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PREVALENCE OF mcr-1 COLISTIN-RESISTANT GENE IN Escherichia coli ALONG THE BROILER MEAT SUPPLY CHAIN IN INDONESIA

BIOTROPIA · Vol. 26 No. 2 (2019) · hlm. 143–153

DOI
10.11598/btb.2019.26.2.1054
ISSN
1907-770X, 0215-6334
Terbit
2019-01-24
Penerbit
SEAMEO BIOTROP
Bahasa
en
Lisensi
creativecommons.org/licenses/by-nc-nd/4.0

Abstrak

Colistin is the last drug of choice for dealing with carbapenem-resistant Enterobacteriaceae bacteria; hence, this drug is very crucial to human health. The discovery of a plasmid-mediated colistin-resistant gene, the mobilized colistin resistance-1 (mcr-1), signals a significant global health threat. Colistin sulfate is an antimicrobial agent which has been approved for use in broilers in Indonesia. Thus, this study aimed to measure the prevalence of colistin-resistant Escherichia coli and to detect the mcr-1 colistin-resistant gene in E. coli and E. coli O157:H7 in the entire supply chain of broilers in Bogor Regency, West Java Province, Indonesia. Samples were taken from 47 flocks that used colistin sulfate (47 pools of cloacal swabs, 47 pools of drinking water, and 47 pools of litter), seventy fresh meat samples and seven plucker swab samples from seven small-scale poultry slaughterhouses, seventy fresh meat samples from seven traditional markets, and seventy cooked meat samples from seven small restaurants. The isolation of E. coli was done on each of the 358 samples, and 493 isolates were obtained. All the E. coli isolates were then tested for their susceptibility to colistin sulfate by using the agar dilution method. The detection of the mcr-1 gene from the colistin-resistant isolates (minimum inhibitory concentration > 2 µg/mL) was conducted using the polymerase chain reaction (PCR). The prevalence value of colistin-resistant E. coli in all the isolates was 11.76% (CI 95%; CL 9.21–14.91), and the prevalence of the mcr-1 gene was 10.55% (CI 95%; CL 8.13–13.57%). A very good agreement correlation existed between the colistin-resistant phenotype and the mcr-1 gene (κ = 0.939). The mcr-1 gene was found in 89.66% of colistin-resistant E. coli isolates. Two colistin-resistant and mcr-1 carrying isolates were identified as E. coli O157:H7 serotype. This research was the first study attempt on the mcr-1 gene in Indonesia, covering the entire supply chain of broiler meat from farms to consumers. The results indicated the necessity to reduce the use of colistin sulfate in broiler management and to improve biosecurity measures, not only in farms but also in the entire supply chain of broiler meat production.

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  • IPB University
  • Universitas Syiah Kuala
  • University of Indonesia
  • Ministry of Agriculture
  • Lambung Mangkurat University
  • Universitas Islam Negeri Alauddin Makassar
  • Indonesian Institute of Sciences
  • Organisasi lain
  • Afiliasi tidak diketahui
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Rekan penulisOrganisasiArtikel bersama
Muhammad RizalLambung Mangkurat University4
HERDISAfiliasi tidak diketahui3
Arief BoedionoIPB University3
Ekowati HandharyaniIPB University2
Evy DamayanthiIPB University2
Hadi RiyadiIPB University2
Dwi Santy DamayatiUniversitas Islam Negeri Alauddin Makassar2
Yulnawati YulnawatiIndonesian Institute of Sciences2
. YulnawatiAfiliasi tidak diketahui2
Entang IskandarIPB University2
Dyah Perwitasari‐FarajallahIPB University2
Hana Intishar SawitriIPB University2
Tommy Langgeng AbimanyuIPB University2
. YulianaAfiliasi tidak diketahui2
Irma Herawati SupartoIPB University2
Uus SaepulohIPB University2
Silmi MariyaIPB University2
Muharam SaepullohAfiliasi tidak diketahui2
Cece SumantriIPB University2
Aryani Sismin SatyaningtijasIPB University2
Wasmen ManaluIPB University2
SafikaAfiliasi tidak diketahui1
Aprilia HardiatiIPB University1
Fachriyan Hasmi PasaribuIPB University1
Boky Jeanne TuasikalNational Nuclear Energy Agency of Indonesia1
Sri EstuningsihIPB University1
Umi CahyaningsihIPB University1
Isrok Malikus SufiAfiliasi tidak diketahui1
Basri A. GaniUniversitas Syiah Kuala1
Boy Muchlis BachtiarUniversity of Indonesia1
Santi ChismirinaUniversitas Syiah Kuala1
Zinatul HayatiUniversitas Syiah Kuala1
Endang Winiati BUniversity of Indonesia1
Muhammad GunawanNational Research and Innovation Agency1
Agik SuprayogiIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">PREVALENCE OF mcr-1 COLISTIN-RESISTANT GENE IN Escherichia coli ALONG THE BROILER MEAT SUPPLY CHAIN IN INDONESIA</dc:title>
	<dc:creator xml:lang="en">Palupi, Maria Fatima</dc:creator>
	<dc:creator xml:lang="en">Wibawan, I Wayan Teguh</dc:creator>
	<dc:creator xml:lang="en">Sudarnika, Etih</dc:creator>
	<dc:creator xml:lang="en">Maheshwari, Hera</dc:creator>
	<dc:creator xml:lang="en">Darusman, Huda Shalahudin</dc:creator>
	<dc:subject xml:lang="en">broiler</dc:subject>
	<dc:subject xml:lang="en">colistin</dc:subject>
	<dc:subject xml:lang="en">Escherichia coli</dc:subject>
	<dc:subject xml:lang="en">mcr-1</dc:subject>
	<dc:subject xml:lang="en">supply chain</dc:subject>
	<dc:description xml:lang="en">Colistin is the last drug of choice for dealing with carbapenem-resistant Enterobacteriaceae bacteria; hence, this drug is very crucial to human health. The discovery of a plasmid-mediated colistin-resistant gene, the mobilized colistin resistance-1 (mcr-1), signals a significant global health threat. Colistin sulfate is an antimicrobial agent which has been approved for use in broilers in Indonesia. Thus, this study aimed to measure the prevalence of colistin-resistant Escherichia coli and to detect the mcr-1 colistin-resistant gene in E. coli and E. coli O157:H7 in the entire supply chain of broilers in Bogor Regency, West Java Province, Indonesia. Samples were taken from 47 flocks that used colistin sulfate (47 pools of cloacal swabs, 47 pools of drinking water, and 47 pools of litter), seventy fresh meat samples and seven plucker swab samples from seven small-scale poultry slaughterhouses, seventy fresh meat samples from seven traditional markets, and seventy cooked meat samples from seven small restaurants. The isolation of E. coli was done on each of the 358 samples, and 493 isolates were obtained. All the E. coli isolates were then tested for their susceptibility to colistin sulfate by using the agar dilution method. The detection of the mcr-1 gene from the colistin-resistant isolates (minimum inhibitory concentration &amp;gt; 2 µg/mL) was conducted using the polymerase chain reaction (PCR). The prevalence value of colistin-resistant E. coli in all the isolates was 11.76% (CI 95%; CL 9.21–14.91), and the prevalence of the mcr-1 gene was 10.55% (CI 95%; CL 8.13–13.57%). A very good agreement correlation existed between the colistin-resistant phenotype and the mcr-1 gene (κ = 0.939). The mcr-1 gene was found in 89.66% of colistin-resistant E. coli isolates. Two colistin-resistant and mcr-1 carrying isolates were identified as E. coli O157:H7 serotype. This research was the first study attempt on the mcr-1 gene in Indonesia, covering the entire supply chain of broiler meat from farms to consumers. The results indicated the necessity to reduce the use of colistin sulfate in broiler management and to improve biosecurity measures, not only in farms but also in the entire supply chain of broiler meat production.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2019-01-24</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/1054</dc:identifier>
	<dc:identifier>10.11598/btb.2019.26.2.1054</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 26 No. 2 (2019): BIOTROPIA Vol. 26 No. 2 August 2019; 143 - 153</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2019.26.2.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/1054/486</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2019 BIOTROPIA - The Southeast Asian Journal of Tropical Biology</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
</oai_dc:dc>