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ISOLATION, CHARACTERIZATION AND MOLECULAR IDENTIFICATION OF MYXOBACTERIA FROM TWO OUTERMOST ISLANDS OF INDONESIA

BIOTROPIA · Vol. 25 No. 2 (2018) · hlm. 121–129

DOI
10.11598/btb.2018.25.2.796
ISSN
1907-770X, 0215-6334
Terbit
2018-09-26
Penerbit
SEAMEO BIOTROP
Bahasa
en
Lisensi
creativecommons.org/licenses/by-nc-nd/4.0

Abstrak

Myxobacteria are Gram negative bacteria commonly found in soil, tree bark, and decay wood. These bacteria have unique social behaviors by forming fruiting bodies, moving by gliding motility and preying on other microorganisms. The research was conducted to isolate, characterize, and identify indigenous myxobacteria from Sumba and Papua Islands of Indonesia as a preliminary step to utilize their potential in the pharmaceutical industry. Myxobacteria were isolated using filter paper and baiting with Escherichia coli to obtain cellulolytic and bacteriolytic myxobacteria, respectively. Characterization of myxobacteria was performed with Gram staining, observation on pigmentation, morphology of vegetative cells, fruiting bodies, and myxospores. Molecular identification was conducted based on 16S rRNA gene sequence analysis. A total of 10 myxobacterial strains were successfully isolated and purified. All isolates obtained were Gram negative, rod shaped with yellow or orange pigmentation. Fruiting bodies observed contained spherical myxospores. Molecular identification of these bacterial strains showed that they belong to myxobacteria from suborder Cystobacterineae, namely Myxococcus fulvus, Myxococcus stipitatus, and Melittangium lichenicola. To our knowledge, this is the first record of their occurrence in Indonesia.

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  • Indonesian Institute of Sciences
  • Universitas Muhammadiyah Semarang
  • Ministry of Marine Affairs and Fisheries
  • Universitas Gadjah Mada
  • Toronto General Hospital
  • IPB University
  • University of Yamanashi
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Rekan penulisOrganisasiArtikel bersama
Tri Ratna SulistiyaniIndonesian Institute of Sciences3
I Made SudianaIndonesian Institute of Sciences2
Atit KantiIndonesian Institute of Sciences2
Masaru KobayashiKyoto University2
Ratih Dwi HastutiAfiliasi tidak diketahui1
Yantyati WidyastutiIndonesian Institute of Sciences1
Yulin LestariIPB University1
Shanti RatnakomalaIndonesian Institute of Sciences1
Nita Rosalinda PrayitnoToronto General Hospital1
Evi TrianaIndonesian Institute of Sciences1
Misa OtoguroUniversity of Yamanashi1
Katsuhiko AndoNational Institute of Technology and Evaluation1
Endang SukaraIndonesian Institute of Sciences1
Dewi Seswita ZildaMinistry of Marine Affairs and Fisheries1
Agustinus R. UriaHokkaido University1
Yudi KusnadiMinistry of Marine Affairs and Fisheries1
Anissa UtamiPancasila University1
Pamela AprilianaIndonesian Institute of Sciences1
Zidny IlmiahIndonesian Institute of Sciences1
Siswa SetyahadiBadan Pengkajian dan Penerapan Teknologi1
Tri WibawaUniversitas Gadjah Mada1
Maya Dian RakhmawatieUniversitas Muhammadiyah Semarang1
Mustofa MustofaUniversitas Gadjah Mada1
Kanti RatnaningrumUniversitas Muhammadiyah Semarang1
Muhammad Mucharom Chairul UmamUniversitas Muhammadiyah Semarang1
Muhammad Hasan AlfiUniversitas Muhammadiyah Semarang1
Listya ChaririUniversitas Muhammadiyah Semarang1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">ISOLATION, CHARACTERIZATION AND MOLECULAR IDENTIFICATION OF MYXOBACTERIA FROM TWO OUTERMOST ISLANDS OF INDONESIA</dc:title>
	<dc:creator xml:lang="en">Meliah, Siti</dc:creator>
	<dc:creator xml:lang="en">Lisdiyanti, Puspita</dc:creator>
	<dc:subject xml:lang="en">characterization</dc:subject>
	<dc:subject xml:lang="en">identification</dc:subject>
	<dc:subject xml:lang="en">isolation</dc:subject>
	<dc:subject xml:lang="en">myxobacteria</dc:subject>
	<dc:description xml:lang="en">Myxobacteria are Gram negative bacteria commonly found in soil, tree bark, and decay wood. These bacteria have unique social behaviors by forming fruiting bodies, moving by gliding motility and preying on other microorganisms. The research was conducted to isolate, characterize, and identify indigenous myxobacteria from Sumba and Papua Islands of Indonesia as a preliminary step to utilize their potential in the pharmaceutical industry. Myxobacteria were isolated using filter paper and baiting with Escherichia coli to obtain cellulolytic and bacteriolytic myxobacteria, respectively. Characterization of myxobacteria was performed with Gram staining, observation on pigmentation, morphology of vegetative cells, fruiting bodies, and myxospores. Molecular identification was conducted based on 16S rRNA gene sequence analysis. A total of 10 myxobacterial strains were successfully isolated and purified. All isolates obtained were Gram negative, rod shaped with yellow or orange pigmentation. Fruiting bodies observed contained spherical myxospores. Molecular identification of these bacterial strains showed that they belong to myxobacteria from suborder Cystobacterineae, namely Myxococcus fulvus, Myxococcus stipitatus, and Melittangium lichenicola. To our knowledge, this is the first record of their occurrence in Indonesia.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2018-09-26</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/796</dc:identifier>
	<dc:identifier>10.11598/btb.2018.25.2.796</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 25 No. 2 (2018): BIOTROPIA Vol. 25 No. 2 August 2018; 121 - 129</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2018.25.2.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/796/408</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2018 BIOTROPIA - The Southeast Asian Journal of Tropical Biology</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
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