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INTRA-SPESIFIC DIVERSITY OF BUTTERFLY PEA (Clitoria ternatea L.) REVEALED BY ISSR WITH INVARIABLE ITS RECORDS

BIOTROPIA · Vol. 32 No. 1 (2025) · hlm. 57–68

DOI
10.11598/btb.2025.32.1.2362
ISSN
1907-770X, 0215-6334
Terbit
2025-04-28
Penerbit
SEAMEO BIOTROP
Bahasa
en
Lisensi
creativecommons.org/licenses/by-nc-nd/4.0

Abstrak

Article Highlights:- The ITS region exhibits no genetic variation among butterfly pea genotypes.- ISSR reveals high polymorphism, proving effective for genetic diversity analysis.- White single and purple single genotypes show the greatest genetic distance.- ISSR-6 ((CT)₈TG) demonstrates the highest polymorphism and reliability for assessment.- The ITS sequences have been deposited in GenBank under the accession IDs PQ198055–PQ198061. ABSTRACTClitoria ternatea L., a perennial plant in the Fabaceae, is recognized for its resilience in tropical climates and its diverse applications in both culinary and medicinal fields. However, the limited exploration of its genetic diversity constrains breeding efforts aimed at improving desirable traits. This limitation highlights the need to optimize selection strategies, identify superior genotypes, and preserve valuable genetic resources for long-term conservation and crop enhancement. This study aimed to explore genetic variation using molecular markers to analyze C. ternatea genotypes based on petal architecture and color differences. To assess the genetic diversity of C. ternatea, Sanger sequencing of the Internal Transcribed Spacer (ITS) region and Inter-Simple Sequence Repeat (ISSR) markers were applied to seven wild populations from Sleman, Yogyakarta. The ITS region exhibited no genetic variation, indicating its conserved nature and limited ability to differentiate genotypes. In contrast, ISSR markers effectively detected genetic variation, identifying 62 polymorphic fragments out of 162 total bands. The highest genetic distance (0.297) was observed between the WS and PS genotypes, whereas the double-petal genotypes (WD and PD) displayed the closest phenetic relationship. Among the ISSR primers, UBC-808, UBC-812, and ISSR-6 exhibited high PIC and RP values, confirming their reliability in genetic diversity analysis. These results underscore the utility of ISSR markers as a robust tool for genetic diversity assessment, offering valuable insights for breeding programs and germplasm conservation in C. ternatea.

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  • Universitas Gadjah Mada
  • National Research and Innovation Agency
  • Politeknik Bina Husada Kendari
  • Indonesian Institute of Sciences
  • Tokyo University of Agriculture
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  • Organisasi sama
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Rekan penulisOrganisasiArtikel bersama
Katon Waskito AjiUniversitas Gadjah Mada6
Rina Sri KasiamdariUniversitas Gadjah Mada6
Dwi Sendi PriyonoUniversitas Gadjah Mada4
Arif MuazamUniversitas Gadjah Mada3
Setyorini WidyayantiNational Research and Innovation Agency3
Eko Agus SuyonoUniversitas Gadjah Mada3
Rury EprilurahmanUniversitas Gadjah Mada3
Purnomo PurnomoUniversitas Gadjah Mada2
Purnomo PurnomoUniversitas Gadjah Mada2
Yudhistira NugrahaNational Research and Innovation Agency2
Kristamtini KristamtiniNational Research and Innovation Agency2
Zuliyati RohmahUniversitas Gadjah Mada2
Keiko T. NatsuakiTokyo University of Agriculture2
Endang SemiartiUniversitas Gadjah Mada2
Endah RetnaningrumUniversitas Gadjah Mada2
Fitri NadifahUniversitas Gadjah Mada2
Lukman HakimUniversitas Gadjah Mada2
Febrina Amaliya Rha’ifaUniversitas Gadjah Mada2
Amir HamidyIndonesian Institute of Sciences2
Rosichon UbaidillahIndonesian Institute of Sciences2
Diana FebriyantiUniversitas Gadjah Mada2
Muhammad IlyasNational Research and Innovation Agency1
Sri SusantiPoliteknik Bina Husada Kendari1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">INTRA-SPESIFIC DIVERSITY OF BUTTERFLY PEA (Clitoria ternatea L.) REVEALED BY ISSR WITH INVARIABLE ITS RECORDS</dc:title>
	<dc:creator xml:lang="en">Yusuf, Adib Fakhruddin</dc:creator>
	<dc:creator xml:lang="en">Latifah, Vida Rahma</dc:creator>
	<dc:creator xml:lang="en">Nurcahyati, Vivi Indah</dc:creator>
	<dc:creator xml:lang="en">Nurhasanah, Anggun Diyan</dc:creator>
	<dc:creator xml:lang="en">Widyasari, Adristi Shafa</dc:creator>
	<dc:creator xml:lang="en">Nainggolan, Ananto Puradi</dc:creator>
	<dc:creator xml:lang="en">Hasibuan, Aldy Riau Wansyah</dc:creator>
	<dc:creator xml:lang="en">Hidayat, Madyan Akmal</dc:creator>
	<dc:creator xml:lang="en">Karmilah, Karmilah</dc:creator>
	<dc:creator xml:lang="en">Pratiwi, Arini Dian</dc:creator>
	<dc:creator xml:lang="en">Aurantika, Rindu</dc:creator>
	<dc:creator xml:lang="en">Hasanah, Muslifah</dc:creator>
	<dc:creator xml:lang="en">Aristya, Ganies Riza</dc:creator>
	<dc:creator xml:lang="en">Handayani, Niken Satuti Nur</dc:creator>
	<dc:creator xml:lang="en">Arisuryanti, Tuty</dc:creator>
	<dc:creator xml:lang="en">Lesmana, Indra</dc:creator>
	<dc:creator xml:lang="en">Daryono, Budi Setiadi</dc:creator>
	<dc:subject xml:lang="en">butterfly pea</dc:subject>
	<dc:subject xml:lang="en">genetic distance</dc:subject>
	<dc:subject xml:lang="en">inter-simple sequence repeat</dc:subject>
	<dc:subject xml:lang="en">internal transcribed spacer</dc:subject>
	<dc:subject xml:lang="en">morphological variation</dc:subject>
	<dc:subject xml:lang="en">Plant Science</dc:subject>
	<dc:subject xml:lang="en">Molecular genetics</dc:subject>
	<dc:subject xml:lang="en">Plant Diversity</dc:subject>
	<dc:description xml:lang="en">Article Highlights:- The ITS region exhibits no genetic variation among butterfly pea genotypes.- ISSR reveals high polymorphism, proving effective for genetic diversity analysis.- White single and purple single genotypes show the greatest genetic distance.- ISSR-6 ((CT)₈TG) demonstrates the highest polymorphism and reliability for assessment.- The ITS sequences have been deposited in GenBank under the accession IDs PQ198055–PQ198061.
ABSTRACTClitoria ternatea L., a perennial plant in the Fabaceae, is recognized for its resilience in tropical climates and its diverse applications in both culinary and medicinal fields. However, the limited exploration of its genetic diversity constrains breeding efforts aimed at improving desirable traits. This limitation highlights the need to optimize selection strategies, identify superior genotypes, and preserve valuable genetic resources for long-term conservation and crop enhancement. This study aimed to explore genetic variation using molecular markers to analyze C. ternatea genotypes based on petal architecture and color differences. To assess the genetic diversity of C. ternatea, Sanger sequencing of the Internal Transcribed Spacer (ITS) region and Inter-Simple Sequence Repeat (ISSR) markers were applied to seven wild populations from Sleman, Yogyakarta. The ITS region exhibited no genetic variation, indicating its conserved nature and limited ability to differentiate genotypes. In contrast, ISSR markers effectively detected genetic variation, identifying 62 polymorphic fragments out of 162 total bands. The highest genetic distance (0.297) was observed between the WS and PS genotypes, whereas the double-petal genotypes (WD and PD) displayed the closest phenetic relationship. Among the ISSR primers, UBC-808, UBC-812, and ISSR-6 exhibited high PIC and RP values, confirming their reliability in genetic diversity analysis. These results underscore the utility of ISSR markers as a robust tool for genetic diversity assessment, offering valuable insights for breeding programs and germplasm conservation in C. ternatea.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2025-04-28</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:format>text/xml</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/2362</dc:identifier>
	<dc:identifier>10.11598/btb.2025.32.1.2362</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 32 No. 1 (2025): BIOTROPIA Vol. 32 No. 1 April 2025; 57 - 68</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2025.32.1.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/2362/840</dc:relation>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/2362/849</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Adib Fakhruddin Yusuf, Vida Rahma Latifah, Vivi Indah Nurcahyati, Anggun Diyan Nurhasanah, Adristi Shafa Widyasari, Ananto Puradi Nainggolan, Aldy Riau Wansyah Hasibuan, Madyan Akmal Hidayat, Karmilah, Arini Dian Pratiwi, Rindu Aurantika, Muslifah Hasanah, Ganies Riza Aristya, Niken Satuti Nur Handayani, Tuty Arisuryanti, Indra Lesmana, Budi Setiadi Daryono</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
</oai_dc:dc>