenomus spp.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2024-01-11</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/226</dc:identifier>
	<dc:identifier>10.11598/btb.2002.0.19.226</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; No. 19 (2002)</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2002.0.19.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/226/195</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2017 BIOTROPIA - The Southeast Asian Journal of Tropical Biology</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
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