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Cytotoxicity test of 40, 50 and 60% citric acid as dentin conditioner by using MTT assay on culture cell line

Dental Journal (Majalah Kedokteran Gigi) · Vol. 41 No. 3 (2008) · hlm. 103–106

DOI
10.20473/j.djmkg.v41.i3.p103-106
ISSN
2442-9740, 1978-3728
Terbit
2008-09-01
Penerbit
Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en
Bahasa
eng

Abstrak

Background: Open dentin is always covered by smear layer, therefore before restoration is performed, cavity or tooth which has been prepared should be clean from dirt. The researchers suggested that clean dentin surface would reach effective adhesion between resin and tooth structure, therefore dentin conditioner like citric acid was used to reach the condition. Even though citric acid is not strong acid but it can be very erosive to oral mucous. Several requirements should be fulfilled for dental product such as non toxic, non irritant, biocompatible and should not have negative effect against local, systemic or biological environment. Cytotoxicity test was apart of biomaterial evaluation and needed for standard screening. Purpose: This study was to know the cytotoxicity of 40, 50, 60% citric acid as dentin conditioner using MTT assay. Method: This study is an experimental research using the Post-Test Only Control Group Design. Six samples of each 40, 50 and 60% citric acid for citotoxicity test using MTT assay. The density of optic formazan indicated the number of living cells. All data were statistically analyzed by one way ANOVA. Result: The percentage of living cells in 40, 50 and 60% citric acid were 95.14%, 93.42% and 93.14%. Conclusion: Citric acid is non toxic and safe to be used as dentine conditioner.

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  • Universitas Airlangga
  • Universitas Hang Tuah
  • State University of Semarang
  • Lambung Mangkurat University
  • Universitas Gadjah Mada
  • Pelita Harapan University
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Rekan penulisOrganisasiArtikel bersama
Istiati IstiatiUniversitas Airlangga3
Ira ArundinaUniversitas Airlangga3
Ni Putu Mira SumartaUniversitas Airlangga2
Coen Pramono DanudiningratUniversitas Airlangga2
Nita NaomiUniversitas Airlangga2
Sisca Meida WatiUniversitas Airlangga1
Wisnu Setyari JUniversitas Airlangga1
Fitria RahmitasariUniversitas Hang Tuah1
Istiati SoehardjoUniversitas Airlangga1
Jenny SunarianiUniversitas Airlangga1
Agniz Nur AuliaUniversitas Airlangga1
Yuliati YuliatiState University of Semarang1
Dian Widya DamaiyantiUniversitas Hang Tuah1
Dania MaharaniUniversitas Airlangga1
Gunawan OentaryoLambung Mangkurat University1
R. Aries MuharramUniversitas Airlangga1
Wisnu Setyari JuliastutiUniversitas Airlangga1
Karina Awanis AdlaUniversitas Airlangga1
Retno Pudji RahayuUniversitas Airlangga1
Muhammad LuthfiUniversitas Airlangga1
Aqsa Sjuhada OkiUniversitas Airlangga1
Moch. Febi AlviansyahUniversitas Airlangga1
Rafael GerrardUniversitas Airlangga1
Yoakim Leok TaboyAfiliasi tidak diketahui1
Agatha Sherly Ivana DewiUniversitas Gadjah Mada1
Aurelia Graceilla Putri NindyatamaPelita Harapan University1
Moses Amadeus SoetantoAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/MKG/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Cytotoxicity test of 40, 50 and 60% citric acid as dentin conditioner by using MTT assay on culture cell line</dc:title>
	<dc:creator>Khoswanto, Christian</dc:creator>
	<dc:creator>Arijani, Ester</dc:creator>
	<dc:creator>Soesilawati, Pratiwi</dc:creator>
	<dc:subject xml:lang="en-US">cytotoxicity</dc:subject>
	<dc:subject xml:lang="en-US">citric acid</dc:subject>
	<dc:subject xml:lang="en-US">MTT assay</dc:subject>
	<dc:description xml:lang="en-US">Background: Open dentin is always covered by smear layer, therefore before restoration is performed, cavity or tooth which has been prepared should be clean from dirt. The researchers suggested that clean dentin surface would reach effective adhesion between resin and tooth structure, therefore dentin conditioner like citric acid was used to reach the condition. Even though citric acid is not strong acid but it can be very erosive to oral mucous. Several requirements should be fulfilled for dental product such as non toxic, non irritant, biocompatible and should not have negative effect against local, systemic or biological environment. Cytotoxicity test was apart of biomaterial evaluation and needed for standard screening. Purpose: This study was to know the cytotoxicity of 40, 50, 60% citric acid as dentin conditioner using MTT assay. Method: This study is an experimental research using the Post-Test Only Control Group Design. Six samples of each 40, 50 and 60% citric acid for citotoxicity test using MTT assay. The density of optic formazan indicated the number of living cells. All data were statistically analyzed by one way ANOVA. Result: The percentage of living cells in 40, 50 and 60% citric acid were 95.14%, 93.42% and 93.14%. Conclusion: Citric acid is non toxic and safe to be used as dentine conditioner.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en</dc:publisher>
	<dc:date>2008-09-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/MKG/article/view/992</dc:identifier>
	<dc:identifier>10.20473/j.djmkg.v41.i3.p103-106</dc:identifier>
	<dc:source xml:lang="en-US">Dental Journal (Majalah Kedokteran Gigi); Vol. 41 No. 3 (2008): September 2008; 103-106</dc:source>
	<dc:source>2442-9740</dc:source>
	<dc:source>1978-3728</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/MKG/article/view/992/775</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 Dental Journal (Majalah Kedokteran Gigi)</dc:rights>
</oai_dc:dc>