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RESISTANCE AGAINST Aeromonas hydrophila INFECTION AND GROWTH OF SECOND GENERATION (F2) AFRICAN CATFISH (Clarias gariepinus) USING SELECTED MOLECULAR MARKERS

BIOTROPIA · Vol. 25 No. 2 (2018) · hlm. 95–102

DOI
10.11598/btb.2018.25.2.742
ISSN
1907-770X, 0215-6334
Terbit
2018-09-26
Penerbit
SEAMEO BIOTROP
Bahasa
en
Lisensi
creativecommons.org/licenses/by-nc-nd/4.0

Abstrak

Aeromonas hydrophila is a pathogenic bacteria that causes mass mortality in catfish. In previous studies, specific pathogen resistant (SPR), A. hydrophila-resistant African catfish first generation (F1) has been cultivated by marker assisted selection using the major histocompatibility complex (MHC) I as a molecular marker. In this study, growth performance, inheritance of the MHC DNA marker in the second generation (F2) of catfish and disease resistance against A. hydrophila infection were observed. The F2 progenies were produced by crossing F1 fish between themselves. Nursery was performed in 80-L glass aquaria, 4 replications for each cross, at the same initial density, for 2 months of rearing. The results showed that daily growth rate of F2 progenies from the SPR broods was significantly higher than those from broods without the marker. Results of the PCR analysis showed that average number of F2 progenies from SPR broods carrying the MHC marker was about 91% higher than that of control. After the fish reached about 12 cm body length, they were challenged by intramuscularly injecting of 0.1 mL A. hydrophila (LD₅₀: 10⁵ CFU/mL) for 7 days. Results of challenge test showed that survival of F2 offspring from the crosses of SPR broods (77.2%) was about two times higher than those from brood without MHC marker (38.3%). Differential leukocyte count supported the high resistance of F2 progenies from F1 broods having MHC I marker against A. hydrophila infection. In conclusion, African catfish farming carrying MHC marker potentially have higher productivity and reduces fish loss due to infection by A. hydrophila.

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  • IPB University
  • Hasanuddin University
  • National Research and Innovation Agency
  • Universitas Gadjah Mada
  • Ministry of Marine Affairs and Fisheries
  • Universitas Airlangga
  • Indonesia Open University
  • Organisasi lain
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Rekan penulisOrganisasiArtikel bersama
Munti YuhanaIPB University4
Nurly FaridahMinistry of Marine Affairs and Fisheries3
Utut WidyastutiIPB University2
Nur Bambang Priyo UtomoIPB University2
Widya AsmaraUniversitas Gadjah Mada2
Jaka WidadaUniversitas Gadjah Mada2
Widanarni WidanarniIPB University2
Mohammad Faizal UlkhaqUniversitas Airlangga2
Mia SetiawatiIPB University2
Irzal EffendiIPB University2
Hasan NasrullahIPB University2
Andi Aliah HidayaniHasanuddin University1
Dody Dh. TrijunoHasanuddin University1
Yushinta FujayaHasanuddin University1
Basse Siang ParawansaHasanuddin University1
Moh. Tauhid UmarHasanuddin University1
Irmawati IrmawatiHasanuddin University1
Aidah Ambo Ala HusainHasanuddin University1
Asmi Citra Malina A.R. TassakkaHasanuddin University1
Nadiarti NadiartiIndonesia Open University1
Andi ParenrengiNational Research and Innovation Agency1
Tri HandayaniIndonesian Institute of Sciences1
Emma SuryatiNational Research and Innovation Agency1
Reni Agustina LubisIPB University1
Dewi YuniatiIPB University1
Shella MarlindaIPB University1
Anna OctaveraIPB University1
Harits Atika AriyatnaNational Research and Innovation Agency1
Sulasy RohmyIPB University1
Shavika MirantiUniversitas Maritim Raja Ali Haji1
AlimuddinIPB University1
AlimuddinAfiliasi tidak diketahui1
. KurdiantoIPB University1
Goro YoshizakiTokyo University of Marine Science and Technology1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">RESISTANCE AGAINST Aeromonas hydrophila INFECTION AND GROWTH OF SECOND GENERATION (F2) AFRICAN CATFISH (Clarias gariepinus) USING SELECTED MOLECULAR MARKERS</dc:title>
	<dc:creator xml:lang="en">Alimuddin, Alimuddin</dc:creator>
	<dc:creator xml:lang="en">Putri, Fadhila Maharani</dc:creator>
	<dc:creator xml:lang="en">Wahjuningrum, Dinamella</dc:creator>
	<dc:creator xml:lang="en">Hardiantho, Dian</dc:creator>
	<dc:creator xml:lang="en">Sunarma, Ade</dc:creator>
	<dc:creator xml:lang="en">Nuryati, Sri</dc:creator>
	<dc:subject xml:lang="en">Aeromonas hydrophila</dc:subject>
	<dc:subject xml:lang="en">African catfish</dc:subject>
	<dc:subject xml:lang="en">disease resistance</dc:subject>
	<dc:subject xml:lang="en">growth</dc:subject>
	<dc:subject xml:lang="en">MHC I</dc:subject>
	<dc:description xml:lang="en">Aeromonas hydrophila is a pathogenic bacteria that causes mass mortality in catfish. In previous studies, specific pathogen resistant (SPR), A. hydrophila-resistant African catfish first generation (F1) has been cultivated by marker assisted selection using the major histocompatibility complex (MHC) I as a molecular marker. In this study, growth performance, inheritance of the MHC DNA marker in the second generation (F2) of catfish and disease resistance against A. hydrophila infection were observed. The F2 progenies were produced by crossing F1 fish between themselves. Nursery was performed in 80-L glass aquaria, 4 replications for each cross, at the same initial density, for 2 months of rearing. The results showed that daily growth rate of F2 progenies from the SPR broods was significantly higher than those from broods without the marker. Results of the PCR analysis showed that average number of F2 progenies from SPR broods carrying the MHC marker was about 91% higher than that of control. After the fish reached about 12 cm body length, they were challenged by intramuscularly injecting of 0.1 mL A. hydrophila (LD₅₀: 10⁵ CFU/mL) for 7 days. Results of challenge test showed that survival of F2 offspring from the crosses of SPR broods (77.2%) was about two times higher than those from brood without MHC marker (38.3%). Differential leukocyte count supported the high resistance of F2 progenies from F1 broods having MHC I marker against A. hydrophila infection. In conclusion, African catfish farming carrying MHC marker potentially have higher productivity and reduces fish loss due to infection by A. hydrophila.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2018-09-26</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/742</dc:identifier>
	<dc:identifier>10.11598/btb.2018.25.2.742</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 25 No. 2 (2018): BIOTROPIA Vol. 25 No. 2 August 2018; 95 - 102</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2018.25.2.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/742/405</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2018 BIOTROPIA - The Southeast Asian Journal of Tropical Biology</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
</oai_dc:dc>