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OPTIMIZATION OF KINETIN CONCENTRATIONS AND MEDIUM COMPOSITIONS FOR CITRUS SHOOT MULTIPLICATION FROM COTILEDONARY NODE

BIOTROPIA · Vol. 31 No. 1 (2024)

DOI
10.11598/btb.2024.31.1.2136
ISSN
1907-770X, 0215-6334
Terbit
2024-04-18
Penerbit
SEAMEO BIOTROP
Bahasa
en
Lisensi
creativecommons.org/licenses/by-nc-nd/4.0

Abstrak

Plant regeneration post-genetic transformation play an important an role in genome editing activities that should be optimized via several factors such as the composition of the medium and the concentration of plant growth regulators. This study aimed to optimize the kinetin concentrations and medium compositions for shoot multiplication originating from cotyledon node explants of several local citrus cultivars. The cotyledonary nodes from three citrus cultivars (Batu 55, Siam Madu, and Proksi-1 Agrihorti) were incubated in MS medium with Morel and Wetmore vitamins (VMW) supplemented with several kinetin concentrations (0; 0.2; 0.4; 0.6; 0.8; and 1 mg/L). The best kinetin concentrations for number of shoots variable were then combined with MT medium. The results showed that kinetin concentration at 0.8 mg/L gave the best number of shoot in Batu 55 cultivar as well as 1 mg/L concentration in Siam Madu and Proksi-1 Agrihorti cultivars. The combination 1 mg/L kinetin with Murashige and Tucker (MT) medium showed the best number of shoots, percentage of shoot formation, number of leaves, number of nodes, and shoot length in this study. This medium composition could be further used for shoot multiplication in genetic transformation in those three citrus cultivars, including genome editing activities in development of new improved citrus varieties.

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  • National Research and Innovation Agency
  • IPB University
  • Indonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development
  • Lampung University
  • University of Brawijaya
  • Universiti Malaysia Terengganu
  • Indonesian Institute of Sciences
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Rekan penulisOrganisasiArtikel bersama
Dyah Retno WulandariNational Research and Innovation Agency5
Sudarsono SudarsonoIPB University3
Dumaris Priskila PurbaIPB University3
Alina AkhidayaIndonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development3
Fajriyatus ShoidahAfiliasi tidak diketahui3
Kurniawan BudiartoNational Research and Innovation Agency3
Hadi Mokhamad YusufNational Research and Innovation Agency3
Muhammad Asya FadillahUniversity of Brawijaya3
Ali HusniNational Research and Innovation Agency3
Budi WaluyoUniversity of Brawijaya3
Puji LestariIndonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development2
Slamet Arif SusantoIPB University2
Tri Muji ErmayantiNational Research and Innovation Agency2
Muhammad Dima Iqbal HamdaniLampung University1
Muhammad Tino FajarLampung University1
Sulastri SulastriLampung University1
Nur AjijahAfiliasi tidak diketahui1
Rr. Sri HartatiAfiliasi tidak diketahui1
Rubiyo RubiyoAfiliasi tidak diketahui1
Erick RaynaltaIPB University1
Juanita ElinaIPB University1
Ika RoostikaNational Research and Innovation Agency1
HS EdisonAfiliasi tidak diketahui1
Habib RijzaaniIndonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development1
Mastur MasturIndonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development1
Rudiyanto RudiyantoUniversiti Malaysia Terengganu1
Darda EfendiIPB University1
Andri Fadillah MartinIndonesian Institute of Sciences1
Irdika MansurIPB University1
Endang SuhendangIPB University1
Julius Dwi NugrohoIPB University1
FatimahNational Research and Innovation Agency1
Arrin RosmalaCoca Cola (United States)1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">OPTIMIZATION OF KINETIN CONCENTRATIONS AND MEDIUM COMPOSITIONS FOR CITRUS SHOOT MULTIPLICATION FROM COTILEDONARY NODE </dc:title>
	<dc:creator xml:lang="en">Nugroho, Kristianto</dc:creator>
	<dc:creator xml:lang="en">Kosmiatin, Mia</dc:creator>
	<dc:creator xml:lang="en">Santoso, Tri Joko</dc:creator>
	<dc:creator xml:lang="en">Sukma, Dewi</dc:creator>
	<dc:creator xml:lang="en">Purwito, Agus</dc:creator>
	<dc:creator xml:lang="en">Husni, Ali</dc:creator>
	<dc:creator xml:lang="en">Martasari, Chaireni</dc:creator>
	<dc:subject xml:lang="en">cotyledonary node</dc:subject>
	<dc:subject xml:lang="en">genetic transformation</dc:subject>
	<dc:subject xml:lang="en">kinetin</dc:subject>
	<dc:subject xml:lang="en">Murashige and Tucker medium</dc:subject>
	<dc:subject xml:lang="en">shoot multiplication</dc:subject>
	<dc:subject xml:lang="en">plant tissue culture</dc:subject>
	<dc:subject xml:lang="en">biotechnology</dc:subject>
	<dc:description xml:lang="en">Plant regeneration post-genetic transformation play an important an role in genome editing activities that should be optimized via several factors such as the composition of the medium and the concentration of plant growth regulators. This study aimed to optimize the kinetin concentrations and medium compositions for shoot multiplication originating from cotyledon node explants of several local citrus cultivars. The cotyledonary nodes from three citrus cultivars (Batu 55, Siam Madu, and Proksi-1 Agrihorti) were incubated in MS medium with Morel and Wetmore vitamins (VMW) supplemented with several kinetin concentrations (0; 0.2; 0.4; 0.6; 0.8; and 1 mg/L). The best kinetin concentrations for number of shoots variable were then combined with MT medium. The results showed that kinetin concentration at 0.8 mg/L gave the best number of shoot in Batu 55 cultivar as well as 1 mg/L concentration in Siam Madu and Proksi-1 Agrihorti cultivars. The combination 1 mg/L kinetin with Murashige and Tucker (MT) medium showed the best number of shoots, percentage of shoot formation, number of leaves, number of nodes, and shoot length in this study. This medium composition could be further used for shoot multiplication in genetic transformation in those three citrus cultivars, including genome editing activities in development of new improved citrus varieties.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2024-04-18</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/2136</dc:identifier>
	<dc:identifier>10.11598/btb.2024.31.1.2136</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 31 No. 1 (2024): BIOTROPIA Vol. 31 No. 1 April 2024</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2024.31.1.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/2136/786</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2024 Kristianto Nugroho, Dr. Mia Kosmiatin, Dr. Tri Joko Santoso, Prof. Dewi Sukma, Prof. Agus Purwito, Dr. Ali Husni, Dr. Chaireni Martasari</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
</oai_dc:dc>