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BINARY VECTOR CONSTRUCTION AND Agrobacterium tumefaciens-MEDIATED TRANSFORMATION OF LYSOZYME GENE IN SEAWEED Kappaphycus alvarezii

BIOTROPIA · Vol. 21 No. 2 (2014) · hlm. 82–92

DOI
10.11598/btb.2014.21.2.348
ISSN
1907-770X, 0215-6334
Terbit
2014-11-28
Penerbit
SEAMEO BIOTROP
Bahasa
en
Lisensi
creativecommons.org/licenses/by-nc-nd/4.0

Abstrak

Ice-ice disease is the biggest problem in the cultivation of seaweed Kappaphycus alvarezii. The disease is caused by bacterial infection and induced by drastic changes of water quality. Lysozyme has the ability to break down bacterial cell wall. The purpose of this research was to construct of a binary vector pMSH1-Lys carrying chicken Lysozyme (Lis) gene and introduce pMSH1-Lis on K. alvarezii. The binary vector expression was transformed into Agrobacterium tumefaciens LBA4404 by triparental mating. Thallus was inoculated with A. tumefaciens carrying pMSH1-Lis and then the transformed thallus was selected by adding 20 mg/L hygromycin to the culture medium. PCR analysis showed that the construction of the binary plasmid pMSH1-Lys was established. Percentage of transformation of pMSH1-Lis on K. alvarezii was 23.56%, while the efficiency of regeneration was 11.32%. PCR analysis showed that three of the regenerated thallus contained Lysozyme gene. Thus, transgenic K. alvarezii was produced successfully and this can be useful for studying the mechanisms of seaweed defense against bacterial infection.

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  • State University of Semarang
  • Universitas Halu Oleo
  • Universitas Sulawesi Tenggara
  • Hasanuddin University
  • Diponegoro University
  • Universitas Sulawesi Barat
  • Universitas Airlangga
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Rekan penulisOrganisasiArtikel bersama
Lastuti AbubakarAfiliasi tidak diketahui3
Sri NuryatiAfiliasi tidak diketahui3
Sri KhayatiUniversitas Sulawesi Tenggara2
Edy CahyonoState University of Semarang2
Dante AlighiriState University of Semarang2
Sabila AisyahState University of Semarang2
Bella PermatasariState University of Semarang2
Suharsono SuharsonoAfiliasi tidak diketahui2
Nur Bambang Priyo UtomoAfiliasi tidak diketahui2
Nampiah SukarnoAfiliasi tidak diketahui2
LATIFAH KOSIM DARUSMANAfiliasi tidak diketahui2
ROHANI CINTA BADIA GINTINGAfiliasi tidak diketahui2
SIHEGIKO KANAYAAfiliasi tidak diketahui2
AlimuddinAfiliasi tidak diketahui2
Djohar ArifinUniversitas Sulawesi Tenggara1
Rifkah Anniza RahmanUniversitas Sulawesi Tenggara1
Sri MulyaniDiponegoro University1
Dewi AnggrainiUniversitas Halu Oleo1
Sulsalman MoitaUniversitas Halu Oleo1
Muhammad ArsyadUniversitas Halu Oleo1
Jamaluddin HosUniversitas Halu Oleo1
Wilma J.C. MokaAfiliasi tidak diketahui1
Nita RukminasariHasanuddin University1
Andi Aliah HidayaniHasanuddin University1
Nur Indah Sari ArbitUniversitas Sulawesi Barat1
Sapto AndriyonoUniversitas Airlangga1
Muhammad NadjibAfiliasi tidak diketahui1
Nugrahaningsih WHState University of Semarang1
Saffanah Binti Mohd Ab AzidPrince of Songkla University1
Adrian Maulana ZakyState University of Semarang1
Nanik WijayatiState University of Semarang1
Harjono HarjonoAfiliasi tidak diketahui1
Firmansyah DahlanAfiliasi tidak diketahui1
rasyidah jalilAfiliasi tidak diketahui1
Muh Iqbal HasanuddinAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">BINARY VECTOR CONSTRUCTION AND Agrobacterium tumefaciens-MEDIATED TRANSFORMATION OF LYSOZYME GENE IN SEAWEED Kappaphycus alvarezii</dc:title>
	<dc:creator xml:lang="en">handayani, Tri</dc:creator>
	<dc:creator xml:lang="en">Alimuddin, Alimuddin</dc:creator>
	<dc:creator xml:lang="en">Widyastuti, Utut</dc:creator>
	<dc:creator xml:lang="en">Suryati, Emma</dc:creator>
	<dc:creator xml:lang="en">Parenrengi, Andi</dc:creator>
	<dc:description xml:lang="en">Ice-ice disease is the biggest problem in the cultivation of seaweed Kappaphycus alvarezii. The disease is caused by bacterial infection and induced by drastic changes of water quality. Lysozyme has the ability to break down bacterial cell wall. The purpose of this research was to construct of a binary vector pMSH1-Lys carrying chicken Lysozyme (Lis) gene and introduce pMSH1-Lis on K. alvarezii. The binary vector expression was transformed into Agrobacterium tumefaciens LBA4404 by triparental mating. Thallus was inoculated with A. tumefaciens carrying pMSH1-Lis and then the transformed thallus was selected by adding 20 mg/L hygromycin to the culture medium. PCR analysis showed that the construction of the binary plasmid pMSH1-Lys was established. Percentage of transformation of pMSH1-Lis on K. alvarezii was 23.56%, while the efficiency of regeneration was 11.32%. PCR analysis showed that three of the regenerated thallus contained Lysozyme gene. Thus, transgenic K. alvarezii was produced successfully and this can be useful for studying the mechanisms of seaweed defense against bacterial infection.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2014-11-28</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/348</dc:identifier>
	<dc:identifier>10.11598/btb.2014.21.2.348</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 21 No. 2 (2014): BIOTROPIA Vol. 21 No. 2 December 2014; 82-92</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2014.21.2.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/348/244</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2017 BIOTROPIA - The Southeast Asian Journal of Tropical Biology</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
</oai_dc:dc>