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MOLECULAR CHARACTERIZATION OF BEGOMOVIRUS INFECTING YARD LONG BEAN (Vigna unguiculata SUBSP. sesquipedalis L.) IN JAVA, INDONESIA

BIOTROPIA · Vol. 22 No. 1 (2015) · hlm. 53–60

DOI
10.11598/btb.2015.22.1.401
ISSN
1907-770X, 0215-6334
Terbit
2015-08-23
Penerbit
SEAMEO BIOTROP
Bahasa
en
Lisensi
creativecommons.org/licenses/by-nc-nd/4.0

Abstrak

Begomovirus has been identified as one of the causal agents associated with yellow mosaic disease in yardlong bean (Vigna unguiculata subsp. sesquipedalis (L.)) in Java. Previous studies reported that Begomovirus has infected several Leguminosae species in South Asia, and several Begomoviruses have also been reported to infect important crops in Indonesia based on nucleotide sequence characterization. This study was conducted to identify and characterize Begomovirus isolated from yardlong bean samples in Java based on specific genomic features of the Common Region. Samples were collected from yardlong bean fields in Central Java (Tegal, Magelang, and Klaten), Yogyakarta (Sleman), and West Java (Bogor and Subang); viral detection was performed using I-ELISA, PCR, and sequencing; and molecular characterization was conducted using BioEdit v.7.0.5 and MEGA 6.06 software. Yellow mosaic disease was observed in nearly all surveyed fields, and infection of Potyvirus and Begomovirus was detected either as single or mixed infection. PCR results confirmed Begomovirus infection in samples from Tegal, Klaten, Magelang, Subang, and Bogor. Sequence analysis revealed that the virus infecting yardlong bean was Mungbean Yellow Mosaic India Virus (MYMIV) and shared close genetic similarity with isolates from Bangladesh, India, Pakistan, and Nepal. Further analysis revealed conserved regions surrounding the Common Region, including the “TATA box” sequence, hairpin-loop structure, repetitive sequences, and the conserved nonanucleotide TAATATTAC. This study provides the first report of MYMIV infection in Indonesia.

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  • IPB University
  • Universitas Halu Oleo
  • National Research and Innovation Agency
  • Indonesian Institute of Sciences
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Rekan penulisOrganisasiArtikel bersama
Widodo WidodoIPB University5
Asmar HasanUniversitas Halu Oleo4
SRIANI SUJIPRIHATIAfiliasi tidak diketahui2
Noor AidawatiIPB University2
Sriani SujiprihatiIPB University2
Tri Asmira DamayantiIPB University2
GiyantoNational Research and Innovation Agency2
Suryo WiyonoIPB University2
Muhammad TaufikUniversitas Halu Oleo2
Muhammad TaufikUniversitas Halu Oleo2
Muhamad SyukurIPB University2
Purnama HidayatIPB University2
Sari NurulitaAfiliasi tidak diketahui2
Sofi MawarniIPB University2
Amanda Febriyanti NurdeviIPB University2
Didiet Rahayu DianaAfiliasi tidak diketahui2
Redy GaswantoAfiliasi tidak diketahui1
Bambang Sapta PurwokoIPB University1
MUHAMMAD TAUFIKUniversitas Halu Oleo1
Gede SuastikaAfiliasi tidak diketahui1
SIENTJE MANDANG SUMARAWAfiliasi tidak diketahui1
Sri SulandariIndonesian Institute of Sciences1
RUSMILAH SUSENOAfiliasi tidak diketahui1
JUMANTO HARJOSUDARMOAfiliasi tidak diketahui1
SOEMARTONO SOSROMARSONOAfiliasi tidak diketahui1
Endang NurhayatiAfiliasi tidak diketahui1
DWI SUBEKTIAfiliasi tidak diketahui1
Rusmilah SusenoIPB University1
ORAWAN CHATCHAWANKAN PANICHAfiliasi tidak diketahui1
Hamdayanty HamdayantyAfiliasi tidak diketahui1
Tri Asmira DamayantiAfiliasi tidak diketahui1
Yuliana Ayu LestariAfiliasi tidak diketahui1
Abdjad Asih NawangsihAfiliasi tidak diketahui1
Abdjad Asih NawangsihIPB University1
Siti Halida Hamzah-FZIPB University1
MelindaAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">MOLECULAR CHARACTERIZATION OF BEGOMOVIRUS INFECTING YARD LONG BEAN (Vigna unguiculata SUBSP. sesquipedalis L.) IN JAVA, INDONESIA</dc:title>
	<dc:creator xml:lang="en">Nurulita, Sari</dc:creator>
	<dc:creator xml:lang="en">Hidayat, Sri Hendrastuti</dc:creator>
	<dc:creator xml:lang="en">Mutaqin, Kikin Hamzah</dc:creator>
	<dc:creator xml:lang="en">Thomas, John</dc:creator>
	<dc:subject xml:lang="en">Begomovirus</dc:subject>
	<dc:subject xml:lang="en">Common region</dc:subject>
	<dc:subject xml:lang="en">DNA sequencing</dc:subject>
	<dc:subject xml:lang="en"> I-ELISA</dc:subject>
	<dc:subject xml:lang="en">MYMIV</dc:subject>
	<dc:subject xml:lang="en"> PCR</dc:subject>
	<dc:subject xml:lang="en">Yard long bean</dc:subject>
	<dc:description xml:lang="en">Begomovirus has been identified as one of the causal agents associated with yellow mosaic disease in yardlong bean (Vigna unguiculata subsp. sesquipedalis (L.)) in Java. Previous studies reported that Begomovirus has infected several Leguminosae species in South Asia, and several Begomoviruses have also been reported to infect important crops in Indonesia based on nucleotide sequence characterization. This study was conducted to identify and characterize Begomovirus isolated from yardlong bean samples in Java based on specific genomic features of the Common Region. Samples were collected from yardlong bean fields in Central Java (Tegal, Magelang, and Klaten), Yogyakarta (Sleman), and West Java (Bogor and Subang); viral detection was performed using I-ELISA, PCR, and sequencing; and molecular characterization was conducted using BioEdit v.7.0.5 and MEGA 6.06 software. Yellow mosaic disease was observed in nearly all surveyed fields, and infection of Potyvirus and Begomovirus was detected either as single or mixed infection. PCR results confirmed Begomovirus infection in samples from Tegal, Klaten, Magelang, Subang, and Bogor. Sequence analysis revealed that the virus infecting yardlong bean was Mungbean Yellow Mosaic India Virus (MYMIV) and shared close genetic similarity with isolates from Bangladesh, India, Pakistan, and Nepal. Further analysis revealed conserved regions surrounding the Common Region, including the “TATA box” sequence, hairpin-loop structure, repetitive sequences, and the conserved nonanucleotide TAATATTAC. This study provides the first report of MYMIV infection in Indonesia.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2015-08-23</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/401</dc:identifier>
	<dc:identifier>10.11598/btb.2015.22.1.401</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 22 No. 1 (2015): BIOTROPIA Vol. 22 No. 1 June 2015; 53 - 60</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2015.22.1.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/401/262</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2017 BIOTROPIA - The Southeast Asian Journal of Tropical Biology</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
</oai_dc:dc>