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A SPECIES-SPECIFIC PCR ASSAY BASED ON THE INTERNAL TRANSCRIBED SPACER (ITS) REGIONS FOR IDENTIFICATION OF MYCOSPHAERELLA EUMUSAE, M. FIJIENSIS AND M. MUSICOLA ON BANANA

BIOTROPIA · Vol. 19 No. 1 (2012)

DOI
10.11598/btb.2012.19.1.232
ISSN
1907-770X, 0215-6334
Terbit
2012-11-02
Penerbit
SEAMEO BIOTROP
Bahasa
en

Abstrak

A study on development of a rapid PCR-based detection method based on ITS region of M. eumusae, M. fijiensis, and M. musicola on banana was carried out. The main objecive of this study was to develop a fast and species-specific PCR-based detection method for the presence of Mycosphaerella species on banana. The methods include collection of specimens, morphological identification supported by molecular phylogenetic analysis, RFLP analysis, species-specific primers development, and validation. Two species of Mycosphaerella, namely, M. fijiensis and M. musicola, and one unidentified Pseudocercospora species were found in Java island. Three restriction enzymes used in the RFLP analysis, viz, AluI, HaeIII, and TaqI were capable to discriminate M. eumusae, M. fijiensis, and M. musicola. Two species-specific primer pairs, viz, MfijF/MfijR and MmusF/MmusR have been successfully developed to detect for the presence of M. fijiensis and M. musicola, respectively.

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  • IPB University
  • Indonesian Institute of Sciences
  • Atma Jaya Catholic University of Indonesia
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
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Rekan penulisOrganisasiArtikel bersama
Gayuh RahayuIPB University2
Anastasia Tatik HartantiAtma Jaya Catholic University of Indonesia1
Ivan Permana PutraIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">A SPECIES-SPECIFIC PCR ASSAY BASED ON THE INTERNAL TRANSCRIBED SPACER (ITS) REGIONS FOR IDENTIFICATION OF MYCOSPHAERELLA EUMUSAE, M. FIJIENSIS AND M. MUSICOLA ON BANANA</dc:title>
	<dc:creator xml:lang="en">Hidayat, Iman</dc:creator>
	<dc:description xml:lang="en"> A study on development of a rapid PCR-based detection method based on ITS region of M. eumusae, M. fijiensis, and M. musicola on banana was carried out. The main objecive of this study was to develop a fast and species-specific PCR-based detection method for the presence of Mycosphaerella species on banana. The methods include collection of specimens, morphological identification supported by molecular phylogenetic analysis, RFLP analysis, species-specific primers development, and validation. Two species of Mycosphaerella, namely, M. fijiensis and M. musicola, and one unidentified Pseudocercospora species were found in Java island. Three restriction enzymes used in the RFLP analysis, viz, AluI, HaeIII, and TaqI were capable to discriminate M. eumusae, M. fijiensis, and M. musicola. Two species-specific primer pairs, viz, MfijF/MfijR and MmusF/MmusR have been successfully developed to detect for the presence of M. fijiensis and M. musicola, respectively.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2012-11-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/232</dc:identifier>
	<dc:identifier>10.11598/btb.2012.19.1.232</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 19 No. 1 (2012): BIOTROPIA Vol. 19 No. 1 June 2012</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2012.19.1.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/232/211</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2017 BIOTROPIA - The Southeast Asian Journal of Tropical Biology</dc:rights>
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