Akses terbuka Jurnal SINTA 1
STUDI KARAKTERISASI ANGGREK SECARA SITOLOGI DALAM RANGKA PELESTARIAN PLASMA NUTFAH
Caraka Tani: Journal of Sustainable Agriculture · Vol. 29 No. 1 (2017) · hlm. 25–30
- ISSN
- 2599-2570, 2613-9456
- Terbit
- 2017-08-22
- Penerbit
- Universitas Sebelas Maret
- Bahasa
- eng
Abstrak
Character was very important cytological nature orchids studied to support the success of breeding orchids. The purpose of the research to study the cytological characters orchid number, size) and chromosome karyotype in order to conserve germplasm. The study was conducted at the Laboratory of Biotechnology Faculty of Agriculture University Sebelas Maret Surakarta. Materials research Paphiophedilum glaucophylum, Coelogyne spesiosa, Dendrobium crumenantum, Dendrobium mutabile,, Bulbophyllum. Blumei dan Bulbophyllum beflorum. The method uses squashing methods include: fixation: Carnoy solution of 2 (6 ethanol: 3 chloroform: 1 glacial acetic acid 45 %), hydrolysis (1 N HCl) and staining chromosomes (aceto - orcein solution 2 %). Observations (number, size, shape) chromosomes with a light microscope. The results showed that the studied semuia orchids have the same chromosome number is 2n = 38, medium size chromosomes Paphiopedilum glaucophyllum 4.26 ± 0.14 to 9.73 ± 0.19, Coelogyne spesiosa 0.35 ± 0.03 to 6.30 ± 0.84, Dendrobium crumenantum 2.46 ± 0.20 until 5.33 ± 0.02 Dendrobium mutabile 2.56 ± 0.72 until 6.54 ± 0.73, Bulbophyllum blume 2.76 ± 0.09 until 6.07 ± 0.43 and Bulbophyllum beflorum 1.04 ± 0.07 sampai 1.35 ± 0.16. Karyotype patterns all orchids studied 2n = 38 m except Coelogyne spesiosa 2n = 37 m+ 1 ak and Dendrobium mutabile 2n = 37 m+ 1 sm.
Kata kunci
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- Sebelas Maret University
- National Research and Innovation Agency
- University of Indonesia
- Gifu University
- Afiliasi tidak diketahui
- Organisasi berbeda
- Organisasi sama
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Tampilkan sebagai tabel
| Rekan penulis | Organisasi | Artikel bersama |
|---|---|---|
| Slamet Minardi | Sebelas Maret University | 6 |
| Supriyadi Supriyadi | Sebelas Maret University | 4 |
| Jauhari Syamsiyah | Sebelas Maret University | 4 |
| Agus Budiyono | Sebelas Maret University | 4 |
| Sumani Sumani | Sebelas Maret University | 3 |
| Vita Ratri Cahyani | Sebelas Maret University | 3 |
| Retno Rosariastuti | Sebelas Maret University | 3 |
| Sudadi Sudadi | Sebelas Maret University | 3 |
| Purwanto Purwanto | Sebelas Maret University | 3 |
| Pardono Pardono | Sebelas Maret University | 3 |
| Sumijati Sumijati | Sebelas Maret University | 3 |
| Febriana Ida Dwi Ariyanti | Sebelas Maret University | 2 |
| Ganjar Herdiansyah | Sebelas Maret University | 2 |
| Widyatmani Sih Dewi | Sebelas Maret University | 2 |
| Nunik Puji Lestari | Sebelas Maret University | 2 |
| Dwi Priyo Ariyanto | Afiliasi tidak diketahui | 2 |
| Jihad Khadaffi | Sebelas Maret University | 1 |
| Nur Machfiroh | Sebelas Maret University | 1 |
| Okta Loveana | Sebelas Maret University | 1 |
| Rani Rahmawati | Sebelas Maret University | 1 |
| Srie Juli Rachmawatie | Sebelas Maret University | 1 |
| Henricus E. A. Hendrata | Sebelas Maret University | 1 |
| Sri Widadi | Sebelas Maret University | 1 |
| F C Mentari | Sebelas Maret University | 1 |
| Supriyono Supriyono | Sebelas Maret University | 1 |
| Desi Putri Hastuti | Sebelas Maret University | 1 |
| Sukaya Sukaya | Sebelas Maret University | 1 |
| Almar Aminudin | Sebelas Maret University | 1 |
| Rahayu Rahayu | Sebelas Maret University | 1 |
| Komariah Komariah | Sebelas Maret University | 1 |
| Setiawan Khoirul Himmi | National Research and Innovation Agency | 1 |
| Didi Tarmadi | National Research and Innovation Agency | 1 |
| Bramantyo Wikantyoso | National Research and Innovation Agency | 1 |
| Sri Rezeki Febriani | Sebelas Maret University | 1 |
| Muhamad Khoiru Zaki | Gifu University | 1 |
| Triyem Triyem | University of Indonesia | 1 |
| Herry Cahyana | University of Indonesia | 1 |
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