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The differences of effectiveness of β-1,3-glukanase Vigna unguiculata and papain Carica papaya enzymes in hydrolysis of denture plaque

Dental Journal (Majalah Kedokteran Gigi) · Vol. 49 No. 2 (2016) · hlm. 81–86

DOI
10.20473/j.djmkg.v49.i2.p81-86
ISSN
2442-9740, 1978-3728
Terbit
2016-06-30
Penerbit
Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en
Bahasa
eng

Abstrak

Background: Accumulation of denture plaque can lead to pathological changes in oral mucosa, such as denture stomatitis, halitosis, and caries. Plaque matrix is mostly formed by protein (30%) and polysaccharide complexes. Thus, an alternative enzyme solution as denture cleanser is required for hydrolysis of denture plaque. Papain is a proteolytic enzyme hydrolyzing proteins, while β-1,3-glucanase is a hydrolase enzyme hydrolyzing polysaccharides. Purpose: This study aimed to analyze the differences of effectiveness of íŸ-1,3- glucanase Vigna unguiculata enzyme and papain Carica papaya enzyme in hydrolysis of denture plaque. Method: This research was a laboratory experimental research with post test only control group design. After using denture for 24 hours, the denture was soaked in a solution of 100 ml PBS, papain enzyme, and β 1-3 glucanase enzyme at a concentration of 0.5 mg/ml, 1 mg/ml, and 2 mg/ml for 10 minutes. The solution from plaque hydrolysis was soaked in PBS and vortex enzyme for 2 minutes, then soaked in ice water for 15 minutes, and centrifuged at 3000 rpm 5-10º for 10 minutes. The supernatant was separated and analyzed. Turbidity readings then were performed in spectrofotometer with a wavelength of 480 nm. Result: 2 mg/ml of íŸ-1,3 glucanase enzyme generated the highest values of hydrolysis with a mean percentage of 68.77% compared to papain enzyme (44.86 %). The lowest values of hydrolysis weregenerated by PBS with a mean percentage of 3.24%. Conclusion: íŸ-1,3-glucanase enzyme is more effective in hydrolysis of denture plaque than papain enzyme.

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Indah Listiana KriswandiniAfiliasi tidak diketahui2
Retno Pudji RahayuAfiliasi tidak diketahui2
Ira ArundinaAfiliasi tidak diketahui2
Sitti Wahidah MasnaniHasanuddin University1
Mujadilah NurAfiliasi tidak diketahui1
Muhammad LuthfiAfiliasi tidak diketahui1
Sumarno SumarnoAfiliasi tidak diketahui1
Martien Herna SusantiAfiliasi tidak diketahui1
Fitriah Hasan ZabaAfiliasi tidak diketahui1
Elly MunadzirohAfiliasi tidak diketahui1
Agni Febrina PargaputriAfiliasi tidak diketahui1
Regina Purnama Dewi IskandarAfiliasi tidak diketahui1
Tuti KusumaningsihAfiliasi tidak diketahui1
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Deby Kania Tri PutriAfiliasi tidak diketahui1
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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/MKG/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">The differences of effectiveness of β-1,3-glukanase Vigna unguiculata and papain Carica papaya enzymes in hydrolysis of denture plaque</dc:title>
	<dc:creator>Indrawati, Retno</dc:creator>
	<dc:creator>Luthfi, Muhammad</dc:creator>
	<dc:creator>Andari, Erina Fatmala Yuli</dc:creator>
	<dc:subject xml:lang="en-US">Papain</dc:subject>
	<dc:subject xml:lang="en-US">íŸ-1</dc:subject>
	<dc:subject xml:lang="en-US">3-glucanase</dc:subject>
	<dc:subject xml:lang="en-US">denture plaque</dc:subject>
	<dc:description xml:lang="en-US">Background: Accumulation of denture plaque can lead to pathological changes in oral mucosa, such as denture stomatitis, halitosis, and caries. Plaque matrix is mostly formed by protein (30%) and polysaccharide complexes. Thus, an alternative enzyme solution as denture cleanser is required for hydrolysis of denture plaque. Papain is a proteolytic enzyme hydrolyzing proteins, while β-1,3-glucanase is a hydrolase enzyme hydrolyzing polysaccharides. Purpose: This study aimed to analyze the differences of effectiveness of íŸ-1,3- glucanase Vigna unguiculata enzyme and papain Carica papaya enzyme in hydrolysis of denture plaque. Method: This research was a laboratory experimental research with post test only control group design. After using denture for 24 hours, the denture was soaked in a solution of 100 ml PBS, papain enzyme, and β 1-3 glucanase enzyme at a concentration of 0.5 mg/ml, 1 mg/ml, and 2 mg/ml for 10 minutes. The solution from plaque hydrolysis was soaked in PBS and vortex enzyme for 2 minutes, then soaked in ice water for 15 minutes, and centrifuged at 3000 rpm 5-10º for 10 minutes. The supernatant was separated and analyzed. Turbidity readings then were performed in spectrofotometer with a wavelength of 480 nm. Result: 2 mg/ml of íŸ-1,3 glucanase enzyme generated the highest values of hydrolysis with a mean percentage of 68.77% compared to papain enzyme (44.86 %). The lowest values of hydrolysis weregenerated by PBS with a mean percentage of 3.24%. Conclusion: íŸ-1,3-glucanase enzyme is more effective in hydrolysis of denture plaque than papain enzyme.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en</dc:publisher>
	<dc:date>2016-06-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/MKG/article/view/1877</dc:identifier>
	<dc:identifier>10.20473/j.djmkg.v49.i2.p81-86</dc:identifier>
	<dc:source xml:lang="en-US">Dental Journal (Majalah Kedokteran Gigi); Vol. 49 No. 2 (2016): June 2016; 81-86</dc:source>
	<dc:source>2442-9740</dc:source>
	<dc:source>1978-3728</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/MKG/article/view/1877/2452</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2017 Dental Journal (Majalah Kedokteran Gigi)</dc:rights>
</oai_dc:dc>