Regulation of Reactive Oxygen Species by Sodium Selenite in AndroMed® Extender Enhances Post-Thaw Saanen Buck Semen Quality

Erlindra, Caterina Palma; Diatmono, Dio Fico Felsidan; Padmawati, Fransisca Gani; Sastranegara, Arina Dinar Nurfadilla; Sitaresmi, Pradita Iustitia; Bintara, Sigit; Widayati, Diah Tri

doi:10.20961/carakatani.v41i1.108422

Akses terbuka Jurnal SINTA 1

Regulation of Reactive Oxygen Species by Sodium Selenite in AndroMed® Extender Enhances Post-Thaw Saanen Buck Semen Quality

Caraka Tani: Journal of Sustainable Agriculture · Vol. 41 No. 1 (2026) · hlm. 32–45

DOI: 10.20961/carakatani.v41i1.108422
ISSN: 2599-2570, 2613-9456
Terbit: 2026-01-05
Penerbit: Universitas Sebelas Maret
Bahasa: eng
Lisensi: creativecommons.org/licenses/by-nc/4.0

Abstrak

Semen cryopreservation is known to cause considerable damage to spermatozoa through the generation of free radicals. This damage can potentially be mitigated by the use of potent antioxidants, such as sodium selenite, which does not impair semen quality when administered in small doses. This study aimed to assess the impact of low-dose sodium selenite supplementation on post-thaw semen quality in Saanen bucks. Semen was collected from 2 superior Saanen bucks and evaluated according to standard criteria before dilution and freezing. Cryopreserved semen was stored in liquid nitrogen (LN2) at a temperature of -196 °C for 90 days. The experimental treatments involved the addition of sodium selenite to the AndroMed® diluent medium at 3 different concentrations: without supplementation/control (C), 5 ppm (T1), and 10 ppm (T2). Frozen semen was thawed at 37 °C in a water bath for 30 seconds before post-thaw quality assessment. The parameters assessed included motility, viability, abnormalities, plasma membrane integrity (PMI), acrosome membrane integrity (AMI), and reactive oxygen species (ROS). The data were statistically analyzed using One-Way analysis of variance (ANOVA). The results showed that the addition of sodium selenite had a significant effect (p 0.05). Interestingly, T1 resulted in the most substantial improvements, yielding the highest percentages of post-thaw motility (71.81±1.05%), viability (73.36±1.08%), PMI (77.49±1.68%), and AMI (79.29±0.63%), as well as the lowest ROS production (13.67±0.50%). In conclusion, the addition of 5 ppm sodium selenite enhances post-thaw semen quality in Saanen bucks.

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Universitas Gadjah Mada
National Research and Innovation Agency
Tanjungpura University
University of Brawijaya
Universitas Tidar
Nagoya University
IPB University
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Rekan penulis	Organisasi	Artikel bersama
Budi Prasetyo Widyobroto	Universitas Gadjah Mada	4
Sungkono Sungkono	Universitas Gadjah Mada	4
Andrian Syah	Universitas Gadjah Mada	4
Herdis Herdis	National Research and Innovation Agency	4
Satoshi Ohkura	Nagoya University	4
Kustantinah	Afiliasi tidak diketahui	2
Tety Hartatik	Universitas Gadjah Mada	2
Nur Hidayah	Universitas Tidar	1
Cuk Tri Noviandi	Universitas Gadjah Mada	1
Andriyani Astuti	Universitas Gadjah Mada	1
Kustantinah Kustantinah	Universitas Gadjah Mada	1
Muhammad Halim Natsir	University of Brawijaya	1
V.M. Ani Nurgiartiningsih	University of Brawijaya	1
Yuli Arif Tribudi	Tanjungpura University	1
Dwi Gusmalawati	Tanjungpura University	1
Maria Ulfah	IPB University	1
Aprilianna Putri Zahara Nafsina Luvita Sari	Padjadjaran University	1
Dwi Nur Happy Hariyono	Khairun University	1
Richard P. M. A. Crooijmans	Wageningen University & Research	1
Alek Ibrahim	National Research and Innovation Agency	1
Puji Astuti	Universitas Gadjah Mada	1
S. Sunendar	Afiliasi tidak diketahui	1
Kresno Suharto	Afiliasi tidak diketahui	1
Asmarani Kusumawati	Afiliasi tidak diketahui	1
Aris Junaidi	Afiliasi tidak diketahui	1
D. Puspitasari	Universitas Gadjah Mada	1
Fatimah Az Zahra Chairunissa	Universitas Gadjah Mada	1
F. J. Fadillah	Universitas Gadjah Mada	1
N. P. Ningrum	Universitas Gadjah Mada	1
Agung Irawan	Sebelas Maret University	1
A Astuti	Universitas Gadjah Mada	1

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Sumber metadata: OAI-PMH (metadata jurnal) http://jurnal.uns.ac.id/carakatani/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.

Metadata asli (oai_dc)

<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Regulation of Reactive Oxygen Species by Sodium Selenite in AndroMed® Extender Enhances Post-Thaw Saanen Buck Semen Quality</dc:title>
	<dc:creator>Erlindra, Caterina Palma</dc:creator>
	<dc:creator>Diatmono, Dio Fico Felsidan</dc:creator>
	<dc:creator>Padmawati, Fransisca Gani</dc:creator>
	<dc:creator>Sastranegara, Arina Dinar Nurfadilla</dc:creator>
	<dc:creator>Sitaresmi, Pradita Iustitia</dc:creator>
	<dc:creator>Bintara, Sigit</dc:creator>
	<dc:creator>Widayati, Diah Tri</dc:creator>
	<dc:subject xml:lang="en-US">agriculture conservation; animal welfare; biotechnology</dc:subject>
	<dc:subject xml:lang="en-US">frozen semen; ROS; Saanen buck; sodium selenite; sperm quality</dc:subject>
	<dc:description xml:lang="en-US">Semen cryopreservation is known to cause considerable damage to spermatozoa through the generation of free radicals. This damage can potentially be mitigated by the use of potent antioxidants, such as sodium selenite, which does not impair semen quality when administered in small doses. This study aimed to assess the impact of low-dose sodium selenite supplementation on post-thaw semen quality in Saanen bucks. Semen was collected from 2 superior Saanen bucks and evaluated according to standard criteria before dilution and freezing. Cryopreserved semen was stored in liquid nitrogen (LN2) at a temperature of -196 °C for 90 days. The experimental treatments involved the addition of sodium selenite to the AndroMed® diluent medium at 3 different concentrations: without supplementation/control (C), 5 ppm (T1), and 10 ppm (T2). Frozen semen was thawed at 37 °C in a water bath for 30 seconds before post-thaw quality assessment. The parameters assessed included motility, viability, abnormalities, plasma membrane integrity (PMI), acrosome membrane integrity (AMI), and reactive oxygen species (ROS). The data were statistically analyzed using One-Way analysis of variance (ANOVA). The results showed that the addition of sodium selenite had a significant effect (p &amp;lt; 0.05) on motility, viability, PMI, AMI, and ROS production, but did not affect the abnormality (p &amp;gt; 0.05). Interestingly, T1 resulted in the most substantial improvements, yielding the highest percentages of post-thaw motility (71.81±1.05%), viability (73.36±1.08%), PMI (77.49±1.68%), and AMI (79.29±0.63%), as well as the lowest ROS production (13.67±0.50%). In conclusion, the addition of 5 ppm sodium selenite enhances post-thaw semen quality in Saanen bucks.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Sebelas Maret</dc:publisher>
	<dc:contributor xml:lang="en-US">Universitas Gadjah Mada</dc:contributor>
	<dc:date>2026-01-05</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jurnal.uns.ac.id/carakatani/article/view/108422</dc:identifier>
	<dc:identifier>10.20961/carakatani.v41i1.108422</dc:identifier>
	<dc:source xml:lang="en-US">Caraka Tani: Journal of Sustainable Agriculture; Vol 41, No 1 (2026): January; 32-45</dc:source>
	<dc:source>2599-2570</dc:source>
	<dc:source>2613-9456</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jurnal.uns.ac.id/carakatani/article/view/108422/pdf</dc:relation>
	<dc:relation>https://jurnal.uns.ac.id/carakatani/article/downloadSuppFile/108422/23405</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2026 Caraka Tani: Journal of Sustainable Agriculture</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>