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INDIGENOUS BACILLUS SPECIES ISOLATED FROM AEDES AEGYPTI LARVAE: ISOLATION, LARVICIDAL TOXICITY SCREENING, PHENOTYPIC CHARACTERIZATION, AND MOLECULAR IDENTIFICATION

BIOTROPIA · Vol. 30 No. 2 (2023) · hlm. 242–252

DOI
10.11598/btb.2023.30.2.1938
ISSN
1907-770X, 0215-6334
Terbit
2023-08-01
Penerbit
SEAMEO BIOTROP
Bahasa
en
Lisensi
creativecommons.org/licenses/by-nc-nd/4.0

Abstrak

Vector-borne diseases transmitted by mosquitoes are considered a significant public health problem worldwide. Aedes aegypti is one of the mosquito species responsible for transmitting these diseases. One environmentally friendly method of vector control is the use of microbial agents such as Bacillus species. This study aimed to explore investigate indigenous entomopathogenic bacteria of Bacillus species isolated from A. aegypti larvae. Larvae samples were collected from breeding sites of A. aegypti. All isolates underwent screening and affirmation confirmation tests to assess their larvicidal toxicity against A. aegypti larvae. Phenotypic characterizations and molecular identifications were conducted to determine the species of the Bacillus isolates based on similarity index and percent identity (%ID). Phylogenetic trees were used to compare the isolates with other Bacillus species. The results revealed 120 isolates of Bacillus species from A. aegypti larvae samples. Among them, three isolates (LS3.3, LS9.1, and LSD4.2) exhibited the highest larvicidal toxicity in the confirmation test, resulting in larval mortality rates of 100%, 96.7%, and 100%, respectively, after 48 hours of exposure. Molecular identifications, showed that LSD4.2 had a 99.16% ID with Bacillus velezensis, LS3.3 had a 98.22% ID with Bacillus mojavensis, and LS9.1 had a 99.93% ID with Bacillus subtilis. These three bacteria from the Bacillus genus have been reported to offer significant benefits to humans.

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  • Universitas Airlangga
  • Institut Teknologi Sepuluh Nopember
  • National Research and Innovation Agency
  • IPB University
  • Universitas Negeri Surabaya
  • Bandung Institute of Technology
  • Universitas Islam Negeri Sumatera Utara
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Rekan penulisOrganisasiArtikel bersama
Agus SupriyantoUniversitas Airlangga2
Triono Bagus SaputroInstitut Teknologi Sepuluh Nopember1
Hery PurnobasukiUniversitas Airlangga1
Dini ErmavitaliniInstitut Teknologi Sepuluh Nopember1
Ratna Syifa’a RahmahanaInstitut Teknologi Sepuluh Nopember1
Bilqis Naura Safira Rizam RizamBandung Institute of Technology1
IwanAfiliasi tidak diketahui1
Usman BetawiAfiliasi tidak diketahui1
Muhammad Iqbal Hanafi NasutionUniversitas Islam Negeri Sumatera Utara1
Dewi SukmaIPB University1
Sugiharto -Universitas Airlangga1
Yosephine Sri Wulan ManuharaUniversitas Airlangga1
Dwi WinarniUniversitas Airlangga1
Anjar Tri WibowoUniversitas Airlangga1
Ufairanisa IslamatasyaUniversitas Airlangga1
Idqa Nurtri BhaktiUniversitas Airlangga1
Boon Chin TanUniversity of Malaya1
SALAMUN SALAMUNUniversitas Airlangga1
Ni’matuzahroh Ni’matuzahrohUniversitas Airlangga1
Fatimah FatimahUniversitas Airlangga1
Tri NurhariyatiUniversitas Airlangga1
Izdihar TsanaUniversitas Airlangga1
Sri SumarsihUniversitas Airlangga1
Sofijan HadiUniversitas Airlangga1
Rizka Diah FitriUniversitas Airlangga1
Gilva IllaviUniversitas Airlangga1
RahayuAfiliasi tidak diketahui1
Yang Geun MoDankook University1
Eun Ji BaeAfiliasi tidak diketahui1
Apon Zaenal MustopaNational Research and Innovation Agency1
Tatenda Calvin ChihomboriIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">INDIGENOUS BACILLUS SPECIES ISOLATED FROM AEDES AEGYPTI LARVAE: ISOLATION, LARVICIDAL TOXICITY SCREENING, PHENOTYPIC CHARACTERIZATION, AND MOLECULAR IDENTIFICATION</dc:title>
	<dc:creator xml:lang="en">Salamun</dc:creator>
	<dc:creator xml:lang="en">Rizky Danang Susetyo</dc:creator>
	<dc:creator xml:lang="en">Hakimatul Husniyah</dc:creator>
	<dc:creator xml:lang="en">Almando Geraldi</dc:creator>
	<dc:creator xml:lang="en">Ni’matuzahroh</dc:creator>
	<dc:creator xml:lang="en">Fatimah</dc:creator>
	<dc:creator xml:lang="en">Farah Aisyah Nafidiastri</dc:creator>
	<dc:creator xml:lang="en">Nabilatun Nisa’</dc:creator>
	<dc:creator xml:lang="en">Salamy, Muhammad Fath Alhaqqi Sanis</dc:creator>
	<dc:subject xml:lang="en">Aedes aegypti</dc:subject>
	<dc:subject xml:lang="en">Bacillus mojavensis</dc:subject>
	<dc:subject xml:lang="en">Bacillus subtilis</dc:subject>
	<dc:subject xml:lang="en">Bacillus velezensis</dc:subject>
	<dc:subject xml:lang="en">Larvicidal Toxicity</dc:subject>
	<dc:description xml:lang="en">Vector-borne diseases transmitted by mosquitoes are considered a significant public health problem worldwide. Aedes aegypti is one of the mosquito species responsible for transmitting these diseases. One environmentally friendly method of vector control is the use of microbial agents such as Bacillus species. This study aimed to explore investigate indigenous entomopathogenic bacteria of Bacillus species isolated from A. aegypti larvae. Larvae samples were collected from breeding sites of A. aegypti. All isolates underwent screening and affirmation confirmation tests to assess their larvicidal toxicity against A. aegypti larvae. Phenotypic characterizations and molecular identifications were conducted to determine the species of the Bacillus isolates based on similarity index and percent identity (%ID). Phylogenetic trees were used to compare the isolates with other Bacillus species. The results revealed 120 isolates of Bacillus species from A. aegypti larvae samples. Among them, three isolates (LS3.3, LS9.1, and LSD4.2) exhibited the highest larvicidal toxicity in the confirmation test, resulting in larval mortality rates of 100%, 96.7%, and 100%, respectively, after 48 hours of exposure. Molecular identifications, showed that LSD4.2 had a 99.16% ID with Bacillus velezensis, LS3.3 had a 98.22% ID with Bacillus mojavensis, and LS9.1 had a 99.93% ID with Bacillus subtilis. These three bacteria from the Bacillus genus have been reported to offer significant benefits to humans.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2023-08-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/1938</dc:identifier>
	<dc:identifier>10.11598/btb.2023.30.2.1938</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 30 No. 2 (2023): BIOTROPIA Vol. 30 No. 2 August 2023; 242 - 252</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2023.30.2.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/1938/743</dc:relation>
	<dc:coverage xml:lang="en">Applied Microbiology</dc:coverage>
	<dc:rights xml:lang="en">Copyright (c) 2023 Salamun, Rizky Danang Susetyo, Hakimatul Husniyah, Almando Geraldi, Ni’matuzahroh, Fatimah, Farah Aisyah Nafidiastri, Nabilatun Nisa’</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
</oai_dc:dc>