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PRODUCTION OPTIMIZATION, PARTIAL PURIFICATION, AND THROMBOLYTIC ACTIVITY EVALUATION OF PROTEASE OF Bacillus cereus HSFI-10

BIOTROPIA · Vol. 30 No. 2 (2023) · hlm. 147–157

DOI
10.11598/btb.2023.30.2.1765
ISSN
1907-770X, 0215-6334
Terbit
2023-08-01
Penerbit
SEAMEO BIOTROP
Bahasa
en
Lisensi
creativecommons.org/licenses/by-nc-nd/4.0

Abstrak

Cardiovascular disease is the primary cause of mortality in the world due to the formation of blood clots or thrombi in blood vessels. Bacterial proteases commonly function as thrombus dissolver agents in the pharmaceutical industry. Bacterial isolate HSFI-10 (Holothuria scabra Fermented Intestine-10) previously isolated from Rusip fermented sea cucumber had demonstrated thrombolytic activity. This study aimed to produce crude protease of HSFI-10 strain at an optimized incubation time and determine the thrombolytic activity of crude and dialysate proteases on A, B, AB, and O blood types. Isolate HSFI-10 was first molecularly identified and found to be Bacillus cereus with a homology level of 99.80% with Bacillus cereus strain ST06. The optimum crude enzyme was obtained after 48-h incubation with an activity of 222.52 U/mL, which increased to 438.84 U/mL after ammonium sulfate precipitation and dialysis. Clot lysis activity of crude enzymes was measured based on the gravimetry method on blood in the ABO system, showing results that ranged from 68.99% to 69.76%, while the dialysate ranged from 81.16% to 82.52%. In conclusion, partial purification of bacterial protease could increase both its specific and thrombolytic activities on human blood in the ABO system, with only 1% activity variability between A, B, AB, and O blood types.

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  • Universitas Muhammadiyah Semarang
  • Universitas Gadjah Mada
  • Indonesian Institute of Sciences
  • Ministry of Marine Affairs and Fisheries
  • Pancasila University
  • Hokkaido University
  • Badan Pengkajian dan Penerapan Teknologi
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Rekan penulisOrganisasiArtikel bersama
Agustinus R. UriaHokkaido University2
Yudi KusnadiMinistry of Marine Affairs and Fisheries2
Siswa SetyahadiBadan Pengkajian dan Penerapan Teknologi2
Widya AsmaraUniversitas Gadjah Mada2
Wayan Tunas ArtamaUniversitas Gadjah Mada2
Langkah SembiringUniversitas Gadjah Mada2
Jaka WidadaUniversitas Gadjah Mada1
Tri Joko RaharjoState University of Semarang1
Endang SemiartiUniversitas Gadjah Mada1
Oedjijono OedjijonoJenderal Soedirman University1
Feliatra FelixAfiliasi tidak diketahui1
Tri Aryono HadiIndonesian Institute of Sciences1
Nia M. KurniaPancasila University1
Lidia DinawatiRiau University1
Budi Setiadi DaryonoUniversitas Gadjah Mada1
Endah RetnaningrumUniversitas Gadjah Mada1
Muhammad Evy PrastiyantoUniversitas Gadjah Mada1
Syaiful AnwarDiponegoro University1
Masashi KawaichiNara Institute of Science and Technology1
Puspita LisdiyantiIndonesian Institute of Sciences1
Anissa UtamiPancasila University1
Pamela AprilianaIndonesian Institute of Sciences1
Zidny IlmiahIndonesian Institute of Sciences1
Aprilia Indra KartikaUniversitas Muhammadiyah Semarang1
Hapsari Sulistya KusumaUniversitas Muhammadiyah Semarang1
Mudyawati KamaruddinAkademi Kebidanan Tahirah Al Baeti Bulukumba1
Nastiti WijayantiUniversitas Gadjah Mada1
Sulaiman Ngongu DepamedeUniversity of Mataram1
Maya Tamara MawardaniUniversitas Muhammadiyah Semarang1
Yunita AldirahayuUniversitas Muhammadiyah Semarang1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">PRODUCTION OPTIMIZATION, PARTIAL PURIFICATION, AND THROMBOLYTIC ACTIVITY EVALUATION OF PROTEASE OF Bacillus cereus HSFI-10</dc:title>
	<dc:creator xml:lang="en">Ainutajriani, Ainutajriani</dc:creator>
	<dc:creator xml:lang="en">Darmawati, Sri</dc:creator>
	<dc:creator xml:lang="en">Zilda, Dewi Seswita</dc:creator>
	<dc:creator xml:lang="en">Afriansyah, Muhammad Ardi</dc:creator>
	<dc:creator xml:lang="en">Saptaningtyas, Ragil</dc:creator>
	<dc:creator xml:lang="en">Ethica, Stalis Norma</dc:creator>
	<dc:subject xml:lang="en">Bacillus cereus HSFI-10</dc:subject>
	<dc:subject xml:lang="en">blood system</dc:subject>
	<dc:subject xml:lang="en">clot lysis</dc:subject>
	<dc:subject xml:lang="en">partial purification</dc:subject>
	<dc:subject xml:lang="en">thrombosis</dc:subject>
	<dc:description xml:lang="en">Cardiovascular disease is the primary cause of mortality in the world due to the formation of blood clots or thrombi in blood vessels. Bacterial proteases commonly function as thrombus dissolver agents in the pharmaceutical industry. Bacterial isolate HSFI-10 (Holothuria scabra Fermented Intestine-10) previously isolated from Rusip fermented sea cucumber had demonstrated thrombolytic activity. This study aimed to produce crude protease of HSFI-10 strain at an optimized incubation time and determine the thrombolytic activity of crude and dialysate proteases on A, B, AB, and O blood types. Isolate HSFI-10 was first molecularly identified and found to be Bacillus cereus with a homology level of 99.80% with Bacillus cereus strain ST06. The optimum crude enzyme was obtained after 48-h incubation with an activity of 222.52 U/mL, which increased to 438.84 U/mL after ammonium sulfate precipitation and dialysis. Clot lysis activity of crude enzymes was measured based on the gravimetry method on blood in the ABO system, showing results that ranged from 68.99% to 69.76%, while the dialysate ranged from 81.16% to 82.52%. In conclusion, partial purification of bacterial protease could increase both its specific and thrombolytic activities on human blood in the ABO system, with only 1% activity variability between A, B, AB, and O blood types.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2023-08-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/1765</dc:identifier>
	<dc:identifier>10.11598/btb.2023.30.2.1765</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 30 No. 2 (2023): BIOTROPIA Vol. 30 No. 2 August 2023; 147 - 157</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2023.30.2.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/1765/746</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2023 Ainutajriani Ainutajriani, Sri Darmawati, Muhammad Ardi Afriansyah, Ragil Saptaningtyas, Stalis Norma Ethica, Dewi Seswita Zilda</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
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