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FURTHER STUDY ON TWO SPECIES OF LOACH FISHES (Cypriniformes: Nemacheilidae: Nemacheilus) BASED ON MORPHOLOGY AND MOLECULAR DATA

BIOTROPIA · Vol. 30 No. 3 (2023) · hlm. 329–345

DOI
10.11598/btb.2023.30.2.1942
ISSN
1907-770X, 0215-6334
Terbit
2023-12-07
Penerbit
SEAMEO BIOTROP
Bahasa
en
Lisensi
creativecommons.org/licenses/by-nc-nd/4.0

Abstrak

The identities of two local loaches, Nemacheilus chrysolaimos (Valenciennes, 1846) and N. fasciatus (Valenciennes, 1846) from six rivers, were obtained through a comprehensive examination of their morphology and molecular characteristics in Biltar Regency, East Java, Indonesia. Therefore, this study identified Nemacheilus spp. from Blitar based on morphology and partial sequence of COI. The meristic data obtained for N. chrysolaimos included DII. 7–8 (dorsal fin), AI. 3–5 (anal fin), PI. 9 (pectoral fin), VI. 6–7 (ventral fin), and C. 17 (caudal fin). On the other hand, N. fasciatus exhibited the following meristic data, namely D II 7–8 (dorsal fin), AI. 6 (anal fin), PI. 9–10 (pectoral fin), VI. 6–7 (ventral fin), and C. 17 (caudal fin). A significant difference was observed in the morphometric characteristics of N. fasciatus across various sampling sites, as determined by the Kruskal-Wallis Test. Furthermore, the nucleotide base composition sequences of Nemacheilus spp. consisted of Thiamine (T), Cytosine (C), Adenine (A), and Guanine (G) with a mean of 29.565%, 32.023%, 23.88%, and 16.244%. Maximum Likelihood (ML) and Minimum Evolution (ME) phylogenetic analysis was also conducted using the Kimura 2 Parameter model to establish two major clades on Nemacheilus spp. and one out-group significantly different from the Nemacheilus spp. The results showed that these major clades exhibited a close relationship at 100% bootstrap support and were grouped under the genus Nemacheilus. The study on Nemacheilus spp. from the Blitar locality differentiated COI sequences between N. fasciatus and N. chrysolaimos. Additionally, N. chrysolaimos, as inferred from reference sequences, was identified as the ancestral species to N. chrysolaimos MZB 26540 and MZB 26539. ABGD analyses, employing a prior maximal distance of 0.025, also indicated the separation of these species into distinct partitions. The integration of morphology and genetic data for Nemacheilus spp. should provide valuable insights for future genetic population studies and conservation initiatives.

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  • Universitas Negeri Surabaya
  • State University of Semarang
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  • National Research and Innovation Agency
  • Universitas Nahdlatul Ulama Pasuruan
  • Udayana University
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Yuli UtantoAfiliasi tidak diketahui5
Rusdarti RusdartiAfiliasi tidak diketahui3
Farid AhmadiAfiliasi tidak diketahui3
Hari WibawantoState University of Semarang3
Totok Sumaryanto FlorentinusState University of Semarang3
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Budiyono BudiyonoAfiliasi tidak diketahui2
Joko WidodoState University of Semarang2
Masrukhi MasrukhiAfiliasi tidak diketahui2
Tri Joko RaharjoAfiliasi tidak diketahui2
Samsudi SamsudiAfiliasi tidak diketahui2
Muhammad KhumaediAfiliasi tidak diketahui2
Sri WardaniUdayana University2
Firas KhaleylaUniversitas Negeri Surabaya2
Rusdianto RusdiantoNational Research and Innovation Agency2
Viona Angelina Erlan PanjaitanUniversitas Negeri Surabaya2
Adam Dwi RanggaUniversitas Negeri Surabaya2
Cahya Ajeng Valenta Tresna SulungUniversitas Negeri Surabaya2
Noorhidayah Binti MamatUniversity of Malaya2
Endhang SiswatiUniversitas Bhayangkara Surabaya1
Indah EpriliatiUniversitas Katolik Widya Mandala Surabaya1
Mochammad MuchidUniversitas Wijaya Putra1
Indra Pratama Putra SalmonIndonesia Open University1
Nurlinah NurlinahSunan Gunung Djati State Islamic University Bandung1
Andi Samsu AlamAfiliasi tidak diketahui1
Valen Octavia PakpahanAfiliasi tidak diketahui1
Hardono HardonoNational Research and Innovation Agency1
Isti HidayahState University of Semarang1
Maskur MaskurUniversitas Islam Negeri Ar-Raniry1
Yulianti YuliantiBandung Institute of Technology1
Haning FaridaAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">FURTHER STUDY ON TWO SPECIES OF LOACH FISHES (Cypriniformes: Nemacheilidae: Nemacheilus) BASED ON MORPHOLOGY AND MOLECULAR DATA</dc:title>
	<dc:creator xml:lang="en">Rahayu, Dwi Anggorowati</dc:creator>
	<dc:creator xml:lang="en">Kuntjoro, Sunu</dc:creator>
	<dc:creator xml:lang="en">Budijastuti, Widowati</dc:creator>
	<dc:creator xml:lang="en">Winarsih, Winarsih</dc:creator>
	<dc:creator xml:lang="en">Ambarwati, Reni</dc:creator>
	<dc:creator xml:lang="en">Nugroho, Endik Deni</dc:creator>
	<dc:creator xml:lang="en">Basith, Abdul</dc:creator>
	<dc:creator xml:lang="en">Kurniawan, Nia</dc:creator>
	<dc:creator xml:lang="en">Haryono, Haryono</dc:creator>
	<dc:subject xml:lang="en">DNA barcoding</dc:subject>
	<dc:subject xml:lang="en">morphology</dc:subject>
	<dc:subject xml:lang="en">Nemacheilus</dc:subject>
	<dc:subject xml:lang="en">phylogenetic</dc:subject>
	<dc:subject xml:lang="en">taxonomy </dc:subject>
	<dc:description xml:lang="en">The identities of two local loaches, Nemacheilus chrysolaimos (Valenciennes, 1846) and N. fasciatus (Valenciennes, 1846) from six rivers, were obtained through a comprehensive examination of their morphology and molecular characteristics in Biltar Regency, East Java, Indonesia. Therefore, this study identified Nemacheilus spp. from Blitar based on morphology and partial sequence of COI. The meristic data obtained for N. chrysolaimos included DII. 7–8 (dorsal fin), AI. 3–5 (anal fin), PI. 9 (pectoral fin), VI. 6–7 (ventral fin), and C. 17 (caudal fin). On the other hand, N. fasciatus exhibited the following meristic data, namely D II 7–8 (dorsal fin), AI. 6 (anal fin), PI. 9–10 (pectoral fin), VI. 6–7 (ventral fin), and C. 17 (caudal fin). A significant difference was observed in the morphometric characteristics of N. fasciatus across various sampling sites, as determined by the Kruskal-Wallis Test. Furthermore, the nucleotide base composition sequences of Nemacheilus spp. consisted of Thiamine (T), Cytosine (C), Adenine (A), and Guanine (G) with a mean of 29.565%, 32.023%, 23.88%, and 16.244%. Maximum Likelihood (ML) and Minimum Evolution (ME) phylogenetic analysis was also conducted using the Kimura 2 Parameter model to establish two major clades on Nemacheilus spp. and one out-group significantly different from the Nemacheilus spp. The results showed that these major clades exhibited a close relationship at 100% bootstrap support and were grouped under the genus Nemacheilus. The study on Nemacheilus spp. from the Blitar locality differentiated COI sequences between N. fasciatus and N. chrysolaimos. Additionally, N. chrysolaimos, as inferred from reference sequences, was identified as the ancestral species to N. chrysolaimos MZB 26540 and MZB 26539. ABGD analyses, employing a prior maximal distance of 0.025, also indicated the separation of these species into distinct partitions. The integration of morphology and genetic data for Nemacheilus spp. should provide valuable insights for future genetic population studies and conservation initiatives.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2023-12-07</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/1942</dc:identifier>
	<dc:identifier>10.11598/btb.2023.30.2.1942</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 30 No. 3 (2023): BIOTROPIA Vol. 30 No. 3 December 2023; 329 - 345</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2023.30.3.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/1942/738</dc:relation>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/1942/769</dc:relation>
	<dc:relation>10.11598/btb.2023.30.3.738</dc:relation>
	<dc:relation>10.11598/btb.2023.30.3.769</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2023 Dwi Rahayu</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
</oai_dc:dc>