Melola Discovery

← Melola Discovery

Akses terbuka Jurnal SINTA 1

Fall Armyworm Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) in East Nusa Tenggara Province of Indonesia: Genetic Characterization and Strain Detection

Caraka Tani: Journal of Sustainable Agriculture · Vol. 39 No. 2 (2024) · hlm. 439–450

DOI
10.20961/carakatani.v39i2.91748
ISSN
2599-2570, 2613-9456
Terbit
2024-09-24
Penerbit
Universitas Sebelas Maret
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) is an invasive pest of corn plants spreading throughout the world, including East Nusa Tenggara (NTT), Indonesia. Despite the wide distribution, there is a lack of information on the strain or genetic diversity of the pest in NTT. Therefore, this study aimed to determine the strain of S. frugiperda from several areas in NTT with a molecular method using cytochrome oxidase subunit 1 (COI) and triose phosphate isomerase (Tpi) gene markers. The samples were collected from 3 islands: Timor, Flores, and Sumba. Amplification of the marker genes was carried out using 3 specific primers to identify the strain obtained from samples. Subsequently, polymerase chain reaction (PCR) products were sequenced and the DNA sequences were analyzed using the BioEdit and BLAST programs. Phylogeny analyses were carried out using the MEGA 11 program to verify the strain group of samples with reference isolates from other countries found in GenBank. The PCR results showed product amplicon size 811 bp for the CO1A marker. Based on phylogeny tree analysis using COI marker, S. frugiperda from NTT showed 2 clades, namely corn and rice strains. The characterization results showed that S. frugiperda in NTT comprised 63.6% corn and 36.4% rice strains. COIB classified S. frugiperda from NTT into the h4 haplotype subgroup, while Tpi gene marker was in the corn strain. This study provided valuable information regarding the strain of S. frugiperda in Indonesia to determine the appropriate control strategy.

Kata kunci

Jaringan kolaborasi para penulis

21 penulis · 49 tautan kepenulisan bersama · 20 lintas organisasi

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • Hasanuddin University
  • Universitas Ichsan Sidenreng Rappang
  • Universitas Flores
  • Institut Agama Islam Negeri Kendari
  • Al Asyariah Mandar University
  • National Research and Innovation Agency
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Tamrin AbdullahHasanuddin University2
Sylvia SjamHasanuddin University2
Sri Nur Aminah NgatiminHasanuddin University1
Ahdin GassaHasanuddin University1
Kartini KartiniInstitut Agama Islam Negeri Kendari1
Kaimuddin KaimuddinHasanuddin University1
Tutik KuswinantiHasanuddin University1
Hikmahwati HikmahwatiAl Asyariah Mandar University1
SATRIANI GASSINGHasanuddin University1
Amiruddin AminHasanuddin University1
Sulaeha ThamrinHasanuddin University1
Nurul Syafira ZulianaHasanuddin University1
Nurasiah DjaenuddinNational Research and Innovation Agency1
JumardiUniversitas Ichsan Sidenreng Rappang1
DesrianiUniversitas Ichsan Sidenreng Rappang1
Muh SandyUniversitas Ichsan Sidenreng Rappang1
Erwin ErwinHasanuddin University1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) http://jurnal.uns.ac.id/carakatani/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Fall Armyworm Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) in East Nusa Tenggara Province of Indonesia: Genetic Characterization and Strain Detection</dc:title>
	<dc:creator>Puu, Yustina Maria Silvia Wonga</dc:creator>
	<dc:creator>Daud, Itji Diana</dc:creator>
	<dc:creator>Nasruddin, Andi</dc:creator>
	<dc:creator>Melina, Melina</dc:creator>
	<dc:subject xml:lang="en-US">Pest Plant Sciences</dc:subject>
	<dc:subject xml:lang="en-US">COI; genetic diversity; NTT; Spodoptera frugiperda; Tpi</dc:subject>
	<dc:description xml:lang="en-US">Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) is an invasive pest of corn plants spreading throughout the world, including East Nusa Tenggara (NTT), Indonesia. Despite the wide distribution, there is a lack of information on the strain or genetic diversity of the pest in NTT. Therefore, this study aimed to determine the strain of S. frugiperda from several areas in NTT with a molecular method using cytochrome oxidase subunit 1 (COI) and triose phosphate isomerase (Tpi) gene markers. The samples were collected from 3 islands: Timor, Flores, and Sumba. Amplification of the marker genes was carried out using 3 specific primers to identify the strain obtained from samples. Subsequently, polymerase chain reaction (PCR) products were sequenced and the DNA sequences were analyzed using the BioEdit and BLAST programs. Phylogeny analyses were carried out using the MEGA 11 program to verify the strain group of samples with reference isolates from other countries found in GenBank. The PCR results showed product amplicon size 811 bp for the CO1A marker. Based on phylogeny tree analysis using COI marker, S. frugiperda from NTT showed 2 clades, namely corn and rice strains. The characterization results showed that S. frugiperda in NTT comprised 63.6% corn and 36.4% rice strains. COIB classified S. frugiperda from NTT into the h4 haplotype subgroup, while Tpi gene marker was in the corn strain. This study provided valuable information regarding the strain of S. frugiperda in Indonesia to determine the appropriate control strategy.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Sebelas Maret</dc:publisher>
	<dc:contributor xml:lang="en-US">Center for Education Financial Services (PUSLAPDIK) through the Indonesian Endowment Fund for Education (LPDP)</dc:contributor>
	<dc:date>2024-09-24</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jurnal.uns.ac.id/carakatani/article/view/91748</dc:identifier>
	<dc:identifier>10.20961/carakatani.v39i2.91748</dc:identifier>
	<dc:source xml:lang="en-US">Caraka Tani: Journal of Sustainable Agriculture; Vol 39, No 2 (2024): October; 439-450</dc:source>
	<dc:source>2599-2570</dc:source>
	<dc:source>2613-9456</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jurnal.uns.ac.id/carakatani/article/view/91748/pdf</dc:relation>
	<dc:relation>https://jurnal.uns.ac.id/carakatani/article/downloadSuppFile/91748/18361</dc:relation>
	<dc:relation>https://jurnal.uns.ac.id/carakatani/article/downloadSuppFile/91748/18841</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2024 Caraka Tani: Journal of Sustainable Agriculture</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>