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MOLECULAR ANALYSIS OF WAXY GENE MARKERS IN SORGHUM CROSSES KD4 AND BONTEB GUNUNGKIDUL

BIOTROPIA · Vol. 32 No. 3 (2025) · hlm. 328–338

DOI
10.11598/btb.2025.32.3.2457
ISSN
1907-770X, 0215-6334
Terbit
2025-12-15
Penerbit
SEAMEO BIOTROP
Bahasa
en
Lisensi
creativecommons.org/licenses/by-nc-nd/4.0

Abstrak

HIGHLIGHTS- Local sorghum crosses show unique fixation of the waxy starch gene allele- Only Wxc allele is expressed, while other waxy alleles are not detected- Waxy allele expression strongly relates to low amylose grain quality- Marker-based selection supports breeding of soft-textured sorghum- Findings enhance sorghum use for food, feed, and industrial applicationsABSTRACTSorghum (Sorghum bicolor L.) is a food crop exhibiting resilience to extreme environmental conditions and potential to develop as an alternative food source. Quality of sorghum seeds is significantly influenced by starch composition in endosperm regulated by waxy (Wx) gene. This gene has several major alleles: Wxa, Wxb, and Wxc, playing roles in amylopectin and amylose syntheses. The purpose of study is to analyze the expression of Wx alleles in the crossbreeding of sorghum cultivars KD4 and Bonteb Gunungkidul. Main method used was molecular marker-based PCR method. Leaf and seed samples of sorghum were collected and extracted using the CTAB method to obtain high-quality genomic DNA. PCR reactions were performed with specific primers for each allele, and the amplification results were analyzed through 1.5% agarose gel electrophoresis. Statistical analysis was performed to ensure result significance through: Chi-Square Test to determine relationships between waxy allele expression with genetic segregation within cross populations, Allele Frequency Analysis to determine distribution of waxy genotypes within populations by comparing counts showing expressions of Wxa, Wxb, and Wxc, and Pearson Correlation Test to evaluate relationships between waxy gene expression with specific agronomic traits (e.g., amylose content). Main findings showed that only Wxc allele did exhibit a clear amplification band, while Wxa and Wxb did not show significant expression. Further studies are needed to understand the regulation of Wx gene expression and its potential implications to molecular selection, ultimately enhancing sorghum quality for both food and industrial applications.

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  • Universitas Gadjah Mada
  • National Research and Innovation Agency
  • IPB University
  • Sriwijaya University
  • Tokyo University of Agriculture
  • Universitas Merangin
  • Universitas Halu Oleo
  • Organisasi lain
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Rekan penulisOrganisasiArtikel bersama
Laila HanumSriwijaya University3
Eko Agus SuyonoUniversitas Gadjah Mada3
Ganies Riza AristyaUniversitas Gadjah Mada3
Purnomo PurnomoUniversitas Gadjah Mada3
Achmad GunawanNational Research and Innovation Agency3
Ani MugiasihNational Research and Innovation Agency3
Purnomo PurnomoUniversitas Gadjah Mada2
Adib Fakhruddin YusufUniversitas Gadjah Mada2
Purnomo PurnomoUniversitas Gadjah Mada2
Tuty ArisuryantiUniversitas Gadjah Mada2
Keiko T. NatsuakiTokyo University of Agriculture2
Endang SemiartiUniversitas Gadjah Mada2
Endah RetnaningrumUniversitas Gadjah Mada2
Sukmawan F. SantosaUniversitas Gadjah Mada2
R. RugayahUniversitas Gadjah Mada2
Fitri NadifahUniversitas Gadjah Mada2
SutardiUniversitas Halu Oleo2
Heni Sulistyawati Purwaning RahayuAfiliasi tidak diketahui2
KristamtiniAfiliasi tidak diketahui2
Pandu LaksonoNational Research and Innovation Agency2
Heni PurwaningsihNational Research and Innovation Agency2
Dewi SaharaNational Research and Innovation Agency2
Sugeng WidodoNational Research and Innovation Agency2
Diah Arina FahmiNational Research and Innovation Agency2
Joko TriastonoNational Research and Innovation Agency2
Arlyna Budi PustikaNational Research and Innovation Agency2
Niken Satuti Nur HandayaniUniversitas Gadjah Mada2
Yekti Asih PurwestriUniversitas Gadjah Mada2
Juanita HibatullahUniversitas Merangin1
Laila Uswatun ChasanahUniversitas Gadjah Mada1
Munif GhulamahdiIPB University1
Hajrial AswidinnoorIPB University1
Sintho Wahyuning ArdieIPB University1
Suwarno SuwarnoUniversitas Airlangga1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">MOLECULAR ANALYSIS OF WAXY GENE MARKERS IN SORGHUM CROSSES KD4 AND BONTEB GUNUNGKIDUL</dc:title>
	<dc:creator xml:lang="en">Muazam, Arif</dc:creator>
	<dc:creator xml:lang="en">Kristamtini, Kristamtini</dc:creator>
	<dc:creator xml:lang="en">Widyayanti, Setyorini</dc:creator>
	<dc:creator xml:lang="en">Nugraha, Yudhistira</dc:creator>
	<dc:creator xml:lang="en">Sri Kasiamdari, Rina</dc:creator>
	<dc:creator xml:lang="en">Budi Setiadi Daryono</dc:creator>
	<dc:subject xml:lang="en">genetics</dc:subject>
	<dc:subject xml:lang="en">local</dc:subject>
	<dc:subject xml:lang="en">plant breeding</dc:subject>
	<dc:subject xml:lang="en">sorghum</dc:subject>
	<dc:subject xml:lang="en">waxy gene</dc:subject>
	<dc:subject xml:lang="en">genetics, local, plant breeding, sorghum, waxy gene</dc:subject>
	<dc:subject xml:lang="en">Interpretation of Waxy Gene Allele Electrophoresis Results</dc:subject>
	<dc:subject xml:lang="en">Molecular Data Results on Sorghum Agronomics</dc:subject>
	<dc:subject xml:lang="en">Allele Frequency Analysis of Waxy Genotypes in Sorghum Populations</dc:subject>
	<dc:description xml:lang="en">HIGHLIGHTS- Local sorghum crosses show unique fixation of the waxy starch gene allele- Only Wxc allele is expressed, while other waxy alleles are not detected- Waxy allele expression strongly relates to low amylose grain quality- Marker-based selection supports breeding of soft-textured sorghum- Findings enhance sorghum use for food, feed, and industrial applicationsABSTRACTSorghum (Sorghum bicolor L.) is a food crop exhibiting resilience to extreme environmental conditions and potential to develop as an alternative food source. Quality of sorghum seeds is significantly influenced by starch composition in endosperm regulated by waxy (Wx) gene. This gene has several major alleles: Wxa, Wxb, and Wxc, playing roles in amylopectin and amylose syntheses. The purpose of study is to analyze the expression of Wx alleles in the crossbreeding of sorghum cultivars KD4 and Bonteb Gunungkidul. Main method used was molecular marker-based PCR method. Leaf and seed samples of sorghum were collected and extracted using the CTAB method to obtain high-quality genomic DNA. PCR reactions were performed with specific primers for each allele, and the amplification results were analyzed through 1.5% agarose gel electrophoresis. Statistical analysis was performed to ensure result significance through: Chi-Square Test to determine relationships between waxy allele expression with genetic segregation within cross populations, Allele Frequency Analysis to determine distribution of waxy genotypes within populations by comparing counts showing expressions of Wxa, Wxb, and Wxc, and Pearson Correlation Test to evaluate relationships between waxy gene expression with specific agronomic traits (e.g., amylose content). Main findings showed that only Wxc allele did exhibit a clear amplification band, while Wxa and Wxb did not show significant expression. Further studies are needed to understand the regulation of Wx gene expression and its potential implications to molecular selection, ultimately enhancing sorghum quality for both food and industrial applications.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2025-12-15</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/molecular-genetics-of-sorghum-crosses-kd4-and-bonteb-gunungkidul</dc:identifier>
	<dc:identifier>10.11598/btb.2025.32.3.2457</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 32 No. 3 (2025): BIOTROPIA Vol. 32 No. 3 December 2025; 328 - 338</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2025.32.3.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/molecular-genetics-of-sorghum-crosses-kd4-and-bonteb-gunungkidul/924</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 arif muazam, Kristamtini, Setyorini Widyayanti, Yudhistira Nugraha, Rina Sri Kasiamdari, Budi Setiadi Daryono</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
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