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GENETIC IDENTIFICATION OF SEA HOLLY (Acanthus ilicifolius) THROUGH DNA BARCODING FROM COASTAL CILACAP, CENTRAL JAVA, INDONESIA

BIOTROPIA · Vol. 27 No. 1 (2019) · hlm. 60–68

DOI
10.11598/btb.2020.27.1.1105
ISSN
1907-770X, 0215-6334
Terbit
2019-08-28
Penerbit
SEAMEO BIOTROP
Bahasa
en
Lisensi
creativecommons.org/licenses/by-nc-nd/4.0

Abstrak

Sea holly (Acanthus ilicifolius) is an important true mangrove species commonly growing in wetlands at river mouths and coastal areas. Very limited information is available on the molecular taxonomy of sea holly growing along the coasts of Cilacap, Central Java, Indonesia. The present study aimed to identify sea holly in coastal Cilacap and to produce a reference library on the molecular characteristics of the species. The recently recorded species were utilized for the barcoding investigation. Genetic identification was evaluated using the rbcL and matK genes. Young leaf samples of A. ilicifolius were collected for DNA extraction, isolation, and amplification using rbcL and matK primers. The length of the rbcL gene was 608 bp and the matK gene was 970 bp. The evolutionary history was constructed using the Neighbor-Joining method. The barcode sequences of rbcL and matK were analyzed using BLAST and MULTALIN. The sequences were also submitted to NCBI. Genus Acanthus (Acanthaceae) and other genera were clustered in the same clade with high bootstrap values. The results indicated that the rbcL and matK loci cannot be used for species differentiation within Acanthus; however, these genes can be used to distinguish the genus level within Acanthaceae.

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  • Jenderal Soedirman University
  • Diponegoro University
  • Nara Institute of Science and Technology
  • University of Bahri
  • Nasional University
  • University of Brawijaya
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
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Rekan penulisOrganisasiArtikel bersama
Asrul Sahri SiregarJenderal Soedirman University5
Endang HilmiJenderal Soedirman University5
Noprie YansahAfiliasi tidak diketahui3
Tjahyo WinantoJenderal Soedirman University3
Sri MarnaniDiponegoro University3
Agung SudaryonoDiponegoro University3
Arif MahdianaJenderal Soedirman University2
Teuku JunaidiJenderal Soedirman University2
Rose DewiJenderal Soedirman University2
Yasumasa BesshoNara Institute of Science and Technology2
Taufik Budi PramonoAfiliasi tidak diketahui2
Masashi KawaichiNara Institute of Science and Technology2
‪Agus NuryantoJenderal Soedirman University2
Rita Eka RositaJenderal Soedirman University2
Hamdan SyakuriJenderal Soedirman University2
Hanan Hassan Alsheikh MahmoudUniversity of Bahri1
Petrus Hary Tjahja SoedibyaJenderal Soedirman University1
Isdy SulystioJenderal Soedirman University1
Eko Setio WibowoJenderal Soedirman University1
Edy YuwonoNasional University1
Tri Nur CahyoJenderal Soedirman University1
Anjalli Tasha ArdiyantoJenderal Soedirman University1
Sri RahayuUniversity of Brawijaya1
Rizqi Rizaldi HidayatJenderal Soedirman University1
Zahra Safira AuliaDiponegoro University1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">GENETIC IDENTIFICATION OF SEA HOLLY (Acanthus ilicifolius) THROUGH DNA BARCODING FROM COASTAL CILACAP, CENTRAL JAVA, INDONESIA</dc:title>
	<dc:creator xml:lang="en">Harisam, Taufan</dc:creator>
	<dc:creator xml:lang="en">Haryono, Florencius Eko Dwi</dc:creator>
	<dc:creator xml:lang="en">Marhaeni, Bintang</dc:creator>
	<dc:creator xml:lang="en">Amron, Amron</dc:creator>
	<dc:creator xml:lang="en">Prayogo, Norman Arie</dc:creator>
	<dc:creator xml:lang="en">Sukardi, Purnama</dc:creator>
	<dc:subject xml:lang="en">A. ilicifolius</dc:subject>
	<dc:subject xml:lang="en">DNA barcode</dc:subject>
	<dc:subject xml:lang="en">matK</dc:subject>
	<dc:subject xml:lang="en">rbcL</dc:subject>
	<dc:subject xml:lang="en">sea holly</dc:subject>
	<dc:description xml:lang="en">Sea holly (Acanthus ilicifolius) is an important true mangrove species commonly growing in wetlands at river mouths and coastal areas. Very limited information is available on the molecular taxonomy of sea holly growing along the coasts of Cilacap, Central Java, Indonesia. The present study aimed to identify sea holly in coastal Cilacap and to produce a reference library on the molecular characteristics of the species. The recently recorded species were utilized for the barcoding investigation. Genetic identification was evaluated using the rbcL and matK genes. Young leaf samples of A. ilicifolius were collected for DNA extraction, isolation, and amplification using rbcL and matK primers. The length of the rbcL gene was 608 bp and the matK gene was 970 bp. The evolutionary history was constructed using the Neighbor-Joining method. The barcode sequences of rbcL and matK were analyzed using BLAST and MULTALIN. The sequences were also submitted to NCBI. Genus Acanthus (Acanthaceae) and other genera were clustered in the same clade with high bootstrap values. The results indicated that the rbcL and matK loci cannot be used for species differentiation within Acanthus; however, these genes can be used to distinguish the genus level within Acanthaceae.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2019-08-28</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/1105</dc:identifier>
	<dc:identifier>10.11598/btb.2020.27.1.1105</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 27 No. 1 (2020): BIOTROPIA Vol. 27 No. 1 April 2020; 60 - 68</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2020.27.1.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/1105/587</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2019 BIOTROPIA - The Southeast Asian Journal of Tropical Biology</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
</oai_dc:dc>