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The POTENTIAL OF LACTIC ACID BACTERIA, ISOLATED FROM SEVERAL SOURCES, TO INHIBIT THE GROWTH OF Candida albicans ATCC10231

BIOTROPIA · Vol. 31 No. 2 (2024) · hlm. 146–156

DOI
10.11598/btb.2024.31.2.1868
ISSN
1907-770X, 0215-6334
Terbit
2024-08-07
Penerbit
SEAMEO BIOTROP
Bahasa
en
Lisensi
creativecommons.org/licenses/by-nc-nd/4.0

Abstrak

ARTICLE HIGHLIGHTS- Lactic acid bacteria have potential to control candidiasis or Candida albicans. - The novelty of this study is to advance the potential of lactic acid bacteria to control candidiasis infection in human, with a view to develop novel LAB-based probiotic candidates with capability to inhibit/prevent infection by C. albicans, the causative agent of candidiasis.ABSTRACTThe main aims of this research were to isolate and identify potential lactic acid bacteria (LAB) inhibitory to Candida albicans. The LAB sources were kimchi, honey and vaginal secrete of healthy women. They were isolated with a view to develop a novel alternative method with reduced use of antifungal agents in the treatment of patients infected by such fungal pathogen. Isolation of the LABs was conducted by applying dilution spread method on de Mann Rogosa Sharpe agar (MRSA) medium supplemented with bromo cresol purple (BCP) indicator. Once purified, they were tested for antagonism against C. albicans in dual culture assays. LAB isolates that showed significant inhibition against the pathogen were identified using 16s rDNA sequences and their sequences were aligned with those of known sequences deposited at the Gene Bank (http://www.ncbi.nlm.nih.gov). The results showed that 46 among more than 100 LABs isolated in this study significantly inhibited the growth of C. albicans in the in vitro dual culture assays, and all showed resistance property to antifungal agent (fluconazole). This indicated that they all have potential to be synergically applied with reduced use of fluconazole in the therapy. The most potential isolates (10 isolates) were closely related to three LAB species, namely Lactobacillus paracasei, Lacticaseibacillus paracasei, and Pediococcus pentosaceus, based on their 16s rDNA sequence similarities with those deposited in the GenBank.

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  • Udayana University
  • IPB University
  • Universitas Dhyana Pura
  • Ministry of Marine Affairs and Fisheries
  • North Dakota State University
  • Bandung Institute of Technology
  • Universitas PGRI Mahadewa Indonesia
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Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Putu Angga WiradanaUniversitas Dhyana Pura4
Kalidas ShettyNorth Dakota State University4
I Dewa Agung Panji DwipayanaUdayana University3
Anak Agung Ketut DarmadiUdayana University3
I Gede WidhiantaraUniversitas Dhyana Pura2
I Made Gde Sudyadnyana SandhikaUniversitas Dhyana Pura2
Anak Agung Ayu Putri PermatasariUniversitas Dhyana Pura2
Aris JatmikoMinistry of Marine Affairs and Fisheries2
I Wayan SuandaUniversitas PGRI Mahadewa Indonesia2
Ni Luh HaniUdayana University2
Martin A. LineUniversity of Tasmania2
I Gusti Ayu Agung SeptiariUdayana University2
G.K. GANDHIADIUdayana University1
Putu Veri SwastikaUdayana University1
Adi PancoroBandung Institute of Technology1
Ni Luh Putu AriwathiUdayana University1
Yaya RukayadiUniversiti Putra Malaysia1
Silvia Gabrina TonyesUdayana University1
Yenni CiawiUdayana University1
Ni Luh Putu Kayika FebryantiUdayana University1
Ni Putu Adriani AstitiUdayana University1
Eva HarlinaIPB University1
Bambang Pontjo PriosoeryantoIPB University1
Retno WulansariIPB University1
Nafrina LanniariIPB University1
Surachmi SetiyaningsihIPB University1
Rachmi RidhoGunadarma University1
Riski RostantinataIPB University1
Tamaki OkabayashiUniversity of Miyazaki1
Athalia RatnasariUdayana University1
Ni Wayan SudatriUdayana University1
Made NyandraUniversitas Dhyana Pura1
Adnorita Fandah OktarianiUdayana University1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">The POTENTIAL OF LACTIC ACID BACTERIA, ISOLATED FROM SEVERAL SOURCES, TO INHIBIT THE GROWTH OF Candida albicans ATCC10231</dc:title>
	<dc:creator xml:lang="en">Ramona, Yan</dc:creator>
	<dc:creator xml:lang="en">Darmayasa, Ida Bagus Gede</dc:creator>
	<dc:creator xml:lang="en">Dharmawan, Komang</dc:creator>
	<dc:creator xml:lang="en">Sudaryatma, Putu Eka</dc:creator>
	<dc:creator xml:lang="en">Teriyani, Ni Made</dc:creator>
	<dc:creator xml:lang="en">Batho, Yanuarius Felix</dc:creator>
	<dc:creator xml:lang="en">Inabuy, Fainmarinat Selviani</dc:creator>
	<dc:subject xml:lang="en">Candidiasis</dc:subject>
	<dc:subject xml:lang="en">Candida albicans</dc:subject>
	<dc:subject xml:lang="en">diversity</dc:subject>
	<dc:subject xml:lang="en">probiotic</dc:subject>
	<dc:subject xml:lang="en">vaginal candidiasis</dc:subject>
	<dc:subject xml:lang="en">Probiotics</dc:subject>
	<dc:subject xml:lang="en">Biocontrol</dc:subject>
	<dc:subject xml:lang="en">Microbiology</dc:subject>
	<dc:description xml:lang="en">ARTICLE HIGHLIGHTS- Lactic acid bacteria have potential to control candidiasis or Candida albicans. - The novelty of this study is to advance the potential of lactic acid bacteria to control candidiasis infection in human, with a view to develop novel LAB-based probiotic candidates with capability to inhibit/prevent infection by C. albicans, the causative agent of candidiasis.ABSTRACTThe main aims of this research were to isolate and identify potential lactic acid bacteria (LAB) inhibitory to Candida albicans. The LAB sources were kimchi, honey and vaginal secrete of healthy women. They were isolated with a view to develop a novel alternative method with reduced use of antifungal agents in the treatment of patients infected by such fungal pathogen. Isolation of the LABs was conducted by applying dilution spread method on de Mann Rogosa Sharpe agar (MRSA) medium supplemented with bromo cresol purple (BCP) indicator. Once purified, they were tested for antagonism against C. albicans in dual culture assays. LAB isolates that showed significant inhibition against the pathogen were identified using 16s rDNA sequences and their sequences were aligned with those of known sequences deposited at the Gene Bank (http://www.ncbi.nlm.nih.gov). The results showed that 46 among more than 100 LABs isolated in this study significantly inhibited the growth of C. albicans in the in vitro dual culture assays, and all showed resistance property to antifungal agent (fluconazole). This indicated that they all have potential to be synergically applied with reduced use of fluconazole in the therapy. The most potential isolates (10 isolates) were closely related to three LAB species, namely Lactobacillus paracasei, Lacticaseibacillus paracasei, and Pediococcus pentosaceus, based on their 16s rDNA sequence similarities with those deposited in the GenBank.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2024-08-07</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/lactic-acid-bacteria-antagonistic-against-candida-albicans</dc:identifier>
	<dc:identifier>10.11598/btb.2024.31.2.1868</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 31 No. 2 (2024): BIOTROPIA Vol. 31 No. 2 August 2024; 146 - 156</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2024.31.2.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/lactic-acid-bacteria-antagonistic-against-candida-albicans/791</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2024 Yan Ramona, Darmayasa, Komang, Putu Eka, Teriyani, Fainmarinat </dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
</oai_dc:dc>