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Enzymatic Transesterification Using Different Immobilized Lipases and its Biodiesel Effect on Gas Emission

Bulletin of Chemical Reaction Engineering & Catalysis · Vol. ume (2024) · hlm. 265–274

DOI
10.9767/bcrec.20143
ISSN
1978-2993
Terbit
2024-08-30
Penerbit
Masyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS)
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Biodiesel, a third-generation bio-fuels, offering several advantages over regular diesel fuel. Waste cooking oil (WCO) emerges as an ideal feedstock due to its availability and easy accessibility. In this work, biodiesel is utilized from two different types of immobilized lipases: Rhizomucor miehei lipase (RMIM) and Candida antarctica lipase B (CALB). The impact of the molar ratio of oil to methyl acetate (1:3-1:12) was evaluated for both lipases, and the resultant biodiesel was tested in diesel engine. The enzymatic transesterification was carried out in ultrasonic assistance and the results showed that the greatest yield of 81.20% at 45℃, using CALB as a biocatalyst, 1.8% (w/v) lipase and oil to methyl acetate molar ratio of 1:12 within 3 hours. Triacetin, by-product was determined their concentration for each molar ratio and analyzed using FTIR range of 500cm-1 to 4000cm-1, revealing a significant absorption peak at 1238.90cm-1. Biodiesel was blended with commercial diesel fuel in varying quantities of 7, 10, and 20% by volume (B20). The results were compared to Industrial Diesel Fuel 7% (B7) and Commercial Diesel Fuel 10% (B10). NOx and CO2 emission drops as the percentage of diesel/biodiesel blends increases, supporting WCO as a cost-effective biodiesel feedstock with low petrol pollution.

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Wahid Akhsin Budi Nur SidiqState University of Semarang1
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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.bcrec.id/index.php/bcrec/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Enzymatic Transesterification Using Different Immobilized Lipases and its Biodiesel Effect on Gas Emission</dc:title>
	<dc:creator>Mohamad Nor, Nur Fatin Syafiqah</dc:creator>
	<dc:creator>Veny, Harumi</dc:creator>
	<dc:creator>Hamzah, Fazlena</dc:creator>
	<dc:creator>Muhd Rodhi, Miradatul Najwa</dc:creator>
	<dc:creator>Kusumaningtyas, Ratna Dewi</dc:creator>
	<dc:creator>Prasetiawan, Haniif</dc:creator>
	<dc:creator>Hartanto, Dhoni</dc:creator>
	<dc:creator>Sulaiman, Sarina</dc:creator>
	<dc:creator>Sazali, Rozana Azrina</dc:creator>
	<dc:subject xml:lang="en-US">Biodiesel; Waste Cooking Oil; Transesterification; Lipases; Biodiesel Blends</dc:subject>
	<dc:description xml:lang="en-US">Biodiesel, a third-generation bio-fuels, offering several advantages over regular diesel fuel. Waste cooking oil (WCO) emerges as an ideal feedstock due to its availability and easy accessibility. In this work, biodiesel is utilized from two different types of immobilized lipases: Rhizomucor miehei lipase (RMIM) and Candida antarctica lipase B (CALB). The impact of the molar ratio of oil to methyl acetate (1:3-1:12) was evaluated for both lipases, and the resultant biodiesel was tested in diesel engine. The enzymatic transesterification was carried out in ultrasonic assistance and the results showed that the greatest yield of 81.20% at 45℃, using CALB as a biocatalyst, 1.8% (w/v) lipase and oil to methyl acetate molar ratio of 1:12 within 3 hours. Triacetin, by-product was determined their concentration for each molar ratio and analyzed using FTIR range of 500cm-1 to 4000cm-1, revealing a significant absorption peak at 1238.90cm-1. Biodiesel was blended with commercial diesel fuel in varying quantities of 7, 10, and 20% by volume (B20). The results were compared to Industrial Diesel Fuel 7% (B7) and Commercial Diesel Fuel 10% (B10). NOx and CO2 emission drops as the percentage of diesel/biodiesel blends increases, supporting WCO as a cost-effective biodiesel feedstock with low petrol pollution.</dc:description>
	<dc:publisher xml:lang="en-US">Masyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS)</dc:publisher>
	<dc:date>2024-08-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.bcrec.id/index.php/bcrec/article/view/20143</dc:identifier>
	<dc:identifier>10.9767/bcrec.20143</dc:identifier>
	<dc:source xml:lang="en-US">Bulletin of Chemical Reaction Engineering &amp; Catalysis; 2024: BCREC Volume 19 Issue 2 Year 2024 (August 2024); 265-274</dc:source>
	<dc:source>1978-2993</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.bcrec.id/index.php/bcrec/article/view/20143/9702</dc:relation>
	<dc:relation>https://journal.bcrec.id/index.php/bcrec/article/downloadSuppFile/20143/5175</dc:relation>
	<dc:relation>https://journal.bcrec.id/index.php/bcrec/article/downloadSuppFile/20143/5176</dc:relation>
	<dc:relation>https://journal.bcrec.id/index.php/bcrec/article/downloadSuppFile/20143/5177</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2024 by Authors, Published by BCREC Publishing Group</dc:rights>
	<dc:rights xml:lang="en-US">https://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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