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MOLECULAR CLONING OF THE VITELLOGENIN GENE IN THE HARD-LIPPED BARB (Osteochillus hasseltii C.V.) AND PHOTOPERIOD’S EFFECTS ON GENE EXPRESSION

BIOTROPIA · Vol. 25 No. 3 (2018) · hlm. 224–232

DOI
10.11598/btb.2018.25.3.876
ISSN
1907-770X, 0215-6334
Terbit
2018-09-26
Penerbit
SEAMEO BIOTROP
Bahasa
en
Lisensi
creativecommons.org/licenses/by-nc-nd/4.0

Abstrak

Photoperiod affects fish reproduction as it regulates activities of the endocrine glands, which produce the hormones needed for geonadal growth and development, gametogenesis, and reproductive cycles. This study aimed to determine the effects of photoperiod on the hard-lipped barb's reproductive performance by exposing the fish to three photoperiod treatments (light hour: L, darks hour: D), namely 14L:10D (control), 8L:16D (short photoperiod) and 18L:6D (long photoperiod), with four aquaria, each containing 9 fish, serving as replicates. The fish were kept under these photoperiods for 8 weeks. Liver activity, the observable variable in the study, was evaluated by measuring vitellogenin gene expression. Normalized data were then subjected to ANOVA, followed by Tukey's range test. The hard-lipped barb's vitellogenin cDNA was found have a 1136 bp sequence and the vitellogenin precursors encoded cDNA comprising 378 amino acids. The vitellogenin gene in each experimental group saw a significant increase on average when exposed to longer photoperiods (P<0.05), and the highest levels of vitellogenin gene expression occurred under long photoperiods (LP, 18 h light:6 h dark). These results indicate that longer photoperiods stimulate and improve the hard-lipped barb's reproductive performance.

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">MOLECULAR CLONING OF THE VITELLOGENIN GENE IN THE HARD-LIPPED BARB (Osteochillus hasseltii C.V.) AND PHOTOPERIOD’S EFFECTS ON GENE EXPRESSION</dc:title>
	<dc:creator xml:lang="en">Prayogo, Norman Arie</dc:creator>
	<dc:creator xml:lang="en">Siregar, Asrul Sahri</dc:creator>
	<dc:creator xml:lang="en">Sukardi, Purnama</dc:creator>
	<dc:creator xml:lang="en">Bessho, Yasumasa</dc:creator>
	<dc:subject xml:lang="en">amino acid</dc:subject>
	<dc:subject xml:lang="en">cDNA</dc:subject>
	<dc:subject xml:lang="en">Hard-lipped barb</dc:subject>
	<dc:subject xml:lang="en">photoperiods</dc:subject>
	<dc:subject xml:lang="en">vitellogenin</dc:subject>
	<dc:description xml:lang="en">Photoperiod affects fish reproduction as it regulates activities of the endocrine glands, which produce the hormones needed for geonadal growth and development, gametogenesis, and reproductive cycles. This study aimed to determine the effects of photoperiod on the hard-lipped barb's reproductive performance by exposing the fish to three photoperiod treatments (light hour: L, darks hour: D), namely 14L:10D (control), 8L:16D (short photoperiod) and 18L:6D (long photoperiod), with four aquaria, each containing 9 fish, serving as replicates. The fish were kept under these photoperiods for 8 weeks. Liver activity, the observable variable in the study, was evaluated by measuring vitellogenin gene expression. Normalized data were then subjected to ANOVA, followed by Tukey's range test. The hard-lipped barb's vitellogenin cDNA was found have a 1136 bp sequence and the vitellogenin precursors encoded cDNA comprising 378 amino acids. The vitellogenin gene in each experimental group saw a significant increase on average when exposed to longer photoperiods (P&amp;lt;0.05), and the highest levels of vitellogenin gene expression occurred under long photoperiods (LP, 18 h light:6 h dark). These results indicate that longer photoperiods stimulate and improve the hard-lipped barb's reproductive performance.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2018-09-26</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/876</dc:identifier>
	<dc:identifier>10.11598/btb.2018.25.3.876</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 25 No. 3 (2018): BIOTROPIA Vol. 25 No. 3 December 2018; 224 - 232</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2018.25.3.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/876/424</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2018 BIOTROPIA - The Southeast Asian Journal of Tropical Biology</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
</oai_dc:dc>